Plant Responses of Maize to Two formae speciales of Sporisorium reilianum Support Recent Fungal Host Jump

Abstract

Host jumps are a major factor for the emergence of new fungal pathogens. In the evolution of smut fungi, a putative host jump occurred in Sporisorium reilianum that today exists in two host-adapted formae speciales, the sorghum-pathogenic S. reilianum f. sp. reilianum and maize-pathogenic S. reilianum f. sp. zeae. To understand the molecular host-specific adaptation to maize, we compared the transcriptomes of maize leaves colonized by both formae speciales. We found that both varieties induce many common defense response-associated genes, indicating that both are recognized by the plant as pathogens. S. reilianum f. sp. reilianum additionally induced genes involved in systemic acquired resistance. In contrast, only S. reilianum f. sp. zeae induced expression of chorismate mutases that function in reducing the level of precursors for generation of the defense compound salicylic acid (SA), as well as oxylipin biosynthesis enzymes necessary for generation of the SA antagonist jasmonic acid (JA). In accordance, we found reduced SA levels as well as elevated JA and JA-Ile levels in maize leaves inoculated with the maize-adapted variety. These findings support a model of the emergence of the maize-pathogenic variety from a sorghum-specific ancestor following a recent host jump.

Genetic and Pathogenic Variability among Isolates of Sporisorium reilianum Causing Sorghum Head Smut

“…reilianum, which infects sorghum, and S. reilianum f. sp. zeae, which causes head smut on maize [6][7][8][9]. In this study, pathogenicity, which refers to the ability to cause disease in the host, and virulence, which indicates either the degree or worsening of the disease [10], will be used interchangeably.…”