Plasma Adsorption and Bridge Anticoagulant

Bridge Anticoagulant Neutralizing Agent: Properties and Isolation

“…On the other hand, evidence has been provided showing that the presence of a specific blood-clotting inhibitor, Bridge anti coagulant, reduces the in vivo effect of factor VIII (22,23). Follow ing the latter work it was speculated that should Bridge anticoagu lant be removed or its activity be neutralized, it would be possible to attain a marked improvement in the formation of plasma throm boplastin in haemophilic blood by using relatively small amounts of factor VIII.Recent investigations (17,19) have demonstrated that an agent which seems to stem from thrombin-fibrinogen interaction, renders inert Bridge anticoagulant, hence the absence of the latter in serum.Further experiments were carried out in an endeavour to iso late this neutralizing agent from fibrinogen/thrombin mixtures sub …”

“…Methods Reagents were prepared as described by N our-E ldin (19,21). Although the pre sent work was carried out using platelet suspension, practically the same results are ob tained on utilizing inosithin phospholipid as platelet-substitute (giving a substrate clotting-time of 9-10 seconds with normal reagents).…”

“…Studies on defibrination (19) have denoted that BANA can be furnished by low concentrations of exogenous thrombin. In the light of this information, defective thrombin generation cannot be accepted as a reason for the absence of BANA in haemophilic and Christmas disease sera.…”