Plaque Assay of Herpesvirus Hominis on Human Embryonic Fibroblasts

Wentworth, Berttina B.; French, Linda

doi:10.3181/00379727-131-33932

Cited by 35 publications

(18 citation statements)

References 4 publications

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“…HSV-HF, as a representative of HSV type 1, was a gift from Dr. S. Nii. HSV-UW268, as a representative of HSV type 2, was donated by Dr. K. Hayashi [Wentworth and French, 1969].…”

Section: Materials and Methods Cell Lines And Virusesmentioning

confidence: 99%

Effect of herpes simplex virus on the DNA of human papillomavirus 18

et al. 1997

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Some types of human papillomaviruses (HPV) appear to be associated with carcinoma of the cervix or other tissues, but patients infected with HPV do not necessarily develop carcinoma. Some epidemiological studies of risk factors for cervical carcinoma have indicated the involvement of herpes simplex virus (HSV). To study the effect of HSV on the genome of HPV, total DNAs were extracted and analyzed after HeLa cells, or A431 cells, transiently transfected with HPV18 DNA, were infected with HSV-1 or -2 for 24 hours. In HeLa cells, integrated HPV18 DNA was amplified almost threefold. In A431 cells, HPV 18 DNA fragments, sensitive to the restriction enzyme Mbo I, indicated newly replicated DNA. Replication intermediates were detected when the DNA was resolved by two-dimensional gel electrophoresis. This study showed that HSV caused some amplification of HPV and indicated the possibility of HSV involved in the integration and amplification of HPV in host cells.

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“…HSV-HF, as a representative of HSV type 1, was a gift from Dr. S. Nii. HSV-UW268, as a representative of HSV type 2, was donated by Dr. K. Hayashi [Wentworth and French, 1969].…”

Section: Materials and Methods Cell Lines And Virusesmentioning

confidence: 99%

Effect of herpes simplex virus on the DNA of human papillomavirus 18

et al. 1997

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“…were used throughout. Viral titers were deter mined by plaque assay under agarose overlays [13,14]. For HSV, monolayers in 60-mm Petri dishes were chilled to 4 and exposed to virus at an input multiplic ity of approximately 5 PFU/cell.…”

Section: Virusmentioning

confidence: 99%

Effects of Canavanine Treatment on Herpesvirus Morphogenesis in Cultured Cells

Smith¹,

Moore²

1981

Intervirology

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L-Canavanine, a naturally occurring analog of arginine, effectively inhibited the morphogenesis of herpes simplex virus (HSV) and human cytomegalovirus (HCMV) when added at the time of infection, but allowed the expression of several cytopathic changes. Exposure to canavanine at progressively later times ultimately led to qualitatively normal virion maturation. Under no conditions were morphologically aberrant viral particles observed. However, HSV-infected cells treated at 3 h postinfection or later did contain distinctive cytoplasmic inclusions resembling HCMV dense bodies. Recovery experiments showed that HCMV-infected cells exposed to canavanine for 1–5 days could support normal viral morphogenesis when washed free of this agent.

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“…B o t h strains were propagated on monolayers of 13HK-21 cells. Virus pools were titrated as plaque forming units (PFU) on WI-38 cells by the described procedure (19). Cytomegalovirus (CMV) strain AD 169, purchased from Flow laboratories, was grown on WI-38 cells for quantitation by the plaque assay.…”

Section: Organismsmentioning

confidence: 99%

In vitro acquisition of resistance against herpes simplex virus by permissive murine macrophages

Sethi¹,

Brandis²

1979

Archives of Virology

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SummaryThis study has documented that peritoneal macrophages (PM) from adult mice of strains DBA/2J, BALB/c and AKR can support productive replication of herpes simplex virus (ItSV) strains Thea and Haase. Simian virus 40 transformed PlY[ of C57 BL/6J origin yielded high titers of Thea and Haase, whereas normal PM from adult mice of the same genetic background were infected abortively. The evidence obtained suggested that multiplication of PM per se is insufficient to allow ttSV replication. PI~ from DBA/2J mice, immune to ttSV or Listeria monocytogenes lacked the capacity to support productive replication of HSV. PM from nonim_mune, adult DBA/2J or suckling C57BL/6J mice acquired the potential to effectively restrict HSV replication when preineubated for 24 hours with 72 hours old MLC supernatant. These macrophages could also inhibit HSV replication if pretreated with supernatant from cultures of specific antigen stimulated, HSV or Listeria immune spleen lymphocytes (SL). Likewise, supernatant from concanavalin A stimulated SL also possessed the capacity to confer resistance against HSV replication in otherwise permissive PM. Sensitized T cells proved to be essential for the generation of active supernatants. Active supern~tants had the potential to confer resistance against HSV replication within mouse but not chick or hamster embryo cells. The other characteristics of the soluble mediator included: instability at pH 2, inactivation by trypsin but not by DNaso or RNase and persistence of activity when exposed to 56 ° C for 20 minutes. IntroduetionA number of cell mediated mechanisms which depend upon the immune status of the host have been implicated in the acquired immunity against herpes simplex virus (HSV) infections (1--9). Data from in vivo studies carried out by Allison's 11 Arch. ¥irol. 59/3

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Plaque Assay of Herpesvirus Hominis on Human Embryonic Fibroblasts

Cited by 35 publications

References 4 publications

Effect of herpes simplex virus on the DNA of human papillomavirus 18

Effect of herpes simplex virus on the DNA of human papillomavirus 18

Effects of Canavanine Treatment on Herpesvirus Morphogenesis in Cultured Cells

In vitro acquisition of resistance against herpes simplex virus by permissive murine macrophages

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