Plasma free metanephrine and normethanephrine levels correlated to plasma catecholamine after acute running in amateur runner

Abstract

Background Catecholamine is a typical index of exercise intensity, but it is difficult to detect. Plasma metanephrine (MN) and normethanephrine (NMN) levels are more stable than those of catecholamines. This study aimed to investigate plasma MN and NMN levels during acute exercise running in amateur runners. Methods Samples were collected from eight healthy male participants. They were either sedentary or running at low or high intensity for 30 min. Blood samples were collected under these conditions. Measurements taken included plasma adrenaline, noradrenaline, MN, and NMN. Results Plasma adrenaline levels increased after high-intensity exercise compared with sedentary subjects. Plasma noradrenaline, MN, and NMN levels increased after both low- and high-intensity exercise compared with sedentary subjects. In addition, these levels were also significantly higher at high intensity than at low intensity. Plasma adrenaline and noradrenaline levels were positively correlated with plasma free MN and NMN levels after acute running, respectively. Conclusion This study revealed that plasma MN and NMN levels transiently increased depending on exercise intensity in amateur runners. In addition, plasma NMN levels are better markers than plasma MN levels because of their stronger correlation with plasma catecholamine levels.

Acute Hemodynamic, Metabolic, and Hormonal Responses to a Boxing Exergame with and without Blood Flow Restriction in