Plant Transformation via Pollen Tube-Mediated Gene Transfer

Ali, Asjad; Bang, Sun Woong; Chung, Sang-Min; Staub, Jack E.

doi:10.1007/s11105-014-0839-5

Cited by 26 publications

(23 citation statements)

References 36 publications

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“…Pollen tube mediated transformation is efficient and simple alternative of producing transgenic plants, evading the stipulations for tissue culture. The PTT method was first reported in cotton (Gossypium hirsutum L.) [130] and rice [131]. Two approaches were as a naked DNA or through Agrobacterium.…”

Section: Transformation and Regeneration Of Cotyledonary Tissues Of Cmentioning

confidence: 99%

The Tale of Cotton Plant: From Wild Type to Domestication, Leading to Its Improvement by Genetic Transformation

Aslam¹,

Khan²,

Ahmed³

et al. 2020

AJMB

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Cotton is considered as a major cash crop of the world. It earns huge foreign exchange by its valuable products; fiber, lint, cotton seed oil, hull and a lot more. Being an important fiber crop, it earns huge foreign exchange by contributing to textile and seed oil industry. This review summarizes cotton biology, its diversity and domestication, genome assembly, constraints in its production and methods to improve cotton plant to fulfill the need of textile and oil industry. But cotton is facing enormous biotic and abiotic stresses with insect pests being most prominent. Massive destruction caused by insects needs to be controlled for maintaining fruitful cotton crop production. Conventional breeding approaches are limited to improving single trait and integrate stable genes within plant genome in approximately 7-8 years. Improved biotechnological procedures have paved new pathways to target genes specifically and improve cotton germplasm in lesser time than conventional breeding.

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Section: Transformation and Regeneration Of Cotyledonary Tissues Of Cmentioning

confidence: 99%

The Tale of Cotton Plant: From Wild Type to Domestication, Leading to Its Improvement by Genetic Transformation

Aslam¹,

Khan²,

Ahmed³

et al. 2020

AJMB

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“…Pollen transformations by Agrobacterium and biolistic method bypasses tissue culture regeneration step but frequently results in pollen with poor viability [ 105 , 106 ]. Furthermore, pollen-tube conversions can lead to chimerism [ 107 ]. While pollen magnetoreception has made progress in addressing these issues [ 106 ], it is still limited to dicots [ 108 ].…”

Section: Approaches To Applying Crispr/cas9 System In Plantsmentioning

confidence: 99%

CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops

et al. 2022

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Demand of flowers is increasing with time worldwide. Floriculture has become one of the most important commercial trades in agriculture. Although traditional breeding methods like hybridization and mutation breeding have contributed significantly to the development of important flower varieties, flower production and quality of flowers can be significantly improved by employing modern breeding approaches. Novel traits of significance have interest to consumers and producers, such as fragrance, new floral color, change in floral architecture and morphology, vase life, aroma, and resistance to biotic and abiotic stresses, have been introduced by genetic manipulation. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has recently emerged as a powerful genome-editing tool for accurately changing DNA sequences at specific locations. It provides excellent means of genetically improving floricultural crops. CRISPR/Cas system has been utilized in gene editing in horticultural cops. There are few reports on the utilization of the CRISPR/Cas9 system in flowers. The current review summarizes the research work done by employing the CRISPR/Cas9 system in floricultural crops including improvement in flowering traits such as color modification, prolonging the shelf life of flowers, flower initiation, and development, changes in color of ornamental foliage by genome editing. CRISPR/Cas9 gene editing could be useful in developing novel cultivars with higher fragrance and enhanced essential oil and many other useful traits. The present review also highlights the basic mechanism and key components involved in the CRISPR/Cas9 system.

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“…Agrobacterium and biolistic transformation of pollen also bypasses regeneration but often results in pollen with lower viability ( Wang et al, 2008 ; Zhao et al, 2017 ). In addition, pollen-tube transformations may result in chimerism ( Ali A. et al, 2015 ). While pollen magnetofection has improved on these drawbacks ( Zhao et al, 2017 ), its application remains constrained to dicots ( Vejlupkova et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Advances in Delivery Mechanisms of CRISPR Gene-Editing Reagents in Plants

Laforest

Nadakuduti

2022

Front. Genome Ed.

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Gene-editing by CRISPR/Cas systems has revolutionized plant biology by serving as a functional genomics tool. It has tremendously advanced plant breeding and crop improvement by accelerating the development of improved cultivars, creating genetic variability, and aiding in domestication of wild and orphan crops. Gene-editing is a rapidly evolving field. Several advancements include development of different Cas effectors with increased target range, efficacy, and enhanced capacity for precise DNA modifications with base editing and prime editing. The existing toolbox of various CRISPR reagents facilitate gene knockouts, targeted gene insertions, precise base substitutions, and multiplexing. However, the major challenge in plant genome-editing remains the efficient delivery of these reagents into plant cells. Plants have larger and more complex genome structures compared to other living systems due to the common occurrence of polyploidy and other genome re-arrangements. Further, rigid cell walls surrounding plant cells deter the entry of any foreign biomolecules. Unfortunately, genetic transformation to deliver gene-editing reagents has been established only in a limited number of plant species. Recently, there has been significant progress in CRISPR reagents delivery in plants. This review focuses on exploring these delivery mechanisms categorized into Agrobacterium-mediated delivery and breakthroughs, particle bombardment-based delivery of biomolecules and recent improvements, and protoplasts, a versatile system for gene-editing and regeneration in plants. The ultimate goal in plant gene-editing is to establish highly efficient and genotype-independent reagent delivery mechanisms for editing multiple targets simultaneously and achieve DNA-free gene-edited plants at scale.

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Plant Transformation via Pollen Tube-Mediated Gene Transfer

Cited by 26 publications

References 36 publications

The Tale of Cotton Plant: From Wild Type to Domestication, Leading to Its Improvement by Genetic Transformation

The Tale of Cotton Plant: From Wild Type to Domestication, Leading to Its Improvement by Genetic Transformation

CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops

Advances in Delivery Mechanisms of CRISPR Gene-Editing Reagents in Plants

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