Plant virus gene expression strategies

Bustamante, Pedro I.; Hull, Roger

doi:10.2225/vol1-issue2-fulltext-3

Cited by 4 publications

(3 citation statements)

References 128 publications

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“…Across the samples, we detected reads corresponding to all the genome organization types for viruses, with (-) ssRNA and dsRNA viruses being the least abundant (< 1% of all viral reads for samples in which they were detected). We further focused on ssRNA viruses, since 75% of known plant viruses have this genome organization [32]. Per sample, between 0.6% and 95.6% of reads assigned to viruses were indeed classified as ssRNA viruses.…”

Section: 1mentioning

confidence: 99%

Virome analysis of irrigation water sources provides extensive insights into the diversity and distribution of plant viruses in agroecosystems

Ferreira

Bačnik

Rivarez

et al. 2023

Preprint

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Plant viruses pose a significant threat to agriculture. Several are stable outside their hosts, can enter water bodies and remain infective for prolonged periods of time. Even though the quality of irrigation water is of increasing importance in the context of plant health, the presence of plant viruses in irrigation waters is understudied. In this study, we conducted a large-scale high-throughput sequencing (HTS)-based virome analysis of irrigation and groundwater sources to obtain complete information about the abundance and diversity of plant viruses in such waters. We detected nucleic acids of plant viruses from 20 families, discovered several novel plant viruses from economically important taxa, like Tobamovirus and observed the influence of the water source on the present virome. By comparing viromes of water and surrounding plants, we observed presence of plant viruses in both compartments, especially in cases of large-scale outbreaks, such as that of tomato mosaic virus. Moreover, we demonstrated that water virome data can extensively inform us about the distribution and diversity of plant viruses for which only limited information is available from plants. Overall, the results of the study provided extensive insights into the virome of irrigation waters from the perspective of plant health. It also suggested that an HTS-based water virome surveillance system could be used to detect potential plant disease outbreaks and to survey the distribution and diversity of plant viruses in the ecosystems.

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Section: 1mentioning

confidence: 99%

Virome analysis of irrigation water sources provides extensive insights into the diversity and distribution of plant viruses in agroecosystems

Ferreira

Bačnik

Rivarez

et al. 2023

Preprint

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“…Geminivirus (single strand DNA viruses) vectors are DNA virus-based vectors which have been recently optimized as multifunctional vectors. 43,44 Generally, the application of virus-based vectors as the basis of transient expression systems enjoy considerable advantages such as speed up of product expression, the decrease of research and development expenses, high expression, flexibility and scalability which are suitable alternatives for protein production instead of permanent expression systems (nucleus and chloroplast). 37 The comparison of the structure and the functional differences between the first and second generation viral vectors is presented in Figure 2 and Table 1.…”

Section: First Generation Virus-based Vectors: Full Virus Strategymentioning

confidence: 99%

Virus-Based Vectors: A New Approach for Production of Recombinant Proteins

Naseri

Khezri

Davarpanah

et al. 2019

J Apple Biotechnol Rep

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Recombinant proteins have become one of the basic human needs today for pharmaceutical, industrial and research purposes. The conventional methods of producing recombinant proteins, including bacterial systems, yeast, and human cells, have led to rising prices and biosafety problems for these products. Research on commercial production of recombinant proteins prompted a new method based on plant RNA viruses and plant hosts, which is known as transient expression system. Accordingly, a viral vector based on the plant RNA virus genome was designed in which the target gene is placed underneath a viral subgenomic promoter. In these vectors, despite the genetic manipulation, the ability to infect the entire plant that was present in the virus was preserved and, several days after plant infestation, it became bioreactor for the production of recombinant protein. The advances made in this field led to the creation of hybrid vectors based on plant viruses and Agrobacterium T-DNA (Transfer DNA). The amount of recombinant protein produced by these vectors was up to 5 g/kg of fresh weight, which is considered a reliable record. The abundant benefits of this method have attracted the attention of researchers. Some of the benefits include: the rapid and high production rate, reduced production costs and the bio-safety of the manufactured products. Several recombinant drugs are currently produced by the transient expression system, either delivered to the consumer or by clinical trials. This review presents how to discover, creation and development of viral vectors in transient expression systems in order to produce recombinant proteins.

