Plasma and Blood Volume in an Æmia and the Effect of Transfusion

The sources of bile pigment in the rat: studies of the "early labeled" fraction.

“…This resulted in an estimated blood volume of 7.4%, compared to 6.0% in normal rats of similar weight. d) In rats with anemia, we assumed that the blood volume fell 5% for each 10% reduction in hemoglobin concentration (40,41).…”

Hemolysis of “stress” reticulocytes: a source of erythropoietic bilirubin formation

“…Production of labeled hemoglobin heme was expressed as per cent incorporation of glycine-"C, calculated from the specific activity of hemoglobin hemin and the hemin equivalent of the rat's blood volume (25). It was assumed that in anemic rats blood volume decreased 5%o for each 10%o fall in hemoglobin concentration (26,27).…”

“…(b) To estimate the total magnitude of bilirubin-"4C production and to correct for variations in the amount of blood transfused in each experiment, the observed rates of labeled pigment excretion were adjusted to correspond to transfusions of an entire donor red cell mass. The average donor blood volume was calculated from the body weights of the donors used in each experiment (25), with correction for anemia (26,27) as appropriate. The observed rates of bilirubin-"C production in the recipients of this donor blood were then multiplied by the ratio between total donor hemoglobin and the amount of hemoglobin actually transfused.…”