Plants growing in Colombia as sources of active ingredients for sunscreens

Fuentes, Jorge Luı́s; Mantilla, Diego Armando Villamizar; González, Silvia Juliana Flores; Núñez, Luis A.; Stashenko, Elena

doi:10.1080/09553002.2021.1987564

Cited by 6 publications

(10 citation statements)

References 66 publications

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“…This indicates that they acted as filters that absorbed or refracted the UV rays and reduced genotoxicity. As in the previous studies [ 17 , 31 , 32 , 33 ], our data support the use of DNA damage detection assay (in this case, the SOS Chromotest) as an effective complement that improves the efficacy of photoprotection measurement.…”

Section: Discussionsupporting

confidence: 89%

“…It has been indicated that active ingredients of commercial sunscreens can be toxic to humans and coral reefs [ 8 , 9 , 10 , 11 ], which has increased the interest in using phytochemicals in sunscreen formulations [ 12 , 13 , 14 , 15 , 16 , 17 ]. Phytochemicals are genoprotective and anticancer agents [ 18 , 19 ] that are considered to be non-toxic and pharmacologically safe for human consumption [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Flower Extracts from Ornamental Plants as Sources of Sunscreen Ingredients: Determination by In Vitro Methods of Photoprotective Efficacy, Antigenotoxicity and Safety

et al. 2022

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Plants are sources of sunscreen ingredients that prevent cellular mutations involved in skin cancer and aging. This study investigated the sunscreen properties of the extracts from some ornamental plants growing in Colombia. The UV filter capability of the flower extracts obtained from Rosa centifolia L., Posoqueria latifolia (Rudge) Schult, and Ipomoea horsfalliae Hook. was examined. Photoprotection efficacies were evaluated using in vitro indices such as sun protection factor and critical wavelength. UVB antigenotoxicity estimates measured with the SOS Chromotest were also obtained. Extract cytotoxicity and genotoxicity were studied in human fibroblasts using the trypan blue exclusion and Comet assays, respectively. Major compounds of the promising flower extracts were identified by UHPLC–ESI+–Orbitrap–MS. The studied extracts showed high photoprotection efficacy and antigenotoxicity against UVB radiation, but only the P. latifolia extract showed broad-spectrum photoprotection at non-cytotoxic concentrations. The P. latifolia extract appeared to be safer for human fibroblast cells and the R. centifolia extract was shown to be moderately cytotoxic and genotoxic at the highest assayed concentrations. The I. horsfalliae extract was unequivocally cytotoxic and genotoxic. The major constituents of the promising extracts were as follows: chlorogenic acid, ecdysterone 20E, rhamnetin-rutinoside, cis-resveratrol-diglucoside, trans-resveratrol-diglucoside in P. latifolia; quercetin, quercetin-glucoside, quercetin-3-rhamnoside, kaempferol, kaempferol-3-glucoside, and kaempferol-rhamnoside in R. centifolia. The potential of the ornamental plants as sources of sunscreen ingredients was discussed.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Flower Extracts from Ornamental Plants as Sources of Sunscreen Ingredients: Determination by In Vitro Methods of Photoprotective Efficacy, Antigenotoxicity and Safety

et al. 2022

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“…14 The use of a high-throughput platform (96-well microplate) has notably increased the overall capacity, reproducibility, and robustness of the Comet assay. [15][16][17][18] We recently showed that different plant extracts and major compounds have UV-filter/antigenotoxic properties 19 ; therefore, they could potentially be used as cosmetic sunscreen ingredients. We hypothesize that these plant extracts and their constituents are neither cytotoxic nor genotoxic in human fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

“…Relevant Information Available in the Literature on Activities of the Studied Plants.In vitro photoprotection/antigenotoxicity against UVB radiation19 In vitro cytotoxicity in human fibroblast cells [19 , this work] In vitro genotoxicity in human fibroblast cells [this work]In vitro cytotoxicity in human breast and cervical cancer cell lines[28] In vitro photoprotection/antigenotoxicity against UVB radiation19 In vitro cytotoxicity in human fibroblast cells [19 , this work] In vitro genotoxicity in human fibroblast cells [this work]In vitro photoprotection/antigenotoxicity against UVB radiation19 In vitro cytotoxicity in human fibroblast cells [19 , this work] In vitro genotoxicity in human fibroblast cells [this work]In vitro cytotoxicity in Trypanosoma cruzi cells34 Acute toxicity activity against insects[35][36][37] Acute toxicity activity against Artemia franciscana38 In vitro photoprotection/antigenotoxicity against UVB radiation19 In vitro cytotoxicity in human fibroblast cells [19 , this work] In vitro genotoxicity in human fibroblast cells [this work]…”

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confidence: 99%

In vitro Safety Assessment of Extracts and Compounds From Plants as Sunscreen Ingredients

