In Plantago lanceolata 14 out of 39 enzyme loci showed genetic variation, as checked by analysis of crosses. Five linkage groups could be formed with these variable loci. They may represent five different chromosomes as n =6 in this species. The SI locus, controlling self-incompatibility, appeared to be very tightly linked with Pgm-1 (recombination = 08 per cent) and somewhat more loosely with 6Pgd-1 and 6Pgd-2. In this linkage group also a recessive male sterility locus could be placed. A different male sterility locus with recessive effect was located in a second linkage group, and a male sterility locus with dominant effect in a third one.These results, and the finding of differences in germination behaviour when certain chromosomes are substituted, point to the possible further use of allozyme loci in analysing the genetic basis of ecologically important differences in P. lanceolata.