2006
DOI: 10.1080/02648725.2006.10648087
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Plantibodies: A Novel Strategy to Create Pathogen-Resistant Plants

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Cited by 5 publications
(2 citation statements)
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“…In addition they carry two heavy chains and two light chains, and thus it is difficult to genetically manipulate them to construct fusion proteins with other partners. Rapid progress in molecular immunology, combined with the polymerase chain reaction, has made it possible to clone the antibody binding domain (Fv fragment) and express the polypeptide chains in bacteria, yeast, mammalian cells and plant cells either as pure antibodies or as fusion proteins comprising antibodies genetically linked to other peptides [ 29 , 30 ]. By advanced technologies such as phage display, antibody fragments specific for particular antigens can be isolated in vitro from libraries containing diverse repertoires of antibodies V-genes, which bypasses hybridoma technology altogether and generates single-chain antibodies with specificity and affinity similar to monoclonal antibodies.…”
Section: Antibodies Specific For Fusarium Mycotmentioning
confidence: 99%
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“…In addition they carry two heavy chains and two light chains, and thus it is difficult to genetically manipulate them to construct fusion proteins with other partners. Rapid progress in molecular immunology, combined with the polymerase chain reaction, has made it possible to clone the antibody binding domain (Fv fragment) and express the polypeptide chains in bacteria, yeast, mammalian cells and plant cells either as pure antibodies or as fusion proteins comprising antibodies genetically linked to other peptides [ 29 , 30 ]. By advanced technologies such as phage display, antibody fragments specific for particular antigens can be isolated in vitro from libraries containing diverse repertoires of antibodies V-genes, which bypasses hybridoma technology altogether and generates single-chain antibodies with specificity and affinity similar to monoclonal antibodies.…”
Section: Antibodies Specific For Fusarium Mycotmentioning
confidence: 99%
“…Expression levels observed in the transient assay are very consistent with those obtained with transgenic approach. We routinely use this system to test expression of antibodies or genes of interest, and to study their functions before moving to generating transgenic plants [ 29 , 55 ].…”
Section: Antibody Expression In Plantsmentioning
confidence: 99%