Plasma, erythrocyte membrane bound enzymes and tissue histopathology in male Wistar rats exposed to common insecticides

Nwozo, Sarah Onyenibe; Akpodono, Enor; Oyinloye, Babatunji Emmanuel

doi:10.1584/jpestics.d14-065

Cited by 5 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, significant decrease in body weight gain recorded in mice administrated CBZ. Previous studies reported that the decrease in body weight might be due to the decrease in food consumption and/ or increase degradation of protein and lipids ( Bailey et al, 2004 , Mansour and Mossa, 2010 , Nwozo et al, 2015 ). Administration of CBZ resulted in significant decrease of RBC, Hb, Hct and WBC values.…”

Section: Discussionmentioning

confidence: 99%

Effect of Nigella sativa and Foeniculum vulgare seeds extracts on male mice exposed to carbendazim

Alghamdi

2020

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

The increasing prevalence of environmental pollutants such as pesticides is a major global problem that affects living organisms. Exposure to environmental pollutants remains a major source of health risk throughout the world. The potential health benefits of various medicinal plants and natural products in relation to protect various diseases are currently receiving considerable attention. A current approach is to develop a new biological compound from natural products that inhibits pain. Ethnopharmacological surveys have been found to be one of the most reliable tools for the discovery of the natural and semi-synthetic drug. The present study was performed to investigate the hematological and biochemical changes induced by carbendazim (CBZ) and the potential protective effect of seeds extracts of Nigella sativa (NSSE) and Foeniculum vulgare (FVSE) against CBZ toxicity in male mice. Mice were distributed into 6 groups. Mice of group 1 were served as control. Group 2 was exposed to CBZ. Group 3 was supplemented with NSSE and exposed to CBZ. Group 4 was treated with FVSE and CBZ. Normal mice of group 5 and 6 were subjected to NSSE and FVSE respectively. Body weight gain was significantly decreased in mice of group 2. In mice of group 2, significant declines of RBC, HB, Hct, WBC, total protein, FSH, LH, testosterone, T4, T3, CAT and SOD were observed. Moreover, the levels of ALT, AST, ALP, total bilirubin, creatinine, BUN, uric acid, glucose, cholesterol, CK, LDH, MDA and GSH were significantly enhanced. Treatment with NSSE and FVSE showed attenuation effects against CBZ induced hematological and biochemical changes. The results suggest that the attenuation effects of NSSE and FVSE attributed to their antioxidant properties.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Nigella sativa and Foeniculum vulgare seeds extracts on male mice exposed to carbendazim

Alghamdi

2020

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…Recent literature data also suggest, that neonicotinoids affect probably far more taxa and exert much wider ecological effects than was expected since this third generation of insecticides was introduced Simon-Delso et al, 2015). Accumulating data demonstrate that neonicotinoid effects are not confined in the CNS, but potentially act more systematically and also induce intracellular alterations, both in vertebrates and invertebrates, (Nwozo et al, 2015;Brandt et al, 2016;Kataria et al, 2016;Sheets et al, 2016;Kudelska et al, 2017;Tavares, et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Neonicotinoid insecticides are potential substrates of the multixenobiotic resistance (MXR) mechanism in the non-target invertebrate, Dreissena sp.

et al. 2018

View full text Add to dashboard Cite

Mussels are among the most frequently used invertebrate animals in aquatic toxicology to detect toxic exposure in the environment. The presence and activity of a cellular defence system, the multixenobiotic resistance (MXR) mechanism, was also established in these organisms.In isolated gill tissues of dreissenid mussels (D. bugensis) the MXR activity was assayed after treatment by commercially available insecticides (formulated products) which contain neonicotinoids as their active ingredients: Actara (thiamethoxam), Apacs (clothianidin), Calypso (thiacloprid) and Kohinor (imidacloprid), respectively. While applying the accumulation assay method, 0.5 μM rhodamine B was used as model substrate and 20 μM verapamil as model inhibitor of the MXR mechanism.In acute (in vitro) experiments when isolated gills were co-incubated in graded concentrations of insecticides and rhodamine B simultaneously, Calypso and Kohinor treatment resulted increasing rhodamine accumulation. Chemical analysis of gills in vitro incubated in insecticides demonstrated higher tissue concentrations of thiamethoxam, clothianidin and thiacloprid in the presence of verapamil suggesting that the active ingredients of Actara, Apacs and Calypso are potential substrates of the MXR mediated cellular efflux. In contrast, verapamil did significantly alter the accumulated imidacloprid concentrations in gills, suggesting that the active component of Kohinor is not transported by the MXR mechanism.Chronic (in vivo) exposures of the intact animals in lower, 1, 10 mg/L concentration of neonicotinoid products, resulted in a decreased level of both rhodamine accumulation and verapamil inhibition by the 12 th -14 th days of treatment. These results suggest an enhancement of MXR activity (chemostimulation), building up gradually in the animals exposed to Actara, Apacs and Kohinor, respectively.Neonicotinoid-type insecticides are generally considered as selective neurotoxins for insects, targeting the nicotinic type acetylcholine receptors (nAChRs) in their central nervous system. Our present results provide the first evidences that neonicotinoid insecticides are also able to alter the transmembrane transport mechanisms related to the MXR system.

