Plasma arginine vasopressin response to oral, gastric, and intravenous water load in patients with cirrhosis

Castellano, Giancarlo; Solis-Herruzo, JoséA.; González, A.; Morillas, Juan Diego; Moreno, Daniel; Muñoz, Teresa; Larrodera, L

doi:10.1016/0016-5085(94)90702-1

Cited by 4 publications

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Protoscoleces were pre-treated for 2 or 5 days with 0.8 μM or 1.6 μM PIK-75, and HO-1, NQO-1, GSH-Px and TPx expression was measured using an ELISA kit to examine whether PIK-75 modulates HO-1, NQO-1, GSH-Px and TPx expression in cultured protoscoleces (Figs. 4,5,6,7). All protoscoleces treated with PIK-75 showed a time-and dose-dependent decrease in HO-1, NQO-1, GSH-Px and TPx expression (P<0.05).…”

Section: Effects Of Pik-75 On the Expression Of Antioxidant Enzymes I...mentioning

confidence: 93%

“…In recent years, various local chemotherapy drugs have emerged either clinically or at the experimental stage (6). However, adverse side effects have been observed with both scolicidal agents, which may cause bile duct function abnormalities (7), and H 2 O 2 , which has not gained wide application owing to potential complications and low e cacy (8). The use of high concentrations of hypertonic saline solution has been found to be extremely effective for short periods against scolices of hydatid cysts.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro parasiticidal effects of PIK-75 inhibition of Nrf2 against Echinococcus granulosus protoscoleces

Tang

Liu

Wang

et al. 2023

Preprint

View full text Add to dashboard Cite

Introduction The aims of this study were to investigate the role of the nuclear factor E2-related factor 2 (Nrf2) signaling pathway in Echinococcus granulosus protoscoleces, and to examine the effects of PIK-75 inhibition on Nrf2 activity. Methods Nrf2 protein expression and localization in protoscoleces were examined via confocal immunofluorescence microscopy. Reactive oxygen species (ROS detection kit) was used to detect ROS level in protoscoleces. The effects of PIK-75 on activity of heme oxygenase1(HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1), glutathione peroxidase (GSH-Px) and thioredoxin peroxidase (TPx) were characterized using ELISA. Evidence of parasite damage and death was observed by scanning electron microscopy(SEM). Western bolting was used to detect the expression level of Nrf2 protein. In addition, caspase-3 activity was detected using an assay kit. Results The study found that Nrf2 is primarily localized in the protoscoleces cytoplasm, and PIK-75 treatment could increased ROS level(P<0.05) in the early time, and reduced NQO-1, HO-1, GSH-Px and TPx (P<0.05) activity in protoscoleces. SEM showed that PIK-75-treated protoscoleces presented damage in the protoscoleces region. Western-blot showed that the Nrf2 protein expression had decreased significantly. Caspase-3 activity clearly increased in protoscoleces treated for 24 and 48 h with PIK-75 compared with that in controls (P<0.05). Conclusion The present investigation demonstrated that PIK-75 had an inhibitory effect on the Nrf2 signaling pathway. We also provide evidence that PIK-75 may serve as a potential therapeutic agent for the treatment of protoscoleces. The use of PIK-75 as a treatment for protoscoleces, however, requires further research.

show abstract

Section: Effects Of Pik-75 On the Expression Of Antioxidant Enzymes I...mentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%