Plasma cytokine levels and cytokine gene polymorphisms in Mexican patients during the influenza pandemic A(H1N1)pdm09

Martinez-Ocaña, Joel; Olivo-Dı́az, Angélica; Salazar-Dominguez, Tobías; Reyes-Gordillo, Jesus; Tapia-Aquino, Cynthia; Martínez‐Hernández, Fernando; Manjarrez, María Eugenia; Antonio-Martinez, Marco; Contreras-Molina, Araceli; Figueroa-Moreno, Rafael; Valdez-Vázquez, Rafael; Kawa-Karasik, Simón; Rodríguez‐Zulueta, Patricia; Flisser, Ana; Maravilla, Pablo; Romero‐Valdovinos, Mirza

doi:10.1016/j.jcv.2013.05.013

Cited by 36 publications

(46 citation statements)

References 29 publications

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“…However in the human cohort the fold increases in cytokine levels was more modest and the temporal cytokine flux was more complex. Although the significant differences in types and clinical characteristics of cohorts reported in literature prevented direct comparison of our results, elevation of inflammatory cytokines and chemokines early during presentation, and the levels we have observed, are consistent with reports in literature [22], [23], [24]. Additionally, some of these differences could also be attributed to different immune response to different viral strains.…”

Section: Discussionsupporting

confidence: 91%

Molecular Analysis of Serum and Bronchoalveolar Lavage in a Mouse Model of Influenza Reveals Markers of Disease Severity That Can Be Clinically Useful in Humans

et al. 2014

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BackgroundManagement of influenza, a major contributor to the worldwide disease burden, is complicated by lack of reliable methods for early identification of susceptible individuals. Identification of molecular markers that can augment existing diagnostic tools for prediction of severity can be expected to greatly improve disease management capabilities.Methodology/Principal FindingsWe have analyzed cytokines, proteome flux and protein adducts in bronchoalveolar lavage (BAL) and sera from mice infected with influenza A virus (PR8 strain) using a previously established non-lethal model of influenza infection. Through detailed cytokine and protein adduct measurements of murine BAL, we first established the temporal profile of innate and adaptive responses as well as macrophage and neutrophil activities in response to influenza infection. A similar analysis was also performed with sera from a longitudinal cohort of influenza patients. We then used an iTRAQ-based, comparative serum proteome analysis to catalog the proteome flux in the murine BAL during the stages correlating with “peak viremia,” “inflammatory damage,” as well as the “recovery phase.” In addition to activation of acute phase responses, a distinct class of lung proteins including surfactant proteins was found to be depleted from the BAL coincident with their “appearance” in the serum, presumably due to leakage of the protein following loss of the integrity of the lung/epithelial barrier. Serum levels of at least two of these proteins were elevated in influenza patients during the febrile phase of infection compared to healthy controls or to the same patients at convalescence.Conclusions/SignificanceThe findings from this study provide a molecular description of disease progression in a mouse model of influenza and demonstrate its potential for translation into a novel class of markers for measurement of acute lung injury and improved case management.

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Section: Discussionsupporting

confidence: 91%

Molecular Analysis of Serum and Bronchoalveolar Lavage in a Mouse Model of Influenza Reveals Markers of Disease Severity That Can Be Clinically Useful in Humans

et al. 2014

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“…Comparison of plasma levels of cytokine/chemokine in A (H1N1) pdm09 influenza and seasonal H3 influenza revealed that dysregulated cytokines/chemokines production was higher in A (H1N1) pdm09 than seasonal H3 when compared to healthy controls. Similar to other previous studies, majority of the proinflammatory mediators of immune response tested in this study were present in higher levels in A (H1N1) pdm09 influenza . Plasma levels of proinflammatory cytokines, IL‐6 and IL‐17A were significantly higher in both A (H1N1) pdm09 influenza and seasonal H3 cases, with severe cases having the highest.…”

Section: Discussionsupporting

confidence: 89%

Proinflammatory chemokines are major mediators of exuberant immune response associated with Influenza A (H1N1) pdm09 virus infection

