Plasma and Red Cell Iron Turnover in Normal Subjects and in Patients Having Various Hematopoietic Disorders 1

Huff, Rex L.; Hennessy, Thomas; Austin, Rachel; Garcia, Joseph F.; Roberts, Brittney; Lawrence, John H.

doi:10.1172/jci102335

Cited by 325 publications

(84 citation statements)

References 19 publications

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“…Salt Lake City) (Received for publication, October 3, 1955) In the preceding publication (1) the results of studies on the kinetics of iron metabolism (2)(3)(4)(5) in normal growing swine were reported. The purpose of this paper is to present the results of similar studies in swine with three different types of experimentally induced anemias.…”

Section: (From the Department Of Medicine College Of Medicine Univementioning

confidence: 99%

The Kinetics of Iron Metabolism in Swine With Various Experimentally Induced Anemias

Bush

Jensen

Ashenbrucker

et al. 1956

The Journal of Experimental Medicine

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In the preceding publication (1) the results of studies on the kinetics of iron metabolism (2-5) in normal growing swine were reported. The purpose of this paper is to present the results of similar studies in swine with three different types of experimentally induced anemias. As an example of a severe hemolytic process with no detectable impairment in the capacity of the bone marrow to produce erythrocytes, anemia induced by the administration of phenylhydrazine (6) has been studied. The kinetics of iron metabolism have been studied also in swine with anemia due to a deficiency of pyridoxine (6--8) and in swine with anemia due to a deficiency of pteroylglutamic acid (9-11). The last two anemias are quite different morphologically. That anemia associated with pyridoxine deficiency is microcytic and hypochromic, whereas that occurring in pteroylglutamic acid deficiency is macrocytic. In both, the plasma iron level is greatly elevated, there is a reduction in the amount of free protoporphyrin in the erythrocytes, and there is erythroid hyperplasia in the bone marrow. However, the tissues of the pyridoxinedeficient animals are heavily laden with iron, whereas hemosiderin deposits are not prominent in the tissues of swine deficient in pteroylglutamic acid.These studies were carried out in the hope of gaining an understanding of the pathogenesis of the anemia in these conditions and also with the expectation that these anemias of known and singular etiology might serve as patterns of reference for anemias which develop in human subjects. In a later publication (12) iron kinetics will be described in copper-deficient swine. The anemia accompanying that condition is microcytic and hypochromic and the level of iron in the plasma and tissues is low.

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Section: (From the Department Of Medicine College Of Medicine Univementioning

confidence: 99%

The Kinetics of Iron Metabolism in Swine With Various Experimentally Induced Anemias

Bush

Jensen

Ashenbrucker

et al. 1956

The Journal of Experimental Medicine

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The availability of radioactive iron (Fe 6~) of high specific activity and the development of the scintillation counter enabled Huff et al (1) to devise a technique for determining the turnover rates of iron through plasma and red cells.This method involves the intravenous injection of a tracer dose of Fe s9 containing a quantity of carrier iron sufficiently small that the plasma iron concentration will not be elevated measurably. The Fe s9 radioactivity disappears from the plasma according to the equation of a first order reaction and the time at which half of the injected dose has disappeared from the plasma (T~) is determined by plotting the counts of serial plasma samples on semilogarithmic paper.

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confidence: 99%

“…The availability of radioactive iron (Fe 6~) of high specific activity and the development of the scintillation counter enabled Huff et al (1) to devise a technique for determining the turnover rates of iron through plasma and red cells.…”

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The Kinetics of Iron Metabolism in Normal Growing Swine

Jensen

Bush

Ashenbrucker

et al. 1956

The Journal of Experimental Medicine

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The availability of radioactive iron (Fe 6~) of high specific activity and the development of the scintillation counter enabled Huff et al. (1) to devise a technique for determining the turnover rates of iron through plasma and red cells.This method involves the intravenous injection of a tracer dose of Fe s9 containing a quantity of carrier iron sufficiently small that the plasma iron concentration will not be elevated measurably. The Fe s9 radioactivity disappears from the plasma according to the equation of a first order reaction and the time at which half of the injected dose has disappeared from the plasma (T~) is determined by plotting the counts of serial plasma samples on semilogarithmic paper. The plasma iron turnover rate (PITR) in milligrams per day may be calculated from the formula: 0.693 X PV X PI X 24 --PITR r 1/2 in which PV is the plasma volume in milliliters, PI is the plasma iron concentration in milligrams per milliliter, 24 is the number of hours per day.On subsequent days the red cells are assayed for Fe 59 activity until a constant fraction of injected radioactivity is present in the erythrocytes. The red cell iron turnover rate (RBC ITR) in milligrams per day is calculated from the formula:in which PITR is the plasma iron turnover rate in milligrams per day, FeS, ' is the maximum fraction of injected Fe s9 incorporated into the erythrocytes.The total red cell iron (RBC I) in rag. may then be determined from the formula:TBV 100 X Hb X 3.4 = KBCI

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The Effects of Total Body Irradiation on Some Aspects of Human Iron Metabolism

Levin¹,

Andrews²,

Berlin³

1961

J. Clin. Invest.

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The utilization of radioactive iron to evaluate erythropoietic function in normal and disease states in man is well established (1)(2)(3)(4)(5)(6)(7)(8). These studies have demonstrated that the half-time (Ti) of plasma radioiron disappearance and the red cell uptake of radioactive iron are valid parameters of bone marrow function. Many investigators have demonstrated dose-dependent changes in these functions following total body irradiation of the rat (9-14). Similar changes have been noted following total body irradiaton of the burro and sheep (15), and monkey (16).Loeffler, Collins and Hyman (17) studied four patients who received from 50 to 150 r and did not note significant changes in the T4 of the plasma radioiron (Fe59) disappearance at 3 hours or 7 days. In three patients receiving from 150 to 200 r, Collins and Loeffler (18) noted an increase in the Tj at varying times after irradiation.In nine patients who had received 200 r to the anterior surface of the body. Loeffler (19) noted an increase in the Tj which was maximal 48 hours following irradiation. Suit, Lajtha, Oliver and Ellis (20) were unable to determine changes in the iron uptake of human bone marrow irradiated in vivo and then studied in a culture medium with Fe59 administered immediately or 24 hours after irradiation. However, they were able to demonstrate an increase in the To and some decrease in the red cell uptake of iron following 100 r total body irradiation, but only when the Fe59 was administered to their patients 48 to 72 hours after irradiation (21). No changes were noted following 25 to 50 r. Sinclair (22) studied 12 patients who received 200 r, and reported a significant increase in the Tj in all and a depression of red cell uptake of iron in two-thirds of these patients. One cannot construct a dose-response curve from these studies because of the variation in technique and in the source of radiation utilized.

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Plasma and Red Cell Iron Turnover in Normal Subjects and in Patients Having Various Hematopoietic Disorders 1

Cited by 325 publications

References 19 publications

The Kinetics of Iron Metabolism in Swine With Various Experimentally Induced Anemias

The Kinetics of Iron Metabolism in Swine With Various Experimentally Induced Anemias

The Kinetics of Iron Metabolism in Normal Growing Swine

The Effects of Total Body Irradiation on Some Aspects of Human Iron Metabolism

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