Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC–MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Giton, Frank; Caron, Patrick; Bérubé, René; Bélanger, Alain; Barbier, Olivier; Fiet, Jean

doi:10.1016/j.cca.2010.04.022

Cited by 25 publications

(17 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A heavy isotope internal standard for estrone-3-sulfate (2,4,16,16-[ 2 H 4 ]-estrone-3-sulfate) is commercially available from C/D/N isotopes (Pointe-Claire, CA) so that reliable quantitative assays can be conducted for this important analyte [22,55]. It is surprising that this standard has not generally been used for quantitative determinations of estrone-3-sulfate in the serum of postmenopausal women.…”

Section: Conjugated Estrogensmentioning

confidence: 99%

“…It is surprising that this standard has not generally been used for quantitative determinations of estrone-3-sulfate in the serum of postmenopausal women. When conducting quantitative determinations, care has to be taken with calibration standards because both unlabeled and labeled estrone-3-sulfate contain Tris as a stabilizer as well as significant amounts of water [55]. Quantifying the actual amount of estrone-3-sulfate in solutions requires UV spectrophotometry, in which the λ max of estrone at 270 nM (ε 2000) is measured [56].…”

Section: Conjugated Estrogensmentioning

confidence: 99%

“…However, specific analyses of estrone-sulfate (137 to 440 pg/mL) and estrone-3β-glucuronide (22.6 to 33.9 pg/mL) suggest that some of the total estrone conjugate values obtained after G/S hydrolysis might be an underestimate of the true values (Table 2). Therefore, future studies should focus on the analysis of intact estrone-sulfate using stable isotope LC-SRM/MS methodology [22,55]. This approach would be particularly useful for monitoring the effect of aromatase inhibitors [60].…”

Section: Conjugated Estrogensmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of estrogens and androgens in postmenopausal serum and plasma by liquid chromatography–mass spectrometry

et al. 2015

View full text Add to dashboard Cite

Liquid chromatography-selected reaction monitoring/mass spectrometry-based methodology has evolved to the point where accurate analyses of trace levels of estrogens and androgens in postmenopausal serum and plasma can be accomplished with high precision and accuracy. A suite of derivatization procedures has been developed, which together with modern mass spectrometry instrumentation provide investigators with robust and sensitive methodology. Preionized derivatives are proving to be useful as they are not subject to suppression of the electrospray signal. Postmenopausal women with elevated plasma or serum estrogens are thought to be at increased risk for breast and endometrial cancer. Therefore, significant advances in risk assessment should be possible now that reliable methodology is available. It is also possible to conduct analyses of multiple estrogens in plasma or serum. Laboratories that are currently employing liquid chromatography/mass spectrometry methodology can now readily implement this strategy. This will help conserve important plasma and serum samples available in Biobanks, as it will be possible to conduct high sensitivity analyses using low initial sample volumes. Reported levels of both conjugated and non-conjugated estrogen metabolites are close to the limits of sensitivity of many assays to date, urging caution in the interpretation of these low values. The analysis of serum androgen precursors in postmenopausal women has not been conducted routinely in the past using liquid chromatography/mass spectrometry methodology. Integration of serum androgen levels into the panel of metabolites analyzed could provide additional information for assessing cancer risk and should be included in the future.

show abstract

Section: Conjugated Estrogensmentioning

confidence: 99%

Section: Conjugated Estrogensmentioning

confidence: 99%

Section: Conjugated Estrogensmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of estrogens and androgens in postmenopausal serum and plasma by liquid chromatography–mass spectrometry

et al. 2015

View full text Add to dashboard Cite

show abstract

“…DHEA, D4-androstenedione (4-dione), D5-androstenediol (5-diol), testosterone (TT), dihydrotestosterone (DHT), estrone (E1), estradiol (E 2 ), and E1 sulfate (E1-S) were assayed simultaneously by MS coupled with GC on 1000 ml serum (20,22,23). Briefly, deuterated steroid internal standards (CDN Isotopes, Inc., Point-Claire, Quebec, Canada) were added to all serum samples and extracted with 1-chlorobutane.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Serum sex steroids measured in middle-aged European and African–Caribbean men by gas chromatography–mass spectrometry

Giton

Fiet

Cornu³

et al. 2011

European Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

Background: Differences in circulating steroid hormone levels have been hypothesized to explain ethnic differences in steroid-related diseases. The aim of this study was to determine the serum levels of a wide panel of steroid hormones, both androgens and estrogens, in healthy middle-aged African-Caribbean and European men. Design and methods: Serum steroid hormone levels were determined in men participating in a systematic public health study funded by the French National Health Insurance system. Blood was collected in the morning from 304 healthy African-Caribbean and European men aged between 40 and 69 years. Serum steroids were measured by mass spectrometry-gas chromatography, except for DHEAS and sex hormone-binding globulin, which were determined by RIA. Data were analyzed in 10-year age intervals by analysis of covariance, with adjustment for age, body mass index, waist-to-hip ratio, tobacco and alcohol consumption, and season of sampling. Results: Compared with Europeans, African-Caribbean men presented significantly higher serum levels of measured bioavailable testosterone, 4-androstenedione (4-dione), and estrone (E1) regardless of the age group, of 5-androstenediol (5-diol) in those aged 40-49 and 50-59 years, and of testosterone (TT) and dihydrotestosterone in those aged 40-49 years. In contrast, European men aged 40-69 years showed significantly higher serum levels of DHEA and DHEAS. Conclusions: Significant differences in serum steroid hormone levels were observed in middle-aged African-Caribbean and European men. Whether such differences could contribute to ethnic differences in disease risk in adult men remains to be investigated. Some steroids, such as bioavailable TT, 4-dione, 5-diol, and E1, deserve particular attention.

show abstract

“…Recently, GC-MS/MS has been used for the analysis of marker metabolites such as those related to alcohol abuse in human hair [127e129], 4-aminobiphenyl hemoglobin adducts in smokers and nonsmokers [130], estrone sulfate in human plasma [131], endogenous anabolic steroids in human hair [132], endogenous organic acids in human urine [133], tyrosine metabolites in human urine [134], plasma [135], exhaled breath [136], and pyrimidine metabolites in human urine [137]. Thus, an increasing trend for using GC-MS/MS for targeted profiling and 24.6.…”

Section: Future Directionsmentioning

confidence: 99%

Metabonomics

Chan¹,

Mal²,

Pasikanti³

2012

Gas Chromatography

View full text Add to dashboard Cite

Plasma estrone sulfate assay in men: Comparison of radioimmunoassay, mass spectrometry coupled to gas chromatography (GC–MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Cited by 25 publications

References 23 publications

Analysis of estrogens and androgens in postmenopausal serum and plasma by liquid chromatography–mass spectrometry

Analysis of estrogens and androgens in postmenopausal serum and plasma by liquid chromatography–mass spectrometry

Serum sex steroids measured in middle-aged European and African–Caribbean men by gas chromatography–mass spectrometry

Metabonomics

Contact Info

Product

Resources

About