Thomas

Mani

Philip

et al. 2017

Journal of Medical Virology

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In India, the case fatality ratio of the pandemic A (H1N1) pdm09 influenza was relatively higher when compared to seasonal Influenza A infection. Hypercytokinemia or "cytokine storm" has been previously implicated in the pathogenesis of other influenza viruses. The present study was undertaken to compare the cytokine profiles of A (H1N1) pdm09 influenza and seasonal H3 infection in Indian population and to correlate the findings with disease severity. Plasma levels of 18 cytokines/chemokines were measured by flow-cytometry using a bead based assay in patients infected with A (H1N1) pdm09 virus (n = 96) and Influenza A seasonal H3 virus (n = 30) categorised into mild, moderate, and severe groups along with healthy controls (n = 36). There was an overall trend indicating an exuberant cytokine/chemokine response in A (H1N1) pdm09 as compared to seasonal H3 influenza, which was more evident in severe cases, suggesting a role for these cytokines/chemokines in the pathogenesis of A(H1N1) pdm09. Increased levels of CXCL-8/IL-8, IL-10, IL-6, and IL-17A were seen in both A(H1N1) pdm09 influenza and seasonal H3 cases when compared to healthy controls. However, dysregulated production of proinflammatory chemokines was seen more pronounced in A (H1N1) pdm09 influenza cases as compared to seasonal H3 cases. This study has brought forth the potential role of chemokines as prognostic indicators of disease severity and outcome. Further research on modulating the host immune response to limit severity of the disease could help in the treatment and management of influenza.

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“…Another study found that TGF-b blood levels correlated with the etiological agent of CAP (20). The finding that low serum levels of TGF-b correlate with influenza A (H1N1)pdm09 infection has been previously reported for another series, but it was, surprisingly, reported as not significant (27). Platelet levels in the blood are known to correlate with TGF-b when serum samples are obtained by venipuncture (7).…”

Section: Discussionmentioning

confidence: 79%

TGF-β Blood Levels Distinguish Between Influenza A (H1N1)pdm09 Virus Sepsis and Sepsis due to Other Forms of Community-Acquired Pneumonia

et al. 2015

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There is a strong interest in finding adequate biomarkers to aid in the diagnosis and prognosis of influenza A (H1N1)pdm09 virus infection. In this study, serum levels of inflammatory cytokines and laboratory markers were evaluated to assess their usefulness as biomarkers of influenza A (H1N1)pdm09 and their association with fatal cases. Serum samples of consecutive patients with a clinical presentation suggestive of influenza A (H1N1)pdm09 and progression to sepsis were evaluated. Serum inflammatory cytokines and routine laboratory tests were performed and correlated with positivity for influenza A (H1N1)pdm09 influenza by real time reverse transcription polymerase chain reaction and the results of three clinical severity scores (Sequential Organ Failure Assessment [SOFA], CURB-65, and Acute Physiology and Chronic Health Evaluation II [APACHE II]). High SOFA scores and some of its individual components, but not CURB-65 or APACHE II scores, correlate with fatal cases regardless of etiology. Total and unconjugated bilirubin, Ca + + , Cl -, prothrombin times, and partial thromboplastin times discriminate influenza A (H1N1)pdm09 from other causes of community-acquired pneumonia.

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Plasma cytokine levels and cytokine gene polymorphisms in Mexican patients during the influenza pandemic A(H1N1)pdm09

Cited by 36 publications

References 29 publications

Molecular Analysis of Serum and Bronchoalveolar Lavage in a Mouse Model of Influenza Reveals Markers of Disease Severity That Can Be Clinically Useful in Humans

Molecular Analysis of Serum and Bronchoalveolar Lavage in a Mouse Model of Influenza Reveals Markers of Disease Severity That Can Be Clinically Useful in Humans

Proinflammatory chemokines are major mediators of exuberant immune response associated with Influenza A (H1N1) pdm09 virus infection

TGF-β Blood Levels Distinguish Between Influenza A (H1N1)pdm09 Virus Sepsis and Sepsis due to Other Forms of Community-Acquired Pneumonia

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