Plasma activated water as a pre-treatment strategy in the context of biofilm-infected chronic wounds

Vyas, Heema K.N.; Xia, Binbin; Alam, David; Gracie, Nicholas P.; Rothwell, Joanna G.; Rice, Scott A.; Carter, Dee; Cullen, Patrick J.; Mai-Prochnow, Anne

doi:10.1016/j.bioflm.2023.100154

Cited by 5 publications

(1 citation statement)

References 48 publications

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“…[ 27 ] Plasma‐activated water (PAW), enriched with a variety of reactive species, exhibits potent bacteria inactivation capabilities and emerges as a promising disinfectant and a treatment strategy in biofilm‐infected chronic wounds for the biomedical field. [ 28–30 ] PAW was also used for the inactivation of oral pathogenic bacteria, and Li et al proved that the PAW activated by air plasma jet for 20 min could inactivate more than 2.1‐log 10 planktonic S. mutans and 4.0‐log 10 planktonic P. gingivalis after 20‐s treatment. [ 31 ] Tasaki et al found that a 10‐s application of PAW prepared by DBD with the working gases of the mixture of helium and nitrogen for 180 s could reduce approximately 5.8‐log 10 planktonic S. mutans in an infected dentin model.…”

Section: Introductionmentioning

confidence: 99%

Efficient inactivation effect of plasma‐activated water on oral pathogens Streptococcus mutans and Porphyromonas gingivalis

Jia,

Song,

Dong

et al. 2024

Plasma Processes & Polymers

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Oral diseases stemming from oral pathogenic bacteria pose a significant global health concern, and current methods for managing these pathogens have limitations. Plasma‐activated water (PAW), containing various reactive species, emerges as a promising disinfectant with impressive inactivation capabilities. In this study, PAW prepared by mixed‐mode plasma‐activated gases was applied to inactivate oral pathogenic bacteria, including Streptococcus mutans and Porphyromonas gingivalis. The PAW could reduce more than 6.1‐log10 planktonic bacteria and 4.1‐log10 bacteria within biofilm, respectively, and PAW treatment of planktonic bacteria effectively inhibited biofilm formation. Compared to chlorhexidine, PAW exhibited superior inactivation effects in both planktonic bacteria and biofilm. This study presented a potent strategy for bacteria eradication to reduce the incidence of oral diseases.

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Section: Introductionmentioning

confidence: 99%

Efficient inactivation effect of plasma‐activated water on oral pathogens Streptococcus mutans and Porphyromonas gingivalis

Jia,

Song,

Dong

et al. 2024

Plasma Processes & Polymers

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Effects of cold atmospheric plasma‐treated medium on HaCaT and HUVEC cells in vitro

Chen,

Xie

et al. 2024

Plasma Processes & Polymers

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Cold atmospheric plasma (CAP) is an emerging technology that can generate various reactive oxygen and nitrogen species (RONS) at room temperature and shows promising applications for skin wound healing. The effects of plasma‐treated medium (PTM) promoting cell proliferation have been verified, but the biological mechanisms involved are not well known. This study aims to assess the proliferation effect and mechanism induced by PTM on human keratinocyte cells (HaCaT) and human umbilical vein endothelial cells (HUVEC) in vitro. The results showed that the concentrations of H2O2 and NO2− inside PTM increased in a time‐dependent manner as the atmospheric pressure plasma jet (APPJ) treatment time was prolonged. The biological outcomes were determined by cell counting kit, wound healing assay, flow cytometry, enzyme‐linked immunosorbent assay (ELISA), and western blot analysis. The cell viability and motility assays indicated that appropriate plasma conditions of short treatment time (15s‐, 30s‐ and 45s‐PTM) could promote cell proliferation, while long treatment time would inhibit cell proliferation (60s‐PTM). With an appropriate time of PTM treatment, the secretion of vascular endothelial growth factor‐α (VEGF‐α) and transforming growth factor alpha (TGF‐α) was promoted and the extracellular signal‐regulated kinase/serine‐threonine protein kinase pathways were activated, which induced HaCaT and HUVEC cell proliferation eventually. These behaviors of cells were mainly related to the enhancement of intracellular reactive oxygen species levels. These findings established a theoretical foundation for potential clinical applications of PTM in wound healing.

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Chitosan Hydrogels with Antibacterial and Antifungal Properties: Enhanced Properties by Incorporating of Plasma Activated Water

Cuéllar-Gaona,

González-López,

Martínez-Ruiz

et al. 2024

Plasma Chem Plasma Process

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Plasma activated water as a pre-treatment strategy in the context of biofilm-infected chronic wounds

Cited by 5 publications

References 48 publications

Efficient inactivation effect of plasma‐activated water on oral pathogens Streptococcus mutans and Porphyromonas gingivalis

Efficient inactivation effect of plasma‐activated water on oral pathogens Streptococcus mutans and Porphyromonas gingivalis

Effects of cold atmospheric plasma‐treated medium on HaCaT and HUVEC cells in vitro

Chitosan Hydrogels with Antibacterial and Antifungal Properties: Enhanced Properties by Incorporating of Plasma Activated Water

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