Plant viruses with circular single-stranded DNA

Harrison, B. D.; Barker, H. A.; Bock, K. R.; Guthrie, E. J.; Meredith, Gina; Atkinson, M. R.

doi:10.1038/270760a0

Cited by 173 publications

(86 citation statements)

References 13 publications

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“…Reports (Goodman, 1977;Harrison et al, 1977). The recent description of a dsDNA-containing elongated virus (Sela et aL, 1980) must await confirmation but no doubt new viruses and perhaps new kinds of virus will continue to emerge.…”

Section: Discussionmentioning

confidence: 99%

Guidelines for the Identification and Characterization of Plant Viruses

Hamilton

Edwardson

Francki

et al. 1981

Journal of General Virology

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Section: Discussionmentioning

confidence: 99%

Guidelines for the Identification and Characterization of Plant Viruses

Hamilton

Edwardson

Francki

et al. 1981

Journal of General Virology

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“…BGMV (Haber et al, 1981) and TomGMV (Hamilton et al, 1982(Hamilton et al, , 1983 likewise each possess two single-stranded DNA molecules that differ in nucleotide sequence, but the actual sequences are not yet published. MSV and CSMV also have genomes of circular single-stranded DNA (Harrison et al, 1977;Francki et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

“…The name geminivirus (Harrison et al, 1977;Matthews, 1979) was given to a group of plant viruses that have geminate particles measuring about 30 × 20 nm which contain circular molecules of single-stranded DNA, and that are transmitted by species of leafhoppers or whiteflies. In early work, no serological relationship was detected between three geminiviruses, maize streak virus (MSV), African cassava mosaic virus (ACMV = cassava latent virus; Bock & Woods, 1983), and chloris striate mosaic virus (CSMV) : Francki et al, 1979.…”

Section: Introductionmentioning

confidence: 99%

Serological Relationships and Genome Homologies among Geminiviruses

Roberts¹,

Robinson²,

Harrison³

1984

Journal of General Virology

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SUMMARYIn immunosorbent electron microscopy (ISEM) tests, strong relationships were detected between five whitefly-transmitted geminiviruses: African cassava mosaic (ACMV), bean golden mosaic, euphorbia mosaic, squash leaf curl and tomato golden mosaic. Among five leafhopper-transmitted geminiviruses, beet curly top and tobacco yellow dwarf viruses were distantly related but no relationship was detected between either chloris striate mosaic, maize streak or wheat dwarf viruses and any of the other four. No relationship was detected between any whitefly-transmitted and any leafhopper-transmitted virus. A similar pattern of relationships was found by spot hybridization experiments in which extracts from infected leaves were tested with probes for ACMV DNA-1 or DNA-2. Imperfect nucleotide sequence homologies were found between ACMV DNA-1, which contains the particle protein gene, and the DNA of five other whitefly-transmitted viruses: bean golden mosaic, tomato golden mosaic, tobacco leaf curl, tomato leaf curl and tomato yellow leaf curl, the last three of which are not sap-transmissible. Thus, relationships were established between saptransmissible and sap non-transmissible geminiviruses. No homologies were detected with a full-length probe for ACMV DNA-2. Extracts from plants infected with three teafhopper-transmitted viruses (beet curly top, maize streak and wheat dwarf) did not react with probes for ACMV DNA-1 or DNA-2. Because each of the leafhoppertransmitted geminiviruses has a different vector species whereas the whiteflytransmitted geminiviruses all have the same vector, Bemisia tabaci, the genome homologies and antigenic relationships detected among members of the group could be explained if their coat proteins have a key role in transmission by vectors.

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“…The Digitaria geminivirus DNA gave two closely spaced major bands. If this DNA behaves as ACMV DNA, the slower band corresponds to the circular form, and the faster one to linear molecules (Harrison et al, 1977). The linear DNA was estimated to consist of 2350 (_+ 20) nucleotides.…”

mentioning

confidence: 99%

A Geminivirus, Serologically Related to Maize Streak Virus, from Digitaria sanguinalis from Vanuatu

Dollet¹,

Accotto

Vaccari

et al. 1986

Journal of General Virology

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SUMMARYElectron microscopy of purified particles of a virus found in Dig#aria sanguinalis from Vanuatu (formerly New Hebrides) indicated that it is a geminivirus. Preparations of virus particles contained one coat protein of tool. wt. about 27 500 and circular and linear single-stranded DNA about 2350 nucleotides in length. In thin sections of infected cells, geminate particles were found in crystalline arrays both in nuclei and in the cytoplasm. The virus is serologically related to maize streak virus but differs from it with a serological differentiation index of 3.A striate mosaic disease of Digitaria sanguinatis in Vanuatu (formerly New Hebrides) was described some years ago (Dollet & De Taffin, 1979), but the causal agent was not identified. In this paper, we show that the diseased plants contain geminate virus particles (Francki et al., 1985; Harrison, 1985) that are serologically related to maize streak virus (MSV) (Bock et al., 1974).Infected D. sanguinalis plants were collected in the field and vegetatively propagated in an insect-proof glasshouse. Healthy D. sanguinalis was grown from seed in sterilized soil in the same glasshouse.Pieces of diseased leaf were fixed in glutaraldehyde and osmium tetroxide and embedded in epoxy resin (Dollet et al., 1983). Sections were stained in uranyl acetate and lead citrate. Linear aggregates of geminate particles (16 to 21 nm) were observed in the nuclei of companion and phloem parenchyma cells, often in large amounts (Fig. 1). Similar arrays of particles were also found in intact areas of cytoplasm, in cells in which nuclei appeared to be intact. Among other geminiviruses, this unusual location has been reported only for chloris striate mosaic virus (CSMV) (Francki et al., 1979). Virus particles were purified by a method derived from that of Larsen & Duffus (1984). Frozen leaves were ground in 0-1 M-potassium phosphate buffer pH 7, containing 10 mM-EDTA, 10 mM-sodium diethyldithiocarbamate and 20 mM-Na2SO3. The sap was treated with 1% Triton X-100 and clarified with 1/10 vol. chloroform/butanol (1:1, v/v). Virus particles were concentrated with 12% polyethylene glycol and 0.2 M-NaC1 and a cycle of differential centrifugation. The pellets were dissolved in 0.1 M-phosphate buffer pH 7 containing 10 mM-EDTA, and the virus preparation ultracentrifuged in a 10 to 40% sucrose density gradient prepared in the same buffer. The virus-containing band was concentrated by ultracentrifugation, and the sucrose density gradient centrifugation was then repeated. The yield was about 6 mg of virus per kg of fresh leaf tissue (a relatively large amount for a geminivirus), estimated assuming that Almg/ml is 7"7 (Goodman & Bird, 1978). Most particles in the purified virus ~ ~260nm, I cm preparations were geminate (Fig. 2), and the remaining few were single. The methods of 0000-6920 © 1986 SGM

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Plant viruses with circular single-stranded DNA

Cited by 173 publications

References 13 publications

Guidelines for the Identification and Characterization of Plant Viruses

Guidelines for the Identification and Characterization of Plant Viruses

Serological Relationships and Genome Homologies among Geminiviruses

A Geminivirus, Serologically Related to Maize Streak Virus, from Digitaria sanguinalis from Vanuatu

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