Plant Small Heat Shock Proteins and Its Interactions with Biotic Stress

Lopes-Caitar, Valéria Stefania; Silva, Suellen Mika Hishinuma; Marcelino-Guimarães, Francismar Corrêa

doi:10.1007/978-3-319-46340-7_2

Cited by 10 publications

(4 citation statements)

References 79 publications

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“…Several heat-shock proteins (HSPs) which normally accumulate under diverse abiotic stress conditions [ 46 ] were repressed following P. cinnamomi infection in the stem sections proximal and distal to the necrotic zone. Apart from their role in abiotic stress, HSPs are important players in defense signaling during pathogen attacks by modulating the stability and accumulation of resistance proteins [ 47 ]. Silencing of small HSPs has been linked to a decrease in the expression of defense-related genes, such as PR1 and PR4 in tobacco, and the perturbation of SA signaling [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut (Castanea sativa) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It

Saiz-Fernández

Milenković

Berka

et al. 2020

IJMS

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Phytophthora cinnamomi is one of the most invasive tree pathogens that devastates wild and cultivated forests. Due to its wide host range, knowledge of the infection process at the molecular level is lacking for most of its tree hosts. To expand the repertoire of studied Phytophthora–woody plant interactions and identify molecular mechanisms that can facilitate discovery of novel ways to control its spread and damaging effects, we focused on the interaction between P. cinnamomi and sweet chestnut (Castanea sativa), an economically important tree for the wood processing industry. By using a combination of proteomics, metabolomics, and targeted hormonal analysis, we mapped the effects of P. cinnamomi attack on stem tissues immediately bordering the infection site and away from it. P. cinnamomi led to a massive reprogramming of the chestnut proteome and accumulation of the stress-related hormones salicylic acid (SA) and jasmonic acid (JA), indicating that stem inoculation can be used as an easily accessible model system to identify novel molecular players in P. cinnamomi pathogenicity.

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Section: Discussionmentioning

confidence: 99%

Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut (Castanea sativa) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It

Saiz-Fernández

Milenković

Berka

et al. 2020

IJMS

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“…As chaperones, sHSP proteins have been described as induced by different biotic and abiotic stress, including nematode infection [ 12 , 14 , 15 ]. In this study, we examined specifically the role of the Gm Hsp 22.4 gene in the M. javanica resistance response in details.…”

Section: Discussionmentioning

confidence: 99%

“…In plants, biotic and abiotic stresses can trigger diverse defense mechanisms, such as the activation of a group of highly conserved proteins known as heat shock proteins (HSPs) [ 10 ]. The main function of the HSPs is to act as molecular chaperones, performing maintenance of the spatial structure of other proteins that are negatively affected by changes in factors such as temperature [ 11 , 12 ]. These proteins were initially observed in the salivary glands of Drosophila spp.…”

Section: Introductionmentioning

confidence: 99%

The soybean gene GmHsp22.4 is involved in the resistance response to Meloidogyne javanica in Arabidopsis thaliana

