Plasma Fluorescent Oxidation Products and Bone Mineral Density Among Male Veterans: A Cross-Sectional Study

Shen, Xue; Peng, Chuangang; Zhao, Yan; Zhong, Lili; Cai, Hanqing; Kan, Bo; Zhang, Yuzheng; Xue, Shanshan; Qiao, Wenjing; Zhao, Qianqian; Guo, Dingjie; Li, Binbin; Vuong, Ann M.; Yang, Shuman

doi:10.1016/j.jocd.2021.09.003

Cited by 7 publications

(10 citation statements)

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“…As far as we know, this is the first study examining the relationship between FlOPs and QUS parameters. To some extent, our results were in agreement with two previous studies, in which higher FlOP levels are associated with lower BMD in male veterans (20) and an increased risk of hip fracture in postmenopausal women (21). Since QUS reflects the structural and mechanical properties of the bone (22,23), the present study extended the evidence about the relationship between FlOPs and bone health in males and females.…”

Section: Discussionsupporting

confidence: 92%

“…Compared to specific oxidation measurements such as MDA assay via colorimetric thiobarbituric acid, the fluorescence method is 10-100 times more sensitive (19). In our previous study, higher FlOP levels were found to be associated with lower hip BMD in male veterans (20) and an increased risk of hip fracture in postmenopausal women (21). However, whether FlOPs are associated with bone health determined by QUS parameters is unclear.…”

Section: Introductionmentioning

confidence: 92%

“…We measured FlOPs for all individuals with collected blood samples. Measurement of FlOPs was performed based on the method modified by Shimasaki (25) and Wu (26), and has been previously described (20). In brief, plasma was extracted with ethanol/ether (3:1, v/v) and centrifuged at 3,000 rpm for 10 min at 4 • C. Then the supernatant was added to a black 96-well Microplate (Black Fat Bottom Polystyrene High Bind Microplate 3925, Corning) and measured by a fluorescent microplate reader (Cytation 3 Cell Imaging Multi-Mode Reader, BioTek, Vermont, USA) at a wavelength of 320/420 nm (excitation and emission wavelength).…”

Section: Flop Measurementmentioning

confidence: 99%

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Association between global biomarker of oxidative stress and quantitative ultrasound parameters in middle-aged and elderly adults: A cross-sectional study

Shen

Liu²,

Zhao³

et al. 2023

Front. Public Health

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IntroductionWith the population aging, osteoporosis has become a major public health concern. Elevated oxidative stress is a vital detrimental factor for bone health. Compared to common oxidative stress-related biomarkers, Fluorescent Oxidation Products (FlOPs) reflect the global levels of oxidation from proteins, lipids, and DNA. Nevertheless, whether plasma FlOP levels are related to bone health measured by Quantitative ultrasound (QUS) is unclear. Thus, the present study examined the association between FlOPs and QUS parameters in middle-aged and elderly adults.MethodsThis community-based cross-sectional study was conducted in Changchun, northeast China. Plasma FlOPs were determined by a fluorescent microplate reader at a wavelength of 320/420 nm (excitation/emission). QUS parameters [speed of sound (SOS) and broadband ultrasound attenuation (BUA)] of the calcaneus were assessed by an ultrasound bone densitometer. We used multivariable linear regression to examine the association between FlOPs and QUS parameters.ResultsA total of 491 subjects were included in this study. Their average age was 65.2 years (standard deviation [SD]: 9.7 years). FlOPs were inversely associated with SOS (β for an increase of logarithmic interquartile range = −10.64; P = 0.018). Higher FlOP levels were marginally associated with lower SOS in females (β for an increase of logarithmic interquartile range = −9.68, P = 0.066), but not in males (β for an increase of logarithmic interquartile range = −11.84, P = 0.131). No significant relationship between FlOPs and BUA was observed.ConclusionsPlasma FlOP levels were inversely associated with SOS, but not with BUA in middle-aged and elderly adults.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 92%

Section: Flop Measurementmentioning

confidence: 99%

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Association between global biomarker of oxidative stress and quantitative ultrasound parameters in middle-aged and elderly adults: A cross-sectional study

Shen

Liu²,

Zhao³

et al. 2023

Front. Public Health

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“…Levels of FlOPs in plasma were measured by a modified Shimasaki's method, which was previously applied by our previous study (2). In brief, we extracted plasma with ethanol/ether (3:1, v/v) and with supernatant added for the detecting instrument.…”

Section: Measurement Of Oxidative Stress Biomarkersmentioning

confidence: 99%

“…Oxidative stress is characterized as an insufficient endogenous antioxidant defense system against the overproduction of reactive oxygen species (ROSs) (1). A high level of oxidative stress causes damage to lipids, proteins, and DNA, which leads to an increased risk of many chronic conditions, such as cardiovascular disease, cancer, chronic kidney disease, and osteoporosis (2)(3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

Postprandial changes of oxidative stress biomarkers in healthy individuals

Huang

Shen²,

Zhang³

et al. 2022

Front. Nutr.

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Food consumption induces oxidative stress in humans, but the changes in oxidative stress levels after a regular meal are still unclear. We conducted an experimental study on 20 healthy volunteers (10 males, 10 females), who matched in age (±2 years). They were given a regular diet (total energy of 704 kcal, which contains 75 g of carbohydrates, 35 g of protein, and 29 g of lipids) at 11:30 a.m. after a fast of over 12 h. We collected 6-repeated measures of venous blood samples at 2-h intervals via heparin anticoagulant tubes immediately after the meal (indicated as “0” h) and up to 10 h post-consumption. Biomarkers included plasma fluorescent products, plasma malondialdehyde, plasma total antioxidant capacity, and plasma superoxide dismutase. FlOPs were measured at three excitation/emission wavelengths (FlOP_320, FlOP_360, and FlOP_400). The average age and BMI for the twenty participants were 22.70 ± 1.98 years and 20.67 ± 2.34 kg/m2, respectively. Within 10 h after the meal, the overall trend of FlOPs were generally similar. There was no evidence of dose response for any of the three FlOPs (all P > 0.05). However, levels of MDA decreased with the time of fasting (Plinear and Pquadratic < 0.05), with the biggest decrease occurring between 0 and 2 h post-meal. The overall trend of T-AOC and SOD levels also decreased with fasting time (Plinear and Pquadratic < 0.05), though an increase was observed between 0 and 2 h following consumption. Levels of MDA, T-AOC, and SOD but not FlOPs, decreased with fasting time.

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Next-generation analytical platforms for antioxidant capacity assessment: The urge for realistic and physiologically relevant methods

Granato

2023

Biomedicine & Pharmacotherapy

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Plasma Fluorescent Oxidation Products and Bone Mineral Density Among Male Veterans: A Cross-Sectional Study

Cited by 7 publications

References 55 publications

Association between global biomarker of oxidative stress and quantitative ultrasound parameters in middle-aged and elderly adults: A cross-sectional study

Association between global biomarker of oxidative stress and quantitative ultrasound parameters in middle-aged and elderly adults: A cross-sectional study

Postprandial changes of oxidative stress biomarkers in healthy individuals

Next-generation analytical platforms for antioxidant capacity assessment: The urge for realistic and physiologically relevant methods

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