Plasma cyclophosphamide assay by selective ion monitoring

Lartigue-Mattei, C.; Chabard, J. L.; Touzet, C.; Bargnoux, H.; Petit, Jean-Pierre; Berger, J.A.

doi:10.1016/0378-4347(84)80108-5

Cited by 13 publications

(3 citation statements)

References 7 publications

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“…The liquid extraction procedures were adapted from previous work reported by Lartigue-Mattei, 8 Mehta" and Jardine,6 the solid-phase extraction procedures were based on previous work from El-Yazigi," Burton" and Hardy.13 The solid-phase extraction cartridges can only be applied at acidic and neutral pH values. The results of these experiments are presented in Table 2.…”

Section: Recovery Of Cpa Metabolites From Spiked Plasma Samplesmentioning

confidence: 99%

The determination of cyclophosphamide and its metabolites in blood plasma as stable trifluoroacetyl derivatives by electron capture chemical ionization gas chromatography/mass spectrometry

Momerency¹,

Cauwenberghe²,

Slee³

et al. 1994

Biol. Mass Spectrom.

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A method is described for the determination of the antitumour drug cyclophosphamide and six stable metabolites in plasma of cancer patients, namely dechloroethyl-cyclophosphamide, 4-keto-cyclophosphamide, carboxy-phosphamide, alcophosphamide, nor-nitrogen mustard and the N-chloroethyl-1,3-oxazolidine-2-one, as methyl and/or trifluoroacetyl derivatives by single ion monitoring gas chromatography/mass spectrometry, mostly in the electron capture chemical ionization mode. The isolation of most metabolites was performed by solid-phase C-18 extraction in weakly acidic medium. The phosphoramide mustard isolated under these conditions decomposes readily to the nor-nitrogen mustard during derivatization. The original nor-nitrogen mustard and the chloroethyl-1,3-oxazolidine-2-one were isolated by liquid extraction with ethyl acetate in alkaline medium. Recoveries of 75-99% were measured using spiked blank plasma samples. Quantitation of metabolites in patient plasma samples was performed using two sets of calibration curves for the concentration ranges of 1-100 ng and 0.1-10 micrograms of metabolite per millilitre of original plasma.

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Section: Recovery Of Cpa Metabolites From Spiked Plasma Samplesmentioning

confidence: 99%

The determination of cyclophosphamide and its metabolites in blood plasma as stable trifluoroacetyl derivatives by electron capture chemical ionization gas chromatography/mass spectrometry

Momerency¹,

Cauwenberghe²,

Slee³

et al. 1994

Biol. Mass Spectrom.

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show abstract

“…spectrometry [13], gas chromatography-mass spectrometry [14,15], gas-liquid chromatography, both with and without derivatization , high performance liquid chromatography [21], and high performance liquid chromatography-field desorption mass spectrometry [22]. Since radiolabeling is clinically inconvenient and techniques using mass spectrometry hamper routine application in extensive pharmacological studies, gas chromatography with nitrogen phosphorus specific detection and high performance liquid chromatography seem attractive methods .…”

Section: Introductionmentioning

confidence: 99%

The autoderivatization of 4‐ketocyclophosphamide during capillary gas chromatography

Bruijn¹,

Leclercq²,

Tjaden³

1986

J. High Resol. Chromatogr.

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The selectivity of a capillary gas chromatographic assay for the anti‐neoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4‐ketocyclophosphamide (4‐keto CPA), has been studied. Mass spectrometry studies showed that a cyclization product of 4‐keto‐CPA can form using the same chromatographic conditions as those under which CPA was determined. However, the amount produced is relatively small (less than 10%) compared with the percentage produced from CPA. Furthermore, both cyclization products, formed by loss of HCI and intraalkylation of the parent compounds, can be separated well under suitable chromatographic conditions.

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“…11 Further, methods have been formulated for the evaluation of antineoplastic drug exposure among hospital personnel, as presented in Table 1. 4,11-21…”

mentioning

confidence: 99%

Efforts to Ensure Safety of Hospital Pharmacy Personnel Occupationally Exposed to Antineoplastic Drugs during a Preparation Task

et al. 2007

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Purpose Antineoplastic drugs are often detected in the plasma and urine of medical staff, despite the adoption of safety measures. This study involves ambulatory chemotherapy, which was prepared on a rotating schedule at the Department of Pharmacy. The first objective of this study was to evaluate occupational exposure by measuring epirubicin in the urine and plasma and sister chromatid exchanges (SCE) in the peripheral lymphocytes of the involved pharmacists. The second, to improve a previously reported method for ease of occupational exposure evaluation. The third, to determine environmental contamination. Methods Pharmacists were categorized into three groups based on three patterns, depending on the other tasks they performed. They were alternately responsible for the preparation in each group with the recommended protections. Samples were collected at the end of each work day. High-performance liquid chromatography (HPLC) analysis along with fluorescence detection was performed at a flow rate of 200 mcL/min using 0.1% formic acid, methanol, and acetonitrile (75:5:20). Epirubicin in the samples was extracted by solid-phase extraction. Chromosomes were prepared according to a standard protocol. Results A final concentration of 2 ng/mL was the lower quantification limit. Epirubicin was not detected in the urine and plasma, and the SCE levels were within the normal limits for all the subjects. However, epirubicin was detected in samples collected from the preparation gloves. Conclusions For personnel safety, it is important to periodically evaluate protective measures. Pharmacists on a rotating schedule who work less than 6 hours per week on preparatory tasks were not unduly exposed to antineoplastics.

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Plasma cyclophosphamide assay by selective ion monitoring

Cited by 13 publications

References 7 publications

The determination of cyclophosphamide and its metabolites in blood plasma as stable trifluoroacetyl derivatives by electron capture chemical ionization gas chromatography/mass spectrometry

The determination of cyclophosphamide and its metabolites in blood plasma as stable trifluoroacetyl derivatives by electron capture chemical ionization gas chromatography/mass spectrometry

The autoderivatization of 4‐ketocyclophosphamide during capillary gas chromatography

Efforts to Ensure Safety of Hospital Pharmacy Personnel Occupationally Exposed to Antineoplastic Drugs during a Preparation Task

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