Plantlet regeneration through somatic embryogenesis in Nordmann’s fir (Abies nordmanniana)

Nawrot-Chorabik, Katarzyna

doi:10.1007/s11676-016-0265-7

Cited by 11 publications

(10 citation statements)

References 17 publications

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“…In the genus Abies somatic embryogenesis has been initiated in several species, including A. alba (Schuller et al, 1989; Zoglauer and Reuther, 1996; Nawrot-Chorabik, 2008; Vooková and Kormutak, 2009), A. balsamea (Guevin et al, 1994), A. cephalonica (Krajňáková et al, 2008), A. fraseri (Guevin and Kirby, 1997; Pullmann et al, 2016), A. lasiocarpa (Kvaalen et al, 2005), A. nordmanniana (Nörgaard and Krogstrup, 1991; Misson et al, 2006; Nawrot-Chorabik, 2016), A. numidica (Vooková et al, 2001, Vooková et al, 2003), A. cilicica (Vooková and Kormut’ák, 2003), and hybrid firs (Gajdošová et al, 1995; 1997/1998; Korecký and Vitámvás, 2011). It is worthy of mention that in A. alba somatic embryogenesis was initiated as early as 1986 (Erdelský and Barančok, 1986).…”

Section: Somatic Embryogenesis In the Hybrids Abies Alba X A Cephalomentioning

confidence: 99%

Somatic Embryogenesis in Selected Conifer Trees Pinus nigra Arn. and Abies Hybrids

et al. 2019

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Somatic embryogenesis was achieved in the conifers Pinus nigra Arn. and in the hybrids Abies alba ×A. cephalonica and Abies alba ×A. numidica. For initiation of embryogenic tissue in P. nigra, immature zygotic embryos enclosed in megagametophytes were used. The initiated embryogenic cultures were maintained and proliferated on solid culture medium DCR supplemented with 9 μM 2,4-D and 2.2 μM BA. Microscopic investigations revealed the presence of bipolar early somatic embryos in proliferating tissue. Suspension cultures have also been established by resuspending the embryogenic tissue in liquid culture medium. Experimentation with abscisic acid concentration resulted in successful somatic embryo maturation. Besides abscisic acid, the carbohydrate content or higher concentration of gelling agent in maturation medium were also important requirements for somatic embryo maturation. Germination of cotyledonary somatic embryos occurred on hormone-free medium and terminated in somatic seedlings regeneration. The regenerated somatic seedlings were transferred to soil and were capable of successful development. For initiation of embryogenic tissue in Abies hybrids juvenile explants as immature or mature zygotic embryos as well as cotyledons were used and 4.4 μM BA as sole plant growth regulator was sufficient. Medium of the same composition was also suitable for their long-term maintenance. Maturation of somatic embryos was achieved on solid DCR medium supplemented with 38 μM abscisic acid, polyethylene glycol (0, 5, 7.5, and 10% PEG-4000) and different carbohydrates such as maltose, sucrose and glucose (each 3%). PEG-4000 stimulated somatic embryo development depending on the carbohydrate source used. Cotyledonary somatic embryos germinated after desiccation treatment and the regenerated somatic seedlings were transferred to soil. Cryopreservation of embryogenic tissue could be an alternative method for long-term maintenance. For cryopreservation the slow-freezing method was used with success. Tissue regeneration in the post thaw period was relatively high and the regenerated tissue produced mature somatic embryos and subsequent plantlets. The embryogenic tissue was also used in experiments focused on genetic transformation either by biolistic (P. nigra) or Agrobacterium-mediated (Abies hybrids) methods. A proteomic study was performed to gain a deeper insight into the early stages of P. nigra somatic embryogenesis.

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Section: Somatic Embryogenesis In the Hybrids Abies Alba X A Cephalomentioning

confidence: 99%

Somatic Embryogenesis in Selected Conifer Trees Pinus nigra Arn. and Abies Hybrids

et al. 2019

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“…Abies nordmanniana is a decorative species widely used in Europe for the production of "Christmas trees", which are sold annually in the amount of about 42 million trees [14]. Accordingly, a significant part of Abies nordmanniana studies is aimed at reproduction of the species and preservation of decorative effect of trees in culture [14,[15][16][17][18]. The literature resources present few data on the species in the natural conditions of the native range limited to the territory of the Russian Caucasus, Georgia and the north-eastern regions of Turkey [19].…”

