Plant Resistance Mechanisms to Air Pollutants: Rhythms in Ascorbic Acid Production During Growth Under Ozone Stress

Lee, Edward H.

doi:10.3109/07420529109059161

Cited by 23 publications

(11 citation statements)

References 28 publications

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“…their relative concentrations), rather than by the absolute concentration of AA. It is well established that levels of both AA and DHA may change in response to environmental stresses (Castillo and Greppin 1988;Lee 1991;Badiani et al 1993;Walker and McKersie 1993;Brown et al 1995;Gogorcena et al 1995;Ranieri et al 1996;Foyer et al 1997;Iturbe-Ormaetxe et al 1998;Nector and Foyer 1998;Burkey 1999;Gara et al 2000;Shalata et al 2001). It has been reported frequently that water stress can lead to a significant decrease in AA content (Mukherjee and Choudhuri 1983;Sgherri and NavariIzzo 1995;Iturbe-Ormaetxe et al 1998;Bartoli et al 1999).…”

Section: Discussionmentioning

confidence: 95%

Regulation of Water Deficit-Induced Abscisic Acid Accumulation by Apoplastic Ascorbic Acid in Maize Seedlings

Gao

et al. 2005

J Integrative Plant Biology

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Water deficit-induced abscisic acid (ABA) accumulation is one of the most important stress signaling pathways in plant cells. Redox regulation of cellular signaling has currently attracted particular attention, but much less is known about its roles and mechanisms in plant signaling. Herein, we report that water deficit-induced ABA accumulation could be regulated by ascorbic acid (AA)-controlled redox status in leave apoplast. The AA content in non-stressed leaves was approximately 3 µmol/g FW, corresponding to a mean concentration of 3 mmol/L in a whole cell. Because AA is mainly localized in the cytosol and chloroplasts, the volume of which is much smaller than that of the whole cell, AA content in cytosolic and chloroplast compartments should be much higher than 3 mmol/L. Water deficit-induced ABA accumulation in both leaf and root tissues of maize seedlings was significantly inhibited by AA and reduced glutathione (GSH) at concentrations of 500 µmol/L and was completely blocked by 50 mmol/L AA and GSH. These results suggest that the AA-induced inhibition of ABA accumulation should not occur at sites where AA exists in high concentrations. Although water deficit led to a small increase in the dehydroascorbic acid (DHA) content, no significant changes in AA content were observed in either leaf or root tissues. When compared with the whole leaf cell, the AA content in the apoplastic compartment was much lower (i.e. approximately 70 nmol/g FW, corresponding to 0.7 mmol/L). Water deficit induced a significant decrease (approximately 2.5-fold) in the AA content and an increase (approximately 3.4-fold) in the DHA content in the apoplastic compartment, thus leading to a considerably decreased redox status there, which may have contributed to the relief of AA-induced inhibition of ABA accumulation, alternatively, promoting water deficit-induced ABA accumulation. Reactive oxygen species (ROS) could not mimic water deficit in inducing ABA accumulation, suggesting that the inhibition of ABA accumulation by AA or GSH was not related to their ROS-scavenging ability. The results of the present study suggest that the redox status in the apoplastic compartment, as determined by AA and DHA, may play a vital role in the regulation of the signaling process for water deficit-induced ABA accumulation.

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Section: Discussionmentioning

confidence: 95%

Regulation of Water Deficit-Induced Abscisic Acid Accumulation by Apoplastic Ascorbic Acid in Maize Seedlings

Gao

et al. 2005

J Integrative Plant Biology

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“…The justi®cation for this simplistic approach arises from the fact that: (i) leaf ASC concentration is inversely related to O 3 sensitivity (Freebairn 1960;Freebairn and Taylor 1960;Menser 1964;Lee et al 1984;Lee 1991;MaÈ chler et al 1995;Conklin et al 1996) and (ii) exposure of model solutions and biological¯uids to O 3 have revealed that ASC tops the hierarchical series of reaction targets in the aqueous matrix of the cell wall (Cross et al 1992;Kanofsky and Sima 1995a;Van der Vliet et al 1995;Mudway and Kelly 1998). However, there remain several uncertainties.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, considerable attention has recently focused on antioxidative systems in the cell wall, and in particular, the role of apoplastic ascorbate (ASC) in the scavenging of O 3 (Kelly et al 1995;Dietz 1997;Cross et al 1998;Lyons et al 1999a). L-ascorbate (vitamin C) is an abundant and powerful antioxidant, accumulating to millimolar concentrations in plant tissue (Loewus 1980(Loewus , 1988Smirno 1996;Noctor and Foyer 1998) and an inverse relationship is known to exist between leaf ASC concentrations and the sensitivity of plants to O 3 (Freebairn 1960;Freebairn and Taylor 1960;Menser 1964;Lee et al 1984;Lee 1991;MaÈ chler et al 1995;Conklin et al 1996). A small fraction (%1%) of the ASC pool is located in the cell wall, but there is evidence to suggest that the size, and rate of turnover of the ASC in this compartment, may be sucient to aord a signi®cant degree of protection against O 3 (Castillo and Greppin 1988;Luwe and Heber 1995;Polle et al 1995;Moldau et al 1997Moldau et al , 1998TurcsaÂ nyi et al 1999b;Lyons et al 1999a).…”

