Plasma active and inactive renin in the rabbit: effect of dietary sodium depletion and repletion.

Grace, S A; Munday, K. A.; Noble, A. R.; Richards, Hugh K.

doi:10.1113/jphysiol.1979.sp012861

Cited by 16 publications

(17 citation statements)

References 16 publications

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“…However, experimental manipulations likely to result in changes in Na handling by the kidney have opposing effects on the secretion of active and of inactive renin. An increase in active renin accompanied by inhibition of inactive renin secretion has been found following a reduction in the [Na+] bathing kidney cortex slices , in the plasma of rabbits fed a low Na diet (Grace, Munday, Noble & Richards, 1979) and in anaesthetized rabbits (Richards et at. 1981) and anaesthetized sheep (Lush, Munday & Nobel, 1983) during furosemide diuresis.…”

Section: Discussionmentioning

confidence: 99%

Secretion control for active and inactive renin: effect of ouabain on release from rabbit kidney cortex slices.

Ginesi¹,

Noble²

1986

The Journal of Physiology

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SUMMARY1. Release of active and inactive renin by rabbit kidney cortex slices was investigated. Inactive renin was estimated as the increase in renin activity after acidification (pH 2 8) of slice supernatant solutions.2. For kidney slices incubated in complete Krebs bicarbonate buffer, the Na-K-ATPase inhibitor ouabain (100 lm) reduced the secretion of both active (-19-2 %) and inactive (-78-9 %) forms of renin.3. In low Na buffers ([Na+] = 23 mM) active renin release was increased and inactive renin was suppressed. Both of these changes were abolished by addition of ouabain (100 /tM). The reduction in inactive renin secretion produced by ouabain in complete Krebs buffer did not occur in low [Na+] buffers.4. In zero Ca2+ buffers containing EGTA (5 mM), secretion of both active and inactive renin was increased but these changes were abolished by addition of ouabain (100 /tm).5. Incubating kidney slices in low Na+, zero Ca2+ media revealed differences between the secretion control mechanisms for the two forms of renin. The separate stimulatory effects of low Na+ and low Ca2+ were not additive for the release of active renin and inclusion of ouabain resulted in similar secretion rates to those under control conditions. For inactive renin secretion, in the absence of Ca2+ release mechanisms still respond to reduction in Na+ with decreased secretion. Conversely, in low Na+ buffers, removal of Ca2+ still promotes inactive renin secretion. These changes were abolished by the addition of ouabain (100 SUM).6. Slices did not change in weight during incubation in media which did not contain ouabain. Addition of this inhibitor to control buffers and low Na buffers did result in an increase in weight. This correlated with the presence of Ca2+ in the buffer and did not appear to be related to [Na+].7. These studies again show that the mechanisms regulating the secretion of active and inactive renin are not identical and support the hypothesis that Na+ have differing roles to play in the regulation of these two forms of renin.

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Section: Discussionmentioning

confidence: 99%

Secretion control for active and inactive renin: effect of ouabain on release from rabbit kidney cortex slices.

Ginesi¹,

Noble²

1986

The Journal of Physiology

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“…Thus, although an inverse relationship between plasma active renin concentration and plasma inactive renin concentration has sometimes been observed during stimulation of the renin-angiotensin system (Derkx et al, 1976;Grace et al, 1979), there is no evidence to suggest that the inactive renin circulating in plasma is a major precursor of circulating active renin.…”

Section: Physiological Role Of Inactive Reninmentioning

confidence: 95%

Inactive Renin: An Attempt at a Perspective

Leckie

1981

Clinical Science

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“…However, dietary sodium depletion, a situation well known to increase circulating active renin levels, was initially associated with a decrease in inactive renin. Subsequent sodium repletion resulted in an increased proportion of plasma renin in the inactive form (Grace, Munday, Noble & Richards, 1979). This suggests that some aspect of the renal handling of sodium may determine the rate of activation or secretion of inactive renin from the kidney.…”

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confidence: 96%

“…This proportion is not altered by acute haemorrhage (Richards, Grace, Noble & Munday, 1979). However, dietary sodium depletion, a situation well known to increase circulating active renin levels, was initially associated with a decrease in inactive renin.…”

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Communications

1979

The Journal of Physiology

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The soleus muscle of most animals exhibits a slow contraction and contains principally fibres which are low in myofibrillar ATPase but rich in oxidative enzymes (type I). The motor unit compositions of cat (McPhedran, Wuerker & Henneman, 1965) and rat soleus (Close, 1967) reflect these properties. In contrast the mouse soleus contains a high proportion (about 60 %) of type II fibres and its contraction speed is intermediate between that of typically fast muscles, such as rat EDL, and pure slow muscles, such as cat or guinea-pig soleus. We have, therefore, examined the motor unit profile of soleus muscles from normal mice of the C57 B1/6J strain. Mice were anaesthetized with sodium pentobarbitone, 60 mg/kg i.r. A fine silicone rubber tube was sealed into the peritoneal cavity with cyanoacrylate adhesive, and used to maintain anaesthesia. Units were isolated by splitting ventral root filaments in the conventional manner. Immediately after the laminectomy, mice weighing about 25 g were given an infusion of 0 5-1 0 ml. of whole citrated blood. This was found to prolong survival time and to improve the condition of the preparation. In forty-seven motor units tetanic tensions, expressed as a percentage of whole muscle tension, showed little variation, ranging from 1-20 to 9-6%. The mean value was 4-60 % suggesting that soleus contains about twenty-two motor units. Time to peak tension showed a much greater variation, ranging from 7 to 37 msec in ten solei with whole muscle times to peak between 13 1 and 21-6 msec. No clear division into two groups of fast and slow units, as observed in rat soleus by Close (1967), was apparent in mouse soleus. As also observed in the cat (Bagust, 1974) in individual muscles a linear relationship was found between axonal conduction velocity and time to peak tension. Mouse soleus contains some motor units with times to peak as short as those in rat fast muscle and others almost as slow as those in rat soleus (Close, 1967).

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Plasma active and inactive renin in the rabbit: effect of dietary sodium depletion and repletion.

Cited by 16 publications

References 16 publications

Secretion control for active and inactive renin: effect of ouabain on release from rabbit kidney cortex slices.

Secretion control for active and inactive renin: effect of ouabain on release from rabbit kidney cortex slices.

Inactive Renin: An Attempt at a Perspective

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