et al. 2020

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Background Small heat shock proteins (sHSPs) belong to the class of molecular chaperones that respond to biotic and abiotic stresses in plants. A previous study has showed strong induction of the gene GmHsp22.4 in response to the nematode Meloidogyne javanica in a resistant soybean genotype, while repression in a susceptible one. This study aimed to investigate the functional involvement of this small chaperone in response to M. javanica in Arabidopsis thaliana. First, it was evaluated the activation of the promoter region after the nematode inoculation, and the occurrence of polymorphisms between resistant and susceptible re-sequenced soybean accessions. Then functional analysis using A. thaliana lines overexpressing the soybean GmHsp22.4 gene, and knocked-out mutants were challenged with M. javanica infestation. Results High expression levels of the GFP gene marker in transformed A. thaliana plants revealed that the promoter region of GmHsp22.4 was strongly activated after nematode inoculation. Moreover, the multiplication of the nematode was significantly reduced in plants overexpressing GmHsp22.4 gene in A. thaliana compared to the wild type. Additionally, the multiplication of M. javanica in the A. thaliana mutants was significantly increased mainly in the event athsp22.0–2. This increase was not that evident in the event athsp22.0–1, the one that preserved a portion of the promoter region, including the HSEs in the region around − 83 bp. However, structural analysis at sequence level among soybean resistant and susceptible genotypes did not detect any polymorphisms in the whole gene model. Conclusions The soybean chaperone GmHsp22.4 is involved in the defense response to root-knot nematode M. javanica in A. thaliana. Specifically, the promoter region covering until − 191 from the transcriptional start site (TSS) is necessary to promoter activation after nematode infection in Arabidopsis. No polymorphisms that could explain these differences in the defense response were detected in the GmHsp22.4 gene between resistant and susceptible soybean genotypes. Therefore, further investigation is needed to elucidate the triggering factor of the plant’s defense mechanism, both at the sequence level of the soybean genotypes presenting contrasting reaction to root-knot nematode and by detecting cis-elements that are essential for the activation of the GmHsp22.4 gene promoter.

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“…In plants, biotic and abiotic stresses can trigger diverse defense mechanisms, such as the activation of a group of highly conserved proteins known as heat shock proteins (HSPs) [7]. The main function of the HSPs is to act as molecular chaperones, performing maintenance of the spatial structure of other proteins that are negatively affected by changes in factors such as temperature [8,9]. These proteins were initially observed in the salivary glands of Drosophila spp.…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of a soybean small heat shock protein involved in the resistance response to Meloidogyne javanica

Hishinuma-Silva

Lopes-Caitar

Nomura

et al. 2019

Preprint

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Background: Small heat shock proteins (sHSPs) belong to the class of molecular chaperones that respond to biotic and abiotic stresses in plants. Previous studies have identified strong induction of the GmHsp22.4 gene in response to Meloidogyne javanica nematode in resistant soybean genotypes compared to susceptible one. This study aimed to investigate the functional involvement of this small chaperone in response to M. javanica. First, it was evaluated the activation of the promoter region by nematode’s infection and the occurrence of polymorphisms between resistant and susceptible resequenced soybean accessions in the gene coding and promoter regions. Then functional analysis using Arabidopsis thaliana lines overexpressing the soybean GmHsp22.4 gene, and knocked out mutants were challenged to M. javanica’s infection.Results: High expression levels of the GFP gene marker in transformed A. thaliana plants revealed that the promoter region of GmHsp22.4 was strongly activated after nematode infection. However, structural analysis of the soybean resistant and susceptible genotypes did not detect any polymorphisms in the whole gene model, including the 2.0 kb promoter region. Moreover, the reproduction of the nematode was significantly reduced in plants overexpressing GmHsp22.4 gene in A. thaliana compared to the wild type. Additionally, the reproduction of M. javanica in the A. thaliana mutants was significantly increased and was related with the structural organization of heat shock cis element (HSE) in the promoter.Conclusions: The soybean chaperone GmHsp22.4 is involved in the defense response to root-knot nematode M. javanica’s infection in A. thaliana. The HSE in the promoter region close to the transcriptional start site (TSS) are important to GmHsp22.4 promoter activation after nematode infection. It was not possible to detect any polymorphisms occurrence in GmHsp22.4 gene between the M. javanica-resistant and susceptible soybean genotypes, so the resistance mechanism might not be related with the transcriptional regulation in the initiation process or based on sequence level on the TSS.

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Plant Small Heat Shock Proteins and Its Interactions with Biotic Stress

Cited by 10 publications

References 79 publications

Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut (Castanea sativa) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It

Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut (Castanea sativa) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It

The soybean gene GmHsp22.4 is involved in the resistance response to Meloidogyne javanica in Arabidopsis thaliana

Functional characterization of a soybean small heat shock protein involved in the resistance response to Meloidogyne javanica

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