Plasma-based proteomics reveals immune response, complement and coagulation cascades pathway shifts in heat-stressed lactating dairy cows

Li, Min; Cheng, Jianbo; Zhao, Shengguo; Tian, He; Zhang, Yangdong; Li, Songli; Hu, Yang; Zheng, Nan; Wang, Jiaqi

doi:10.1016/j.jprot.2016.06.008

Cited by 60 publications

(30 citation statements)

References 45 publications

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“…To our knowledge this is the first such study of complement proteins in social stress. Previously, a single study of heat stressed cows has observed complement activation [62]. Our psychosocial stress study shows interesting overlaps with the results of the PE study in demonstrating upregulated CFH, CFI (alternative pathway) and C1 complex subcomponent C1R (classical pathway).…”

Section: Discussionsupporting

confidence: 75%

Complement pathway changes at age 12 are associated with psychotic experiences at age 18 in a longitudinal population-based study: evidence for a role of stress

et al. 2019

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The complement cascade is a major component of the immune defence against infection, and there is increasing evidence for a role of dysregulated complement in major psychiatric disorders. We undertook a directed proteomic analysis of the complement signalling pathway (n = 29 proteins) using data-independent acquisition. Participants were recruited from the UK avon longitudinal study of parents and children (ALSPAC) cohort who participated in psychiatric assessment interviews at ages 12 and 18. Protein expression levels at age 12 among individuals who reported psychotic experiences (PEs) at age 18 (n = 64) were compared with age-matched controls (n = 67). Six out of the 29 targeted complement proteins or protein subcomponents were significantly upregulated following correction for multiple comparisons (VTN↑, C1RL↑, C8B↑, C8A↑, CFH↑, and C5↑). We then undertook an unbiased plasma proteomic analysis of mice exposed to chronic social stress and observed dysregulation of 11 complement proteins, including three that were altered in the same direction in individuals with PE (C1R↑, CFH↑, and C5↑). Our findings indicate that dysregulation of the complement protein pathway in blood is associated with incidence of psychotic experiences and that these changes may reflect exposure to stress.

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Section: Discussionsupporting

confidence: 75%

Complement pathway changes at age 12 are associated with psychotic experiences at age 18 in a longitudinal population-based study: evidence for a role of stress

et al. 2019

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“…In contrast to previous data in the literature (Min et al, 2016;Tian et al, 2015), the marked hypolactemia following S/C injection of either saline or IVM represents a major surprise in this study and explanation of this outcome needs more investigation.…”

Section: Figurecontrasting

confidence: 99%

Acute behavioural and physiological responses of sheep to S/C ivermectin injection

Mahmoud

Khalil

El-Sayed³

2019

Acta fytotechn zootechn

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Twenty multiparous, non-pregnant and non-lactating adult ewes and five native Egyptian breed rams (Ovis aries) weighing (35-45 kg) and aged 3-5 years, were used in this experiment. The sheep were randomly assigned to five groups [each had 4 ewes and one ram]; namely, a control group (C, without injection), two saline groups (SN and SE) (injected with 0.9% NaCl) and two IVM groups (IN and IE) [IVM at a dose of 0.2 mg kg-1 BW, 1% solution (Ivomec® injection Merk & Co. Inc., Rahway, NJ, USA)]. The injection was at the left neck region or behind the left elbow. The animals were penned individually for all ethological observations and collection of all data and samples.

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“…A range of complement proteins have recently been identified by our group to be deiminated in a range of taxa throughout the phylogenetic tree including teleost and cartilaginous fish, camelids, cetaceans, pinnipeds, camelids and peglagic seabirds [16,17,[91][92][93][94][95][96]98,99]. The bovine complement system [126] has been widely studied, including in immune defences against bovine anaplasmosis [127], in relation to heat-stress in dairy cows [128], susceptibility to bacterial infection relating to copper-deficiency [129]. Roles for modulation of complement regulation in relation to poxviral and pestiviral infections have been identified [130][131][132], including immune evasion by bovine herpesvirus 1 (BHV-1) [133], while and roles for antibody-dependent complement-mediated killing of mycoplasma have recently been revealed [134].…”

Section: Discussionmentioning

confidence: 99%

Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination

Criscitiello

Kraev

Lange

2020

IJMS

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The bovine immune system is known for its unusual traits relating to immunoglobulin and antiviral responses. Peptidylarginine deiminases (PADs) are phylogenetically conserved enzymes that cause post-translational deimination, contributing to protein moonlighting in health and disease. PADs also regulate extracellular vesicle (EV) release, forming a critical part of cellular communication. As PAD-mediated mechanisms in bovine immunology and physiology remain to be investigated, this study profiled deimination signatures in serum and serum-EVs in Bos taurus. Bos EVs were poly-dispersed in a 70-500 nm size range and showed differences in deiminated protein cargo, compared with whole sera. Key immune, metabolic and gene regulatory proteins were identified to be post-translationally deiminated with some overlapping hits in sera and EVs (e.g., immunoglobulins), while some were unique to either serum or serum-EVs (e.g., histones). Protein-protein interaction network analysis of deiminated proteins revealed KEGG pathways common for serum and serum-EVs, including complement and coagulation cascades, viral infection (enveloped viruses), viral myocarditis, bacterial and parasitic infections, autoimmune disease, immunodeficiency intestinal IgA production, B-cell receptor signalling, natural killer cell mediated cytotoxicity, platelet activation and hematopoiesis, alongside metabolic pathways including ferroptosis, vitamin digestion and absorption, cholesterol metabolism and mineral absorption. KEGG pathways specific to EVs related to HIF-1 signalling, oestrogen signalling and biosynthesis of amino acids. KEGG pathways specific for serum only, related to Epstein-Barr virus infection, transcription mis-regulation in cancer, bladder cancer, Rap1 signalling pathway, calcium signalling pathway and ECM-receptor interaction. This indicates differences in physiological and pathological pathways for deiminated proteins in serum-EVs, compared with serum. Our findings may shed light on pathways underlying a number of pathological and anti-pathogenic (viral, bacterial, parasitic) pathways, with putative translatable value to human pathologies, zoonotic diseases and development of therapies for infections, including anti-viral therapies.

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Plasma-based proteomics reveals immune response, complement and coagulation cascades pathway shifts in heat-stressed lactating dairy cows

Cited by 60 publications

References 45 publications

Complement pathway changes at age 12 are associated with psychotic experiences at age 18 in a longitudinal population-based study: evidence for a role of stress

Complement pathway changes at age 12 are associated with psychotic experiences at age 18 in a longitudinal population-based study: evidence for a role of stress

Acute behavioural and physiological responses of sheep to S/C ivermectin injection

Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination

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