Plant Viruses in Plant Molecular Pharming: Toward the Use of Enveloped Viruses

Ibrahim, Ahmad; Odon, Valerie; Kormelink, Richard

doi:10.3389/fpls.2019.00803

Cited by 45 publications

(32 citation statements)

References 263 publications

(233 reference statements)

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“…The science of plant vectorology has evolved substantially from this first finding. Plant virus expression vectors have been designed from genomes of both positive-sense RNA viruses and single-stranded DNA viruses (Table 2) [10, 16, 47, 62].…”

Section: Main Textmentioning

confidence: 99%

“…In recent decades, plants have been recognized as a possible solution for global health. Plant-made biopharmaceuticals have long been considered a promising technology for providing inexpensive and efficient medicines for LDCs [7–10]. Indeed, plants meet all the criteria for profitable therapeutic and vaccine production, such as efficiency, reduced cost, temperature stability (can be stored at ambient temperatures for prolonged periods of time) and easy transport, even to remote areas [11–13].…”

Section: Introductionmentioning

confidence: 99%

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Virus-based pharmaceutical production in plants: an opportunity to reduce health problems in Africa

Bamogo

Brugidou

Sérémé

et al. 2019

Virol J

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BackgroundDeveloping African countries face health problems that they struggle to solve. The major causes of this situation are high therapeutic and logistical costs. Plant-made therapeutics are easy to produce due to the lack of the safety considerations associated with traditional fermenter-based expression platforms, such as mammalian cells. Plant biosystems are easy to scale up and inexpensive, and they do not require refrigeration or a sophisticated medical infrastructure. These advantages provide an opportunity for plant-made pharmaceuticals to counteract diseases for which medicines were previously inaccessible to people in countries with few resources.Main bodyThe techniques needed for plant-based therapeutic production are currently available. Viral expression vectors based on plant viruses have greatly enhanced plant-made therapeutic production and have been exploited to produce a variety of proteins of industrial, pharmaceutical and agribusiness interest. Some neglected tropical diseases occurring exclusively in the developing world have found solutions through plant bioreactor technology. Plant viral expression vectors have been reported in the production of therapeutics against these diseases occurring exclusively in the third world, and some virus-derived antigens produced in plants exhibit appropriate antigenicity and immunogenicity. However, all advances in the use of plants as bioreactors have been made by companies in Europe and America. The developing world is still far from acquiring this technology, although plant viral expression vectors may provide crucial help to overcome neglected diseases.ConclusionToday, interest in these tools is rising, and viral amplicons made in and for Africa are in progress. This review describes the biotechnological advances in the field of plant bioreactors, highlights factors restricting access to this technology by those who need it most and proposes a solution to overcome these limitations.

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Section: Main Textmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Virus-based pharmaceutical production in plants: an opportunity to reduce health problems in Africa

Bamogo

Brugidou

Sérémé

et al. 2019

Virol J

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“…Infectious clones (or infectious transcripts) of plant viruses remain a basic research tool in plant pathology, concerning mostly studies on virus-derived pathogenicity determinants [ 23 ]. On the other hand, plant virus vectors were described as platforms for heterologous protein production in plants [ 24 ] (revised in [ 25 ]) or for silencing gene expression in hosts (most commonly tobacco rattle virus and potato X virus). The goal of this study was to develop stable ToTV-based constructs suitable for expressing a reporter protein, GFP, in plants.…”

Section: Discussionmentioning

confidence: 99%

Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants

Wieczorek

Budziszewska

Frąckowiak

et al. 2020

Viruses

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Green fluorescent protein (GFP)-tagged viruses are basic research tools widely applied in studies concerning molecular determinants of disease during virus infection. Here, we described a new generation of genetically stable infectious clones of tomato torrado virus isolate Kra (ToTVpJL-Kra) that could infect Nicotiana benthamiana and Solanum lycopersicum. Importantly, a modified variant of the viral RNA2—with inserted sGFP (forming, together with virus RNA1, into ToTVpJL-KraGFP)—was engineered as well. RNA2 of ToTVpJL-KraGFP was modified by introducing an additional open reading frame (ORF) of sGFP flanked with an amino acid-coding sequence corresponding to the putative virus protease recognition site. Our further analysis revealed that sGFP-tagged ToTV-Kra was successfully passaged by mechanical inoculation and spread systemically in plants. Therefore, the clone might be applied in studying the in vivo cellular, tissue, and organ-level localization of ToTV during infection. By performing whole-plant imaging, followed by fluorescence and confocal microscopy, the presence of the ToTVpJL-KraGFP-derived fluorescence signal was confirmed in infected plants. All this information was verified by sGFP-specific immunoprecipitation and western blot analysis. The molecular biology of the torradovirus-plant interaction is still poorly characterized; therefore, the results obtained here opened up new possibilities for further research. The application of sGFP-tagged virus infectious clones and their development method can be used for analyzing plant-virus interactions in a wide context of plant pathology.

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“…The production of recombinant proteins by transgenic plants is highly time-consuming and often results in a low yield (Tschofen et al, 2016). In contrast, a high yield of recombinant proteins can be obtained within 1-2 weeks by a transient gene expression system with a "deconstructed" viral vector system (Ibrahim et al, 2019). The magnICON system, based on the tobacco mosaic virus replication system, is one of the most renowned deconstructed viral vector systems (Gleba et al, 2005;Marillonnet et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

RAP Tag and PMab-2 Antibody: A Tagging System for Detecting and Purifying Proteins in Plant Cells

et al. 2020

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An affinity tag system requires both high affinity and specificity. The RAP tag epitope DMVNPGLEDRIE, derived from rat podoplanin (PDPN), is specifically recognized by PMab-2 monoclonal antibodies in rats. Here, we demonstrated that high levels of PMab-2 can be produced in Nicotiana benthamiana and plant-derived PMab-2 possesses similar activity to CHO-derived PMab-2, and the RAP tag presents a useful tagging system for detecting and purifying proteins from plant cells. The heavy chain of PMab-2 fused with KDEL, an endoplasmic reticulum retention sequence, and the light chain of the antibody were introduced into N. benthamiana by agroinfiltration. The expression of PMab-2 peaked 4 days after agroinfiltration, and approximately 0.3 mg/g fresh weight of the antibody was accumulated. After purification, the plant-derived PMab-2 successfully recognized rat PDPN expressed in CHO-K1 cells and exhibited almost the same binding activity as CHO-derived PMab-2. The RAP-tagged proteins expressed in plant cells were specifically recognized by PMab-2. These results indicate that PMab-2 can accumulate at high levels in N. benthamiana and is easily purified and that the RAP tagging system presents a useful tool for detecting and purifying proteins of interest in plant cells.

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Plant Viruses in Plant Molecular Pharming: Toward the Use of Enveloped Viruses

Cited by 45 publications

References 263 publications

Virus-based pharmaceutical production in plants: an opportunity to reduce health problems in Africa

Virus-based pharmaceutical production in plants: an opportunity to reduce health problems in Africa

Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants

RAP Tag and PMab-2 Antibody: A Tagging System for Detecting and Purifying Proteins in Plant Cells

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