show abstract

“…This synthetic pyrethroid monoizomer of significant toxicity poses a real threat of intoxication when application standards violated. Despite numerous studies on pyrethroid toxicology [4][5][6], the pathogenesis of acute toxicity due to these products, the principles of laboratory diagnosis and therapy in the early stages of intoxication require in-depth study.…”

Section: Introductionmentioning

confidence: 99%

Adaptive and Compensatory Responses in Rats at the Early Stages of an Acute Intoxication With Deltamethrin

Gerunov¹,

Чигринский²,

Fedorov³

et al. 2016

S-h. biol.

View full text Add to dashboard Cite

A b s t r a c tThe world market of synthetic pyrethroids is estimated more than at 2.5 bn. dollars and will grow in the next years. Numerous works of domestic and foreign authors are devoted to toxicology of pyrethroids, however the issues related to pathogenesis of an acute poisoning of animals with pesticides of that group as well as the principles of laboratory diagnostics at an early stage of intoxication and pathogenetic therapy at poisoning warrant further investigations. For many years deltamethrin has been successfully used in plant growing as well as in animal husbandry creating a poisoning hazard in case of violation of treatment regulations. Morbidity and clinical outcome depend largely on severity of functional endocrine and immune systems disorders. The purpose of the study was to determine dynamics of adaptive and compensatory reactions in animals at an early stage of an acute intoxication with deltamethrin. In experiment white laboratory rats as an established mammal model in biomedical research were used. The experiment was performed on male rats (weight of 180-200 gr) arranged in 6 groups (of 10-12 rats each). Animals from groups II, IV and VI have been subjected to an acute peroral intoxication with deltamethrin (Butox 50, Intervet, Netherlands) in a dose of 43.5 mg/kg of body weight. Rats of groups I, III and V served as control. Rats from different groups were put out of the experiment sequentially: I and II -in a day; III and IV -in three days; V and VIin a week after the beginning of the experiment. Glucose content was estimated in whole blood of rats, and concentration of insulin and corticosterone was assayed in blood serum. Pieces of animal timus were fixed in 4 % neutral formaldehyde, dehydrated in alcohols with the increasing concentration and embedded into paraffin. Histologic sections at 3-5 microns in thickness were made with the rotational microtome and stained with haematoxylin and eosin, and also according to Van-Giezon. For identification of mast cells the histologic sections were stained with Bismarck brown to Shubich. Increase in blood corticosterone, insulin and glucose levels by 46.3 (p = 0.0001), 31.9 (p = 0.0139) and 25.6 % (p = 0.0052), respectively, was found in a day after poisoning with deltamethrin. On day 3 after poisoning the blood concentration of corticosterone and glucose in rats remained high along with a decrease in insulin content. Hypocorticosteronemia and hypoglycemia were observed on day 7 after poisoning, with the insulin level close to control values. Corticosterone and glucose content has been reduced by 17.3 (p = 0.0407) and 19.8 % (p = 0.0267), respectively, compared to control. Photomicrographs showed a reduction in the number of thymocytes, activation of apoptosis, an increase in quantity of mastocytes along with an intensification of degranulation as well as development of haemodynamic disorders in thymus when poisoning rats. In a week after intoxication the thymocytes level was partially restored. No animal died during experiment which evidences th...

show abstract

Plasma, erythrocyte membrane bound enzymes and tissue histopathology in male Wistar rats exposed to common insecticides

Cited by 5 publications

References 27 publications

Effect of Nigella sativa and Foeniculum vulgare seeds extracts on male mice exposed to carbendazim

Effect of Nigella sativa and Foeniculum vulgare seeds extracts on male mice exposed to carbendazim

Neonicotinoid insecticides are potential substrates of the multixenobiotic resistance (MXR) mechanism in the non-target invertebrate, Dreissena sp.

Adaptive and Compensatory Responses in Rats at the Early Stages of an Acute Intoxication With Deltamethrin

Contact Info

Product

Resources

About