Plasma Cytokines as Markers of Aseptic Prosthesis Loosening

Hundrić-Hašpl, Željka; Pećina, Marko; Hašpl, Miroslav; Tomičić, Maja; Jukić, Irena

doi:10.1097/01.blo.0000229365.57985.96

Cited by 28 publications

(34 citation statements)

References 31 publications

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“…Other studies described other conditions with significant levels of various cytokines in the synovial fluid of patients with rheumatoid arthritis and also in patients with osteoarthritis, supporting a pro-inflammatory process in the pathogenesis of osteoarthritis [28][29][30]. Elevated plasma levels of proinflammatory cytokines, such as IL-1beta, IL-8 and TNFalpha, were also observed as useful markers of bone resorption in patients with aseptic loosening of large joint prosthesis [31].…”

Section: Discussionmentioning

confidence: 82%

Cytokine Levels (IL-4, IL-6, IL-8 and TGFβ) as Potential Biomarkers of Systemic Inflammatory Response in Trauma Patients

Volpin

Cohen

Assaf

et al. 2014

International Orthopaedics (SICOT)

111

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Purpose Much research is now being conducted in order to understand the role of cytokines in the development of the inflammatory response following trauma. The purpose of this study was to evaluate whether serum levels of certain cytokines, measured immediately after initial injury, can be used as potential biomarkers for predicting the development and the degree of severity of the systemic inflammatory response (SIRS) in patients with moderate and severe trauma. Methods We conducted a prospective study with 71 individuals of whom 13 (18.3 %) were healthy controls and 58 (81.7 %) were traumatized orthopaedic patients who were categorized into two groups: 31 (43.6 %) with moderate injuries and 27 (38.1 %) patients with severe orthopaedic trauma. Thirty cc of heparinized blood were drawn from each individual within a few hours after the injury. Serum levels of pro-inflammatory, regulatory and anti-inflammatory cytokines were measured in each individual participant.

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Section: Discussionmentioning

confidence: 82%

Cytokine Levels (IL-4, IL-6, IL-8 and TGFβ) as Potential Biomarkers of Systemic Inflammatory Response in Trauma Patients

Volpin

Cohen

Assaf

et al. 2014

International Orthopaedics (SICOT)

111

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“…13,22,23 However, the role of pro-inflammatory cytokines in human disease has been less well characterized. Although there are several reports clearly demonstrating elevated levels of tumor necrosis factor alpha (TNF-a) in serum, joint synovial fluid, and periprosthetic tissues of osteolysis patients, [24][25][26][27] others have failed to observe such differences, with TNF-a levels in osteolysis patients being similar or lower than controls, or undetectable. [28][29][30][31][32] These conflicting findings raise the possibility that the involvement of macrophages in osteolysis may not be limited to pro-inflammatory cytokine signaling.…”

Section: Introductionmentioning

confidence: 99%

Expression profiling reveals alternative macrophage activation and impaired osteogenesis in periprosthetic osteolysis

Koulouvaris

Ivashkiv

et al. 2007

Journal Orthopaedic Research

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Interactions between periprosthetic cells and prosthetic wear debris have been recognized as an important event in the development of osteolysis and aseptic loosening. Although the ability of wear debris to activate pro-inflammatory macrophage signaling has been documented, the full repertoire of macrophage responses to wear particles has not been established. Here, we examined the involvement of alternative macrophage activation and defective osteogenic signaling in osteolysis. Using real-time RT-PCR analysis of periprosthetic soft tissue from osteolysis patients, we detected elevated levels of expression of alternative macrophage activation markers (CHIT1, CCL18), chemokines (IL8, MIP1 a) and markers of osteoclast precursor cell differentiation and multinucleation (Cathepsin K, TRAP, DC-STAMP) relative to osteoarthritis controls. The presence of cathepsin K positive multinuclear cells was confirmed by immunohistochemistry. Reduced expression levels of the osteogenic signaling components BMP4 and FGF18 were detected. Expression levels of TNF-a, IL-6, and RANKL were unchanged, while the anti-osteoclastogenic cytokine OPG was reduced in osteolysis patients, resulting in elevated RANKL:OPG ratios. In vitro studies confirmed the role of particulate debris in alternative macrophage activation and inhibition of osteogenic signaling. Taken together, these results suggest involvement in osteolysis of alternative macrophage activation, accompanied by elevated levels of various chemokines. Increased recruitment and maturation of osteoclast precursors is also observed, as is reduced osteogenesis. These findings provide new insights into the molecular pathogenesis of osteolysis, and identify new potential candidate markers for disease progression and therapeutic targeting. ß

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“…only a few minutes. Upon degradation, inactivated cytokine monomers are found in plasma, which can be detected several days after cessation of their biological activity [14,22]. Because of that we presumed that IL-1β could reach the peripheral circulation only in the patients who received ACS.…”

Section: Discussionmentioning

confidence: 99%

“…They postulated that the reference values set by the manufacturer were based on the mean value in a population sample, thus being of limited value. The individual levels of the tested substance may be detected only if they exceed the mean level of this substance determined in the respective population [11,14]. Therefore, we considered the plasma levels of IL-1 that were equal to or below the limit of test sensitivity as normal and those exceeding the limit of test sensitivity as increased levels.…”

Section: Discussionmentioning

confidence: 99%

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Correlation between synovial fluid and serum IL-1β levels after ACL surgery–preliminary report

Daraboš

Hundrić-Hašpl

Hašpl

et al. 2008

International Orthopaedics (SICOT)

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The possibility of controlling the harmful intraarticular influence of elevated interleukin (IL)-1β synovial fluid concentration after anterior cruciate ligament (ACL) surgery could be useful. We investigated the correlation between serum and synovial fluid IL-1β levels following ACL reconstruction. We measured IL-1β concentration periodically in three synovial fluid and four serum samples in each of 20 patients receiving either autologous conditioned serum (ACS) containing endogenous anti-inflammatory cytokines including IL-1Ra and several growth factors (group A) or placebo (group B). A decrease in IL-1β synovial fluid concentration appeared to be more pronounced in absolute terms in group A. In eight patients serum IL-1β was detected on the 6th postoperative day. In four of them whose synovial fluid levels were over 10 pg/ml on the 6th postoperative day, serum IL-1β was detected on the 10th postoperative day. The results were different in group B. Correlation between serum and synovial fluid IL-1β appearance persists in patients after ACL surgery and ACS application. This study is an example of ACS influence on the ACL healing process controlling the IL-1β levels on the basis of the serum IL-1β detection.

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Plasma Cytokines as Markers of Aseptic Prosthesis Loosening

Cited by 28 publications

References 31 publications

Cytokine Levels (IL-4, IL-6, IL-8 and TGFβ) as Potential Biomarkers of Systemic Inflammatory Response in Trauma Patients

Cytokine Levels (IL-4, IL-6, IL-8 and TGFβ) as Potential Biomarkers of Systemic Inflammatory Response in Trauma Patients

Expression profiling reveals alternative macrophage activation and impaired osteogenesis in periprosthetic osteolysis

Correlation between synovial fluid and serum IL-1β levels after ACL surgery–preliminary report

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