Section: Studied Speciesmentioning

confidence: 99%

Differentiation of ecological niches of the forest-forming species in the Caucasus

et al. 2021

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Adaptations of Abies nordmanniana, Picea orientalis, Pinus sylvestris, Fagus orientalis, and Carpinus betulus to the abiotic environmental conditions of the study area largely determined their predicted distribution in the Caucasus. The ecological niches of the species mostly coincided in two analyzed complex environmental factors (characteristics of water regime and topographic parameters). The complex humidity factor was the main factor determining the potential distribution the forest-forming species in the Caucasus (65% of the contribution in the Maxent models). Topographic ENVIREM predictors were also significant in the species distribution (20% of the contribution in the models). Abies nordmanniana and Fagus orientalis were the most sensitive to the humidity factor, while Pinus sylvestris depended largely on the topographic factors. The similarity of the distribution potential of the studied species in the Caucasus was explained largely by a high degree of overlap of ecological niches (Schoener’s D = 0.55-0.79) and their visual overlap in the orthogonal space of the analyzed ecological factors. The largest Schoener’s D indexes were observed for the pairs Pinus sylvestris – Picea orientalis, Abies nordmanniana – Picea orientalis, Fagus orientalis – Picea orientalis, and Fagus orientalis – Carpinus betulus. Carpinus betulus, Fagus orientalis and Pinus sylvestris had the widest ecological niches.

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“…The entire process can require up to 2 days. For somatic embryogenesis of species of Abies, Pinus and Picea (Nawrot-Chorabik 2008, 2016Nawrot-Chorabik et al 2011, 2015a, seeds are precooled in a solution of antioxidants (5.0% v/v ascorbic acid or polyvinylpyrrolidone in sterile water), for 24 h in 4°C. This application of antioxidants is an innovative step, facilitating isolation of somatic embryos and inhibiting secretion of phenolic glycosides in seeds that interfere with callogenesis (Nawrot-Chorabik 2016).…”

Section: Plant Materials and General Sterilization Guidelinesmentioning

confidence: 99%

“…The culture medium for each stage of in vitro somatic embryogenesis must be modified and optimized for the intended result, mainly by altering the types and concentrations of phytohormones, carbohydrates and other components. Media for arborescent plants are rich in macroand microelements, vitamins, growth regulatory hormones and, in some cases, the source of amino acids (enzymatic casein hydrolysate), as recommended for species of Abies (Nawrot-Chorabik 2008, 2016. The pH of media for woody species ranges from 5.7 to 5.8.…”

Section: Culture Media Compositionmentioning

confidence: 99%

“…Since the second half of the 1980s, new biotechnological research directions have been applied worldwide in forestry and led to numerous specialized methods, including in vitro tissue culture via somatic embryogenesis, designed especially for gymnosperm species (Litz et al 1995;Giri et al 2004), and organogenesis for gymnosperms and angiosperms (Rangaswamy 1986). Somatic embryogenesis results in a potentially high efficacy of micropropagation by yielding valuable, highly selected plant reproductive material (Rodríguez et al 2007), enabling research on pathogenicity at the embryonic stage of plant development (using callus tissue) (Hendry et al 1993;Nawrot-Chorabik 2014;Nawrot-Chorabik et al 2011, 2016, production of secondary metabolites (Mulabagal and Tsay 2004), transformation of plants (Walters et al 2005), and cryopreservation of plant material (Misson et al 2006;Hargreaves and Menzies 2007;Nawrot-Chorabik and Sitko 2014). These broad applications explain the increasing interest in somatic embryogenesis and its indirect product, callus tissue.…”

Section: Introductionmentioning

confidence: 99%

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Ecophysiological aspects of in vitro biotechnological studies using somatic embryogenesis of callus tissue toward protecting forest ecosystems

Nawrot-Chorabik¹,

Pietrzykowski

2018

J. For. Res.

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This review on current biotechnological methods in forestry for in vitro tissue cultures to define the effect of stress conditions on trees, concentrates on somatic embryogenesis. Callus tissue, the key product of somatic embryogenesis, grows over a tree wound under ex vitro conditions. Callus tissue can be used in research in areas such as pathogenic susceptibility at the embryonic level, effect of heavy metals, influence of low temperatures (cryopreservation), production of secondary metabolites and transformation of plants. Callus of arborescent plants can be induced in vitro by fungal elicitors to produce secondary metabolites for pharmaceutical and cosmetic industries and are strongly repellant to herbivores and can thus act to protect forests. Analyses of dual cultures demonstrated that callus tissue exposed to a pathogenic fungus responds by synthesizing low-molecular-mass proteins belonging to an immune protein class. Cryopreservation of embryonic callus tissue also has broad applications, e.g., for valuable plant genotypes in gene banks. Without strategies to protect forests against stress factors, forest ecosystems will degrade to the detriment of all life, including humans. In vitro biotechnological research using callus tissue contributes to progress in forestry and the disciplines of ecology, physiology, phytopathology, culture and selection of plants.

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Plantlet regeneration through somatic embryogenesis in Nordmann’s fir (Abies nordmanniana)

Cited by 11 publications

References 17 publications

Somatic Embryogenesis in Selected Conifer Trees Pinus nigra Arn. and Abies Hybrids

Somatic Embryogenesis in Selected Conifer Trees Pinus nigra Arn. and Abies Hybrids

Differentiation of ecological niches of the forest-forming species in the Caucasus

Ecophysiological aspects of in vitro biotechnological studies using somatic embryogenesis of callus tissue toward protecting forest ecosystems

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