Plasma Cells, the Next Generation: Beyond Antibody Secretion

Pioli, Peter D.

doi:10.3389/fimmu.2019.02768

Cited by 60 publications

(40 citation statements)

References 78 publications

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“…36 ). In addition to B cells, production of proinflammatory cytokines can also be carried out by ASCs 43,44 , potentially explaining the correlation between the expansion of these cells and severe COVID-19 infection. The production of pathogenic cytokines by ASCs and/or EF B cells in COVID-19 remains to be experimentally addressed.…”

Section: Discussionmentioning

confidence: 99%

Extrafollicular B cell responses correlate with neutralizing antibodies and morbidity in COVID-19

et al. 2020

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A wide spectrum of clinical manifestations has become a hallmark of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) COVID-19 pandemic, although the immunological underpinnings of diverse disease outcomes remain to be defined. We performed detailed characterization of B cell responses through high-dimensional flow cytometry to reveal substantial heterogeneity in both effector and immature populations. More notably, critically ill patients displayed hallmarks of extrafollicular B cell activation and shared B cell repertoire features previously described in autoimmune settings. Extrafollicular activation correlated strongly with large antibody-secreting cell expansion and early production of high concentrations of SARS-CoV-2-specific neutralizing antibodies. Yet, these patients had severe disease with elevated inflammatory biomarkers, multiorgan failure and death. Overall, these findings strongly suggest a pathogenic role for immune activation in subsets of patients with COVID-19. Our study provides further evidence that targeted immunomodulatory therapy may be beneficial in specific patient subpopulations and can be informed by careful immune profiling.

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Section: Discussionmentioning

confidence: 99%

Extrafollicular B cell responses correlate with neutralizing antibodies and morbidity in COVID-19

et al. 2020

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“…The numerous epitope antigen activates a large number of lymphocytes (Lipman et al, 2005). Further, the lymphocytes proliferate and differentiate into plasma cells that synthesize antibodies (Pioli, 2019). In the current study, the produced antibodies were determined as the polyclonal antibody.…”

Section: Resultsmentioning

confidence: 95%

Production of Newcastle Disease Polyclonal Antibody as the Alternative of Immunohistochemistry Primary Antibody against Newcastle Disease in Poultry

Naf’an¹,

Kurniasih²,

Untari³

et al. 2021

WVJ

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Newcastle disease (ND) is the most pathogenic viral infection in poultry. Furthermore, the availability of laboratories that support the molecular diagnosis of ND is still limited in Indonesia. The present study aimed to produce ND polyclonal antibody as the alternative of immunohistochemistry primary antibody against ND in poultry. Two adult male New Zealand White rabbits weighed 2.5 kg were vaccinated seven days after the adaptation using intraperitoneal injection of the ND live vaccine at multilevel doses weekly. The serum was collected inactivated, and purified in the sixth week. A total number of 31 chicken samples were collected and their samples of brain, lung, spleen, and intestine were tested using immunohistochemistry and Reverse Transcription Polymerase Chain reaction (RT-PCR). The result showed that 19/31 (61%) were positive against immunohistochemistry and RT-PCR and a total of 12/31 (39%) were negative. Based on the obtained results, immunohistochemistry using ND polyclonal antibody had a similar accuracy with RT-PCR. It can be concluded that ND polyclonal antibody produced by vaccination in the rabbit could be used as the alternative immunohistochemistry primary antibody for diagnosing ND in poultry.

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“…Furthermore, evidence suggests that Bregs have the ability to differentiate into IL‐10‐producing plasmablasts and plasma cells in vitro and in vivo 22,23 . Although antibody‐producing plasmablasts/plasma cells are largely associated with pro‐inflammatory responses, 24 a subset of IL‐10 + regulatory plasmablasts have been shown to suppress immune responses while producing antibody 25,26 . These findings suggest that B cells at any stage of development can exhibit a regulatory phenotype.…”

Section: Overview Of Breg Induction Phenotype and Functionmentioning

confidence: 99%

Regulatory B cells in respiratory health and diseases

Menon

Hussell

Shuwa

2021

Immunological Reviews

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B cells are critical mediators of humoral immune responses in the airways through antibody production, antigen presentation, and cytokine secretion. In addition, a subset of B cells, known as regulatory B cells (Bregs), exhibit immunosuppressive functions via diverse regulatory mechanisms. Bregs modulate immune responses via the secretion of IL‐10, IL‐35, and tumor growth factor‐β (TGF‐β), and by direct cell contact. The balance between effector and regulatory B cell functions is critical in the maintenance of immune homeostasis. The importance of Bregs in airway immune responses is emphasized by the different respiratory disorders associated with abnormalities in Breg numbers and function. In this review, we summarize the role of immunosuppressive Bregs in airway inflammatory diseases and highlight the importance of this subset in the maintenance of respiratory health. We propose that improved understanding of signals in the lung microenvironment that drive Breg differentiation can provide novel therapeutic avenues for improved management of respiratory diseases.

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Plasma Cells, the Next Generation: Beyond Antibody Secretion

Cited by 60 publications

References 78 publications

Extrafollicular B cell responses correlate with neutralizing antibodies and morbidity in COVID-19

Extrafollicular B cell responses correlate with neutralizing antibodies and morbidity in COVID-19

Production of Newcastle Disease Polyclonal Antibody as the Alternative of Immunohistochemistry Primary Antibody against Newcastle Disease in Poultry

Regulatory B cells in respiratory health and diseases

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