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“…Dada su limitada capacidad para expresar proteínas, los virus han desarrollado una serie de estrategias que les capacita para competir con los genes celulares y así pues, lograr una eficiente expresión y replicación del genoma viral (Bustamante and Hull, 1998;Drugeon, 1999;Firth and Brierley, 2012). Entre ellas cabe destacar, (a) la organización multipartita del genoma, (b) la síntesis de sgRNAs, (c) el procesamiento proteolítico de una poliproteína, (d) el solapamiento de pautas abiertas de lectura (open reading frame; ORF), (e) la síntesis alternativa de proteínas de mayor tamaño obviando codones de parada ("read-through") y (f) el desplazamiento de lectura (frameshift), para la síntesis de proteínas de mayor tamaño.…”

Section: Replicación Y Expresión Del Genoma Viralunclassified

Proteínas de movimiento de la familia 30K:interacción con membranas biológicas y factores proteicos y su implicación en el transporte viral

Morell¹

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Proteínas de movimiento de la familia 30K: interacción con membranas biológicas y factores proteicos y su implicación en el transporte viral Memoria presentada por ANA PEIRÓ MORELL para optar al grado de DOCTOR EN BIOTECNOLOGIA Directores Profesor VICENTE PALLÁS BENET Doctor JESÚS ÁNGEL SÁNCHEZ NAVARRO Noviembre 2014 Esta Tesis Doctoral se ha realizado con la financiación del proyecto: "Trafico intracelular, intercelular y vascular de RNAS y proteínas virales y subvirales en plantas' del Ministerio de Ciencia e innovación-Programa Biotecnología (BIO 2011-25018) y con la Beca del Programa JAE-PreDoc del CSIC. ÍNDICE ABREVIATURAS RESUMEN INTRODUCCIÓN GENERAL 1.-EL CONCEPTO DE VIRUS 2.-CLASIFICACIÓN TAXONÓMICA DE LOS VIRUS DE PLANTAS 3.-CICLO INFECTIVO DE UN VIRUS DE PLANTAS 3.1. Entrada y desensamblaje de la cubierta viral 3.2. Expresión y replicación del genoma viral 3.3. Movimiento viral 4.-LAS PROTEÍNAS DE MOVIMIENTO 4.1. Características y clasificación de las MPs 4.2. Modelos de sistemas de transporte célula a célula 4.2.1. Transporte intracelular 4.2.2. Los plasmodesmos 4.2.3. Transporte intercelular -Movimiento basado en la formación de complejos ribonucleoproteicos: el Virus del mosaico del tabaco (TMV) -Movimiento viral guiado por túbulos 4.3. Movimiento sistémico 4.4. Las MPs como determinantes de la especificidad del huésped 5.-LAS MPs COMO DETERMINANTES DE PATOGENICIDAD 6.-GENES DE RESISTENCIA 6.1. Barreras frente a patógenos 6.2. Genes de resistencia -Gen de resistencia Sw-5 7.-TOPOLOGÍA DE LAS PROTEÍNAS DE MEMBRANA 7.1. Clasificación JUSTIFICACIÓN Y OBJETIVOS CAPÍTULO 1The Tobacco mosaic virus movement protein associates with but does not integrate into biological membranes CAPÍTULO2Systemic transport of Alfalfa mosaic virus can be mediated by the movement proteins of several viruses assigned to five genera of the 30K family CAPÍTULO3Patellins 3 and 6, two members of the plant patellin family, interact with the movement protein of Alfalfa mosaic virus and interfere with viral movement CAPÍTULO 4The movement protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance

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Plant virus gene expression strategies

Cited by 4 publications

References 128 publications

Virome analysis of irrigation water sources provides extensive insights into the diversity and distribution of plant viruses in agroecosystems

Virome analysis of irrigation water sources provides extensive insights into the diversity and distribution of plant viruses in agroecosystems

Virus-Based Vectors: A New Approach for Production of Recombinant Proteins

Proteínas de movimiento de la familia 30K:interacción con membranas biológicas y factores proteicos y su implicación en el transporte viral

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