Plasma alkaline phosphatase isoenzymes in hepatobiliary disease

Afonja, A O; Baron, Dror

doi:10.1136/jcp.27.11.916

Cited by 9 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kaplan and Rogers (1969) reported that, in their series, four patients with bone disease alone had a liver type main band, and in two of these there was no reason to suspect liver disease, while Sussman et al (1968) found that the AP isoenzymes of liver, bone, kidney, and intestine all moved with indistinguishable mobilities. More recently, Afonja and Baron (1974) found that normal adult serum always contained a slow 'liver' band and sometimes a bone band. Many of the discrepancies are accounted for by the fact that interpretation of the results obtained by electrophoretic separation of the alkaline phosphatase isoenzymes in serum has until now been largely subjective.…”

mentioning

confidence: 99%

Polyacrylamide gel disc electrophoresis of alkaline phosphatase isoenzymes in bone and liver disease.

Warnes¹,

Hine²,

Kay³

1976

J Clin Pathol

View full text Add to dashboard Cite

Royal Infirmary, Manchester M13 9WL SYNOPSiS Acrylamide gel disc electrophoresis provides a reliable and reasonably rapid method of differentiating the raised serum alkaline phosphatase (AP) of bone origin from that of liver origin. The technique has been placed for the first time on a semiquantitative basis. Measurement of both band width and band position effectively distinguishes the bone from the liver isoenzyme, but band width provides superior discrimination.An origin band was seen in none of the normal subjects and in only 7 % of patients with bone disease but was present in 78 % of patients with liver disease, a highly significant increase. Fifty percent of normal individuals had a small-intestinal band in serum taken two hours after a meal, as did 35 % of patients with liver disease, but the incidence of intestinal bands in bone disease was only 11 %, significantly less than in the other two groups. The genetic control of small-intestinal AP in serum has been confirmed, but it has been demonstrated that the decrease of intestinal AP in bone disorders is not genetically determined.

show abstract

mentioning

confidence: 99%

Polyacrylamide gel disc electrophoresis of alkaline phosphatase isoenzymes in bone and liver disease.

Warnes¹,

Hine²,

Kay³

1976

J Clin Pathol

View full text Add to dashboard Cite

show abstract

“…Alkaline phosphatases comprise a group of enzymes that catalyze the hydrolysis of a number of phosphate esters, in an alkaline environment, generating an organic radical and inorganic phosphate. Alkaline phosphatase is a membrane-bound metalloenzyme comprising a group of isoenzymes encoded by at least four different gene loci (Afonja and Baron, 1974). In healthy adults, this enzyme is mainly derived from the liver, bones and in a lesser amount from the intestine, placenta, kidneys, and leukocytes (Friedman, 1996).…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective Effects of Various Solvent Extracted Samples of Paeonia Emodi (wall) in Mice with Paracetamol-Induced Hepatotoxicity

Zeb¹,

Shafiq²,

Khan³

et al. 2018

JBLS

View full text Add to dashboard Cite

The goal of present study is to evaluate and recognize the most effective hepatoprotective solvent fractions of methanolic extract of Paeonia emodi by fractionating and assessing its fractions for hepatoprotective effect in paracetamol-induced liver damage in mice. In this study, some liver-related serum parameters such as AST, ALP, and ALT were studied. Maximum reduction was caused by aqueous extract (61.08 %) followed by a butanol extract (60.26 %), chloroform extract (48.76 %), crude extract (45.48 %) and drug (37.93 %), in case of ALT. During the study of AST, chloroform extract caused a maximum reduction (65.94 %), followed by a butanol extract (60.43 %), aqueous extract (57.13 %), crude extract (57.13 %) and drug (43.94 %). During the study of ALP aqueous fraction caused a maximum reduction (47.22 %), followed by a butanol fraction (44.44 %), chloroform (41.51 %), methanol extract (34.72 %) and drug (20.83 %). In all the treatment groups the serum levels of ALT, AST and ALP were significantly lower as compared to those of untreated controlled group (P<0.05). The present research showed that Paeonia emodi has the potential to reduce paracetamol-induced hepatotoxicity in mice and restore the normal liver function.

show abstract

“…Previous investigators, using different separating media, have identified the presence of a fast liver band in patients with liver disease (Fritsche and Adams-Park, 1974;Afonja and Baron, 1974), and this band may represent the slow moving isoenzyme noted in polyacrylamide gel electrophoresis (Fritsche and Adams-Park, 1974;Price and Sammons, 1974). De Broe et al, l975 found that the high molecular weight alkaline phosphatase with rapid migration on cellulose acetate electrophoresis corresponded to a membrane-vesicle fraction seen with electron microscopy in patients with cholestasis.…”

mentioning

confidence: 96%