Section: Introductionmentioning

confidence: 99%

Simulating ozone detoxification in the leaf apoplast through the direct reaction with ascorbate

Plöchl

Lyons

Ollerenshaw

et al. 2000

Planta

126

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This paper presents a mathematical model which enables the semi-quantification of ozone (O3) detoxification, based upon the direct reaction of the pollutant with ascorbate (ASC) located in the aqueous matrix associated with the cell wall (i.e. the apoplast). The model describes the uptake of ozone into the leaf and its direct reaction with ASC, taking into consideration the regeneration of dehydroascorbic acid in the cytosol, the rate of replenishment of cell wall ASC and the distribution of ASC between sub-cellular compartments based upon the permeability of biomembranes to the neutral species, ascorbic acid and the pH of various sub-cellular compartments. The importance of various physico-chemical characteristics (e.g. stomatal conductance, mesophyll cell wall thickness and tortuosity, chloroplast volume, apoplast pH, ASC:O3 reaction stoichiometry) in mediating the flux of ozone to the plasmalemma is analysed. Model simulations, supported by experimental observations, suggest that the ASC concentration in the leaf apoplast is high enough to scavenge a significant proportion of the O3 taken up into the leaf interior, under environmentally relevant conditions. However, there is considerable variation between taxa in the potential degree of protection afforded by apoplastic ASC, emphasizing the need for an improved understanding of the reaction chemistry of O3 in the cell wall.

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“…Plants with high ascorbate concentrations in their tissues have proven to be more resistant to oxidative damage by ozone than plants with low ascorbate concentrations (Lee et al 1984;Lee 1991). Particular cultivars of common bean maintaining higher ascorbate concentrations in the leaf apoplast were less affected by ozone treatment (Burkey and Eason 2002).…”

Section: Introductionmentioning

confidence: 97%

Over-expression of l-gulono-γ-lactone oxidase (GLOase) gene leads to ascorbate accumulation with enhanced abiotic stress tolerance in tomato

Lim

Pulla

Park

et al. 2012

In Vitro Cell.Dev.Biol.-Plant

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Vitamin C (ascorbic acid, AsA) is an essential component for collagen biosynthesis and also for correct functioning of the cardiovascular system in humans. Plants and several animals can synthesize ascorbic acid, whereas humans lack the gene essential for ascorbic acid biosynthesis and must acquire the vitamin from their diet. In the present study, we developed a transgenic tomato plant (Lycopersicon esculentum Mill.) over-expressing the rat L-gulono-γ-lactone oxidase (GLOase) gene driven by CaMV35S constitutive promoter. The overexpression of GLOase resulted in a 1.5-fold increase in AsA levels in transgenic tomato fruits, which was positively correlated with increased GLOase activity. The Southern and reverse transcriptase polymerase chain reaction results revealed the stable integration of the transgene in the progeny of the engineered plants. The transgenic plants with increased vitamin C content in the fruit showed enhanced tolerance to abiotic stresses induced by methyl viologen, NaCl, and mannitol as compared with wild-type plants. The leaf disc senescence assay showed better tolerance in transgenic plants by retaining higher chlorophyll content compared with wild-type plants. The T 1 plants survived under salt stress (200 mM NaCl) whereas wild-type plants were unable to survive such high salt concentrations. This study suggests that the increased accumulation of AsA could upregulate the antioxidant system which imparts improved tolerance against various abiotic stresses in transgenic tomato plants as compared with wild-type plants.

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Plant Resistance Mechanisms to Air Pollutants: Rhythms in Ascorbic Acid Production During Growth Under Ozone Stress

Cited by 23 publications

References 28 publications

Regulation of Water Deficit-Induced Abscisic Acid Accumulation by Apoplastic Ascorbic Acid in Maize Seedlings

Regulation of Water Deficit-Induced Abscisic Acid Accumulation by Apoplastic Ascorbic Acid in Maize Seedlings

Simulating ozone detoxification in the leaf apoplast through the direct reaction with ascorbate

Over-expression of l-gulono-γ-lactone oxidase (GLOase) gene leads to ascorbate accumulation with enhanced abiotic stress tolerance in tomato

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