Flórez González,

Stashenko,

Ocazionez

et al. 2024

Int J Toxicol

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This work investigated the safety of extracts obtained from plants growing in Colombia, which have previously shown UV-filter/antigenotoxic properties. The compounds in plant extracts obtained by the supercritical fluid (CO2) extraction method were identified using gas chromatography coupled to mass spectrometry (GC/MS) analysis. Cytotoxicity measured as cytotoxic concentration 50% (CC50) and genotoxicity of the plant extracts and some compounds were studied in human fibroblasts using the trypan blue exclusion assay and the Comet assay, respectively. The extracts from Pipper eriopodon and Salvia aratocensis species and the compound trans-β-caryophyllene were clearly cytotoxic to human fibroblasts. Conversely, Achyrocline satureioides, Chromolaena pellia, and Lippia origanoides extracts were relatively less cytotoxic with CC50 values of 173, 184, and 89 μg/mL, respectively. The C. pellia and L. origanoides extracts produced some degree of DNA breaks at cytotoxic concentrations. The cytotoxicity of the studied compounds was as follows, with lower CC50 values representing the most cytotoxic compounds: resveratrol (91 μM) > pinocembrin (144 μM) > quercetin (222 μM) > titanium dioxide (704 μM). Quercetin was unique among the compounds assayed in being genotoxic to human fibroblasts. Our work indicates that phytochemicals can be cytotoxic and genotoxic, demonstrating the need to establish safe concentrations of these extracts for their potential use in cosmetics.

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“…Many aromatic species from the Asteraceae, Annonaceae, Lamiaceae, Poaceae, Rutaceae, Verbenaceae, and Zingiberaceae families grown in Colombia have important potential for the discovery of biologically active substances [1,2], because they produce endogenous antioxidants necessary for the neutralization of reactive oxygen species (ROS), including phenolics, flavonoids, anthocyanidins and anthocyanins, phenylpropanoids, stilbenes, terpenes and terpenoids, steroids, lignans, carotenoids, and essential oils (EOs) [3][4][5]. All these compounds play a significant role as defense mechanisms against infections and as a means of survival in an adverse environmental climate, e.g., in an environment rich in solar radiation [6].…”

Section: Introductionmentioning

confidence: 99%

Photoprotective Agents Obtained from Aromatic Plants Grown in Colombia: Total Phenolic Content, Antioxidant Activity, and Assessment of Cytotoxic Potential in Cancer Cell Lines of Cymbopogon flexuosus L. and Tagetes lucida Cav. Essential Oils

Caballero-Gallardo

Quintero-Rincón

Stashenko

et al. 2022

Plants

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Photoprotective agents obtained from plants provide benefits for the health of the skin. The present study aims to assess the total phenolic content (TPC) and in vitro UV-protective properties of twelve essential oils (EOs) from plants grown in Colombia and to evaluate the antioxidant and cytotoxic potential of two species identified as photoprotective potentials: Cymbopogon flexuosus and Tagetes lucida. The composition of EOs was studied by GC/MS. The cytotoxicity of both EOs was examined using an MTT assay, and an H2-DCFDA probe was employed to estimate the intracellular production of ROS in HepG2 and Calu-1 cells. Major constituents (≥10%) were neral, geranial, geranyl acetate in C. flexuosus and estragole in T. lucida. The TPC for C. flexuosus and T. lucida EOs were ≥10 mg GAE/g byproduct. Both EOs showed photoprotective properties (SPFin vitro: 13–14), and long-wavelength UVA protection (λc > 370 nm). HepG2 and Calu-1 cells exposed to C. flexuosus exhibited antiproliferative activity (˂50%) at 125 µg/mL, while T. lucida was at 250 and 500 µg/mL. The IC50 values for C. flexuosus were 75 and 100 µg/mL in HepG2 and Calu-1 cells, respectively, whereas those for T. lucida were >250 µg/mL. These EOs achieved significant inhibitory effects (between 15.6 and 40.4%) against H2O2-induced oxidative stress. The results showed that EO compounds recognized as antioxidants could counteract the effects elicited by H2O2.

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Plants growing in Colombia as sources of active ingredients for sunscreens

Cited by 6 publications

References 66 publications

Flower Extracts from Ornamental Plants as Sources of Sunscreen Ingredients: Determination by In Vitro Methods of Photoprotective Efficacy, Antigenotoxicity and Safety

Flower Extracts from Ornamental Plants as Sources of Sunscreen Ingredients: Determination by In Vitro Methods of Photoprotective Efficacy, Antigenotoxicity and Safety

In vitro Safety Assessment of Extracts and Compounds From Plants as Sunscreen Ingredients

Photoprotective Agents Obtained from Aromatic Plants Grown in Colombia: Total Phenolic Content, Antioxidant Activity, and Assessment of Cytotoxic Potential in Cancer Cell Lines of Cymbopogon flexuosus L. and Tagetes lucida Cav. Essential Oils

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