Plasma-activated immobilization of biomolecules onto graphite-encapsulated magnetic nanoparticles

Saraswati, Teguh Endah; Oginö, Kazuhíde; Nagatsu, Masaaki

doi:10.1016/j.carbon.2011.10.044

Cited by 51 publications

(32 citation statements)

References 26 publications

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“…Sintesis nanopartikel magnetik besi dengan karbon telah dilaporkan oleh Saraswati et al, 2011;Saraswati et al, 2012; dengan metode arc discharge konvensional. Metode arc discharge atau metode pijar api adalah salah satu metode top-down untuk membuat nanomaterial.…”

Section: Pendahuluanunclassified

Surface Character of Iron Oxide/Carbon Nanoparticles Synthesized by Submerged Arc Discharge Method in Ethanol/Urea Medium

2017

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Synthesis of iron oxide nanoparticles modified with carbon has been successfully performed by submerged arc-discharge method in ethanol/urea medium. Iron oxide used in the fabrication process was prepared by iron electrolysis in an electrolyte solution of NaCl. Fabrication of nanoparticles in this method uses two graphite electrodes. One of them was made in a pointed shape and the other graphite electrodes hollowed out and filled with a mixture of iron oxide, graphite and glue silica (as binder) with a ratio of 1:3:1 (w/w/w). The liquid medium used in this method is a mixture solution of ethanol 50% and urea (0%, 10%, 25% and 50%) with a volume ratio of 1:1 (v/v). The crystalline of iron oxide was characterized using X-Ray Diffraction (XRD), compared to JCPDS No. 89-0597, No. 89-0691 and No. 39-1346. Variations in the urea concentration in the liquid medium provided the changes of the surface character of the synthesized nanoparticles. The changes of surface character were analyzed by the Fourier Transform Infra Red (FTIR) spectra and nanoparticle dispersion in water and ethanol. FTIR spectra showed the absorption of Fe-O, CH, CN, C = O, OH and NH at 460-555 cm-1, 650-1000 cm-1, 1000-1350 cm-1, 1640-1680 cm-1, 2400-3400 cm-1, 3200-3400 cm-1, 3100-3500 cm-1, respectively. The best hydrophilic surface character achieved when the nanoparticle was synthesized in medium of ethanol 50% with the addition of urea50%. The existence of a functional group attached on the surface of nanoparticles synthesized in ethanol/urea makes these nanoparticles had better dispersion than nanoparticles synthesized in ethanol medium without urea addition.

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Section: Pendahuluanunclassified

Surface Character of Iron Oxide/Carbon Nanoparticles Synthesized by Submerged Arc Discharge Method in Ethanol/Urea Medium

2017

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“…18,19 Previously, we have shown that the surface of MNBs can be modified by the introduction of amino groups using radio frequency (RF) ammonia plasma. 20 The modified surface of the MNBs can then readily incorporate specific antibodies against a pathogen such as Salmonella, 21 dengue virus, 22 influenza virus, 23 and Escherichia coli. 24 Our results show that MNBs decorated with antibodies can facilitate the concentration of these pathogens in environmental samples.…”

Section: Introductionmentioning

confidence: 99%

Efficient recovery and enrichment of infectious rotavirus using separation with antibody-integrated graphite-encapsulated magnetic nanobeads produced by argon/ammonia gas plasma technology

Yamashiro

Sakudo

Nagatsu

2019

IJN

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Background: Rotavirus is the representative cause of severe acute gastroenteritis in young children. A characteristic feature of rotavirus is low infectious dose and robustness of the virion, suggesting sanitation and hygiene will have little impact. Thus, development of a vaccine should be given priority. Efficient capture of infectious viruses is an important step in generating a vaccine. Previously, antibody-integrated magnetic nanobeads (MNBs) have been developed for virus capture. This study examines the applicability of this method for infectious rotavirus recovery and enrichment. Materials and methods: Graphite-encapsulated MNBs were treated with radio frequency (RF) excited Ar/NH 3 gas mixture plasma to introduce amino groups onto their surfaces. Rotavirus viral protein 7 (VP7) antibody was attached to the surface of MNBs via these amino groups using a coupling agent, N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP). The antibodyintegrated MNBs were incubated with rotavirus-infected cell lysate and then separated from the supernatant by applying a magnetic field. After thorough washing, rotavirus was recovered and enrichment analysis done by polymerase chain reaction (PCR), immunochromatography, and an infection analysis using MA104 cells. Results and discussion: Immunochromatography and PCR indicate that anti-rotavirus antibody-integrated MNPs efficiently capture rotavirus with the capsid protein and viral RNA. The estimated recovery rate was 80.2% by PCR and 90.0% by infection analysis, while the concentrating factor was 7.9-fold by PCR and 6.7-fold by infection analysis. In addition, the absence of non-specific binding to the antibody-integrated MNPs was confirmed using anti-dengue virus antibody-integrated MNBs as a negative control. Conclusion: These results suggest that this capture procedure is a useful tool for recovery and enrichment of infectious rotavirus. Moreover, when combined with a suitable virus assay this capture procedure can increase the sensitivity of rotavirus detection. Therefore, this capture method is a valuable tool for generating vaccines as well as for developing sensitive detection systems for viruses.

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“…Bare SPIONs have serious limitations such as instability against oxidation in air, dissolution in acids, and agglomeration [4]. Therefore, using protective and “antibiofouling” layers or conjugation of various functional ligands such as peptides, aptamers, antibodies or small molecules that possess strong affinity toward unique molecular signature overexpressed in pathogenic sites on the surface of SPIONs become an important issue to make them more compatible to nanomedicinal applications [5].…”

Section: Introductionmentioning

confidence: 99%

Receptor-Meditated Endocytosis by Hyaluronic Acid@Superparamagnetic Nanovetor for Targeting of CD44-Overexpressing Tumor Cells

Lin

Lee

et al. 2016

Nanomaterials

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The present report proposes a more rational hyaluronic acid (HA) conjugation protocol that can be used to modify the surface of the superparamagnetic iron oxide nanoparticles (SPIONs) by covalently binding the targeting molecules (HA) with glutamic acid as a molecular linker on peripheral surface of SPIONs. The synthesis of HA-Glutamic Acid (GA)@SPIONs was included oxidization of nanoparticle’s surface with H2O2 followed by activation of hydroxyl group and reacting glutamic acid as an intermediate molecule demonstrating transfection of lung cancer cells. Fourier transform infrared (FTIR) and zeta-potential studies confirmed the chemical bonding between amino acid linker and polysaccharides. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay showed that HA-SPIONs-treated cells remained 82.9% ± 2.7% alive at high particle dosage (200 µg/mL iron concentration), whereas GA-SPIONs and bare SPIONs (B-SPIONs) treated cells had only 59.3% ± 13.4% and 26.5% ± 3.1% survival rate at the same conditions, respectively. Confocal microscopy analysis showed increased cellular internalization of HA-SPIONs compared to non-interacting agarose coated SPIONs (AgA-SPIONs).

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Plasma-activated immobilization of biomolecules onto graphite-encapsulated magnetic nanoparticles

Cited by 51 publications

References 26 publications

Surface Character of Iron Oxide/Carbon Nanoparticles Synthesized by Submerged Arc Discharge Method in Ethanol/Urea Medium

Surface Character of Iron Oxide/Carbon Nanoparticles Synthesized by Submerged Arc Discharge Method in Ethanol/Urea Medium

<p>Efficient recovery and enrichment of infectious rotavirus using separation with antibody-integrated graphite-encapsulated magnetic nanobeads produced by argon/ammonia gas plasma technology</p>

Receptor-Meditated Endocytosis by Hyaluronic Acid@Superparamagnetic Nanovetor for Targeting of CD44-Overexpressing Tumor Cells

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