Plant Species Successfully Micropropagated Photoautotrophically

Kubota, Chieri; Afreen, F.; Zobayed, S. M. A.

doi:10.1007/1-4020-3126-2_15

Cited by 5 publications

(3 citation statements)

References 97 publications

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“…The basal stem of microshoots treated with 50 mg/L IBA and 20 mg/L NAA was used for the transcriptome analysis. This is the first study of genes associated with AR formation in sugarcane microshoots grown under photoautotrophic conditions [21]. Since there is no fully annotated high-quality reference genome published for commercial sugarcane varieties, the de-novo assembly of Illumina short reads was conducted in this study.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Profiling Provides Molecular Insights into Auxin-Induced Adventitious Root Formation in Sugarcane (Saccharum spp. Interspecific Hybrids) Microshoots

Lakshmanan

et al. 2020

Plants

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Adventitious root (AR) formation was enhanced following the treatment of sugarcane microshoots with indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) combined, suggesting that auxin is a positive regulator of sugarcane microshoot AR formation. The transcriptome profile identified 1737 and 1268 differentially expressed genes (DEGs) in the basal tissues (5 mm) of sugarcane microshoots treated with IBA+NAA compared to nontreated control on the 3rd and 7th days post-auxin or water treatment (days post-treatment—dpt), respectively. To understand the molecular changes, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. This analysis showed that DEGs associated with the pathways were associated with plant hormone signaling, flavonoid and phenylpropanoid biosyntheses, cell cycle, and cell wall modification, and transcription factors could be involved in sugarcane microshoot AR formation. Furthermore, qRT–PCR analysis was used to validate the expression patterns of nine genes associated with root formation and growth, and the results were consistent with the RNA-seq results. Finally, a hypothetical hormonal regulatory working model of sugarcane microshoot AR formation is proposed. Our results provide valuable insights into the molecular processes associated with auxin-induced AR formation in sugarcane.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Profiling Provides Molecular Insights into Auxin-Induced Adventitious Root Formation in Sugarcane (Saccharum spp. Interspecific Hybrids) Microshoots

Lakshmanan

et al. 2020

Plants

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“…Pada sistem autotropik ini akan cenderung membuat eksplan atau tanaman tidak bergantung pada ketersediaan sukrosa dalam media kultur, melainkan bergantung pada CO 2 sebagai sumber energinya dalam proses fotosintesis. Apabila konsentrasi CO2 rendah pada botol kultur maka dapat mendorong eksplan menjadi heterotrof dan jika terpapar oleh radiasi yang terlalu tinggi dapat menigkatkan kerentanan tanaman terhadap stress oksidatif yang dapat memicu terhambatnya pertumbuhan dan perkembangan eksplan (Kubota 2002).…”

Section: Waktu Munculnya Tunas Aksilarunclassified

Perbanyakan Tanaman Chrysanthemum pada Kondisi Fotoautotropik Secara in Vitro

Apriliani,

Widyajayantie,

Hidayah

et al. 2023

Agiotech

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Perbanyakan bibit melalui kultur jaringan yang dilakukan dapat memberikan solusi untuk meningkatkan ketersediaan bibit krisan (Chrysanthemum sp.) yang berkualitas, terbebas dari virus maupun hama penyakit, dapat menghasilkan bibit dalam jumlah banyak dengan waktu yang singkat, dan bersifat seragam. Pengendalian lingkungan in vitro bertujuan untuk menghasilkan tanaman yang lebih mudah untuk beradaptasi pada lingkungan ex vitro salah satunya melalui sistem kultur fotoautotropik. Pada penelitian ini dilakukan studi mengenai keefektifan modifikasi media dan kondisi kultur menggunakan sistem autotropik. Penelitian dilakukan secara deskriptif dengan pengujian tiga jenis media MS + 30 g/L gula + 2 mg/L CaP (M1); MS + 30 g/L gula + 0.1 mg/L NAA + 1 mg/L BAP (M2); dan MS + 15 g/L gula + 0.1 mg/L NAA + 1 mg/L BAP (M3). Serta pemberian 1 lubang ventilasi (V1); 2 lubang ventilasi (V2). Hasil penelitian menunjukkan bahwa peningkatan konsentrasi gula dapat meningkatkan pertumbuhan tunas, namun bila dalam jumlah banyak juga dapat menghambat pertumbuhan tunas aksilar. Penggunaan lubang ventilasi pada sistem autotropik dapat menyebabkan terjadinya kontaminasi yang lebih banyak, hal ini dapat dikurangi dengan mengkontrol sterilitas eksplan yang digunakan serta lingkungan yang sesuai seperti kelembaban, suhu, dan intensitas cahaya yang sesuai

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“…24,27 Enhanced plantlet growth and multiplication under controlled carbon dioxide enriched environment have been achieved in a number of herbaceous, 28,29 and woody plants. [30][31][32][33][34][35][36] The present study was undertaken hypothesizing that the liquid medium and CO 2 enrichment could synergistically affect the in vitro growth of C. paniculatus, both in terms of rate of multiplication as well as their photosynthetic efficiency. It was also assumed that the plantlets obtained would be of better quality having strong potential of their survival during ex vitro transplantation.…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Habibi¹,

Purohit²

2019

Int J Phytocos Nat Ingred

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Introduction: Celastrus paniculatus (family: Celastraceae) is a woody climbing shrub valued for its immense medicinal properties contained in its various plant parts. Over-exploitation and poor natural regeneration either by seed or other method/s have resulted into depleting population of C. paniculatus in natural habitats in India. Novel approaches such as liquid culture media and photoautotrophic multiplication of shoots on sugar-free media has proved useful to obtain photosynthetically active micropropagated plantlets. Methods: The shoot cultures of C. paniculatus were multiplied on sucrose containing and sucrose-free semi-solid and liquid media. The cultures were further incubated under various concentrations of carbon dioxide (0.0 to 40.0 gm-3). The assessment of chlorophyll fluorescence parameters (F o , F m , F' m , F t , F v /F m and ΦPS2) under similar conditions was undertaken. Results: It was observed that CO 2 enrichment favored shoot multiplication and elongation and biomass production in sucrose supplemented medium. CO 2 (10.0 gm-3) along with sucrose (3.0%), recorded maximum growth on semi-solid and liquid media. The valuation of F o , F m , F' m , F t , F v /F m and ΦPS2 revealed that an increase in the concentration of CO 2 resulted in a decline in all the parameters especially the F v / F m and ΦPS2. On the contrary, withdrawal of sucrose from the medium under CO 2 enriched conditions resulted in a moderate growth rate and biomass production. However, F v /F m and ΦPS2 were considerably improved in shoot cultures incubated under elevated concentrations of CO 2 (10.0 gm-3) without sucrose in the medium indicating their photoautotrophic growth. Conclusion: Liquid medium proved to be superior for overall growth and biomass production over its semi-solid counterparts. The observations of photochemical efficiency in shoot cultures grown on liquid medium were at par with their semi-solid counterparts indicating no adverse effects such as hyperhydricity.

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Plant Species Successfully Micropropagated Photoautotrophically

Cited by 5 publications

References 97 publications

Transcriptome Profiling Provides Molecular Insights into Auxin-Induced Adventitious Root Formation in Sugarcane (Saccharum spp. Interspecific Hybrids) Microshoots

Transcriptome Profiling Provides Molecular Insights into Auxin-Induced Adventitious Root Formation in Sugarcane (Saccharum spp. Interspecific Hybrids) Microshoots

Perbanyakan Tanaman Chrysanthemum pada Kondisi Fotoautotropik Secara in Vitro

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

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