Plant-Specific Histone Deacetylases HDT1/2 Regulate <i>GIBBERELLIN 2-OXIDASE2</i> Expression to Control Arabidopsis Root Meristem Cell Number

Li, Huchen; Torres-Garcia, Sito; Latrasse, David; Benhamed, Moussa; Schilderink, Stefan; Zhou, Wenkun; Kulikova, Olga; Hirt, Heribert; Bisseling, Ton

doi:10.1105/tpc.17.00366

Cited by 75 publications

(76 citation statements)

References 74 publications

(109 reference statements)

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“…Comparison of sequences upstream of the predicted transcription start sites between mutant and the wild type did not reveal any characteristic small deletions that are typical following treatment with fast neutron radiation ( Supplemental Table S1; Koornneef et al, 1982). Changes in chromatin structure were recently linked to the regulation of GA 2-oxidase gene in Arabidopsis root meristems (Li et al, 2017b). Future work will investigate the molecular basis of increased expression of GA2oxA9 in Icaro and Castelporziano.…”

Section: Discussionmentioning

confidence: 97%

Rht18 Semidwarfism in Wheat Is Due to Increased GA 2-oxidaseA9 Expression and Reduced GA Content

et al. 2018

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Semidwarfing genes have improved crop yield by reducing height, improving lodging resistance, and allowing plants to allocate more assimilates to grain growth. In wheat (), the semidwarfing gene was identified and deployed in durum wheat before it was transferred into bread wheat, where it was shown to have agronomic potential., a dominant and gibberellin (GA) responsive mutant, is genetically and functionally distinct from the widely used GA-insensitive semidwarfing genes and In this study, the gene was identified by mutagenizing the semidwarf durum cultivar Icaro () and generating mutants with a range of tall phenotypes. Isolating and sequencing chromosome 6A of these "overgrowth" mutants showed that they contained independent mutations in the coding region of is predicted to encode a GA 2-oxidase that metabolizes GA biosynthetic intermediates into inactive products, effectively reducing the amount of bioactive GA (GA). Functional analysis of the GA2oxA9 protein demonstrated that GA2oxA9 converts the intermediate GA to the inactive metabolite GA Furthermore, showed higher expression of and lower GA content compared with its tall parent. These data indicate that the increased expression of in results in a reduction of both bioactive GA content and plant height. This study describes a height-reducing mechanism that can generate new genetic diversity for semidwarfism in wheat by combining increased expression with mutations of specific amino acid residues in .

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Section: Discussionmentioning

confidence: 97%

Rht18 Semidwarfism in Wheat Is Due to Increased GA 2-oxidaseA9 Expression and Reduced GA Content

et al. 2018

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“…ChIP experiments were performed as described (Li et al, 2017). In brief, approximately 1 gram of wild-type Col-0 (control) and 35s::MYC2-GFP seedlings at 5 DAG were used for each anti-GFP ChIP assay.…”

Section: Chip-qpcr Assaymentioning

confidence: 99%

A Jasmonate Signaling Network Activates Root Stem Cells and Promotes Regeneration

Zhou

Lozano‐Torres

Blilou

et al. 2019

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Plants are sessile and have to cope with environmentally induced damage through modification of growth and defense pathways. It is an open question how tissue regeneration is triggered in such responses and whether this involves stem cell activation. The stress hormone jasmonate (JA) plays well-established roles in wounding and defense responses. JA also affects growth which is hitherto interpreted as trade-off between growth and defense. Here, we describe a molecular network triggered by wound-induced JA that promotes stem cell activation and regeneration. JA regulates organizer cell activity in the root stem cell niche through the RBR-SCR network and stress response protein ERF115. Moreover, JA-induced ERF109 transcription stimulates CYCD6;1 expression, functions upstream of ERF115 and promotes regeneration. Soil penetration and response to nematode herbivory induce and require this JA mediated regeneration response. Therefore, the JA tissue damage response pathway induces stem cell activation and regeneration, and activates growth after environmental stress. Results JA acts through RBR-SCR to regulate quiescenceWe confirmed that JA promotes division of QC cells (Chen et al., 2011) ( Figures 1A and 1B, S1B and S1C), and that extra QC divisions occur in plants with altered SCR-RBR network:pWOX5::amiGORBR, which specifically downregulates RBR in the QC, and pSCR::SCRaca-YFP, which specifically disrupts RBR-SCR protein interaction (Cruz-Ramirez et al., 2013) ( Figures 1D and 1G). We observed QC divisions in wild type Col-0 after 50 µM or 100 µM MeJA (methyl ester of JA) treatment for 48 hours ( Figures 1A-1C and 1J), respectively. Conversely, no extra QC divisions were detected in pWOX5::amiGORBR and pSCR::SCRaca-YFP lines treated with 50 µM or 100 µM MeJA for the same period (Figures 1D-1I and 1K-1L), suggesting that JA may function through the SCR-RBR network in regulating quiescence of QC. To further confirm this, we introgressed the JA receptor loss of function mutant allele coi1-2 (Xu et al., 2002) into pWOX5::amiGORBR and pSCR::SCRaca-YFP lines. The QC division phenotypes and QC cell numbers of these introgression lines revealed no differences to pSCR::SCRaca-YFP and pWOX5::amiGORBR lines, both with and without MeJA treatment, respectively ( Figures S1D-S1M). These findings position SCR and RBR downstream of COI1 to control quiescence of QC. After MeJA treatment, QC cell numbers of scr-3 and shr-2 mutants did not increase significantly compared to controls ( Figures S1N-S1T), confirming that JA induces QC division through the SCR/SHR pathway. SCR and RBR expression levels were not altered significantly after MeJA treatment, judged by marker expression and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays (Figures S1U-S1AA).These data indicate that JA does not regulate SCR and RBR at the gene expression level. JA directly induces ERF115 transcriptionThe AP2/ERF family transcription factor ERF115 was reported to control root QC cell division (Heyman et al., 2013). When 35S::ERF11...

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“…Plants have developed sophisticated mechanisms to withstand salt stress (Cui et al, 2015). Gibberellins (GAs) are tetracyclic diterpene phytohormones that affect plant growth and development (Schwechheimer, 2012;Davière and Achard, 2013;Hedden and Sponsel, 2015;Kwon and Paek, 2016;Wang et al, 2017) by promoting cell elongation (De-Lucas et al, 2008;Rizza et al, 2017) or cell division (Li et al, 2017) to control organ growth in the developmental context. The modulation of GA levels in response to various stresses including cold, salinity, drought stress, and submergence has been investigated over the past decade (Colebrook et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

The Ubiquitin-Binding Protein OsDSK2a Mediates Seedling Growth and Salt Responses by Regulating Gibberellin Metabolism in Rice

et al. 2019

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UBL-UBA (ubiquitin-like-ubiquitin-associated) proteins are ubiquitin receptors and transporters in the ubiquitin-proteasome system that play key roles in plant growth and development. High salinity restricts plant growth by disrupting cellular metabolism, but whether UBL-UBA proteins are involved in this process is unclear. Here, we demonstrate that the UBL-UBA protein OsDSK2a (DOMINANT SUPPRESSOR of KAR2) mediates seedling growth and salt responses in rice (Oryza sativa). Through analysis of osdsk2a, a mutant with retarded seedling growth, as well as in vitro and in vivo assays, we demonstrate that OsDSK2a combines with polyubiquitin chains and interacts with the gibberellin (GA)-deactivating enzyme ELONGATED UPPERMOST INTERNODE (EUI), resulting in its degradation through the ubiquitin-proteasome system. Bioactive GA levels were reduced, and plant growth was retarded in the osdsk2a mutant. By contrast, eui mutants displayed increased seedling growth and bioactive GA levels. OsDSK2a levels decreased in plants under salt stress. Moreover, EUI accumulated under salt stress more rapidly in osdsk2a than in wild-type plants. Thus, OsDSK2a and EUI play opposite roles in regulating plant growth under salt stress by affecting GA metabolism. Under salt stress, OsDSK2a levels decrease, thereby increasing EUI accumulation, which promotes GA metabolism and reduces plant growth.

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Plant-Specific Histone Deacetylases HDT1/2 Regulate GIBBERELLIN 2-OXIDASE2 Expression to Control Arabidopsis Root Meristem Cell Number

Cited by 75 publications

References 74 publications

Rht18 Semidwarfism in Wheat Is Due to Increased GA 2-oxidaseA9 Expression and Reduced GA Content

Rht18 Semidwarfism in Wheat Is Due to Increased GA 2-oxidaseA9 Expression and Reduced GA Content

A Jasmonate Signaling Network Activates Root Stem Cells and Promotes Regeneration

The Ubiquitin-Binding Protein OsDSK2a Mediates Seedling Growth and Salt Responses by Regulating Gibberellin Metabolism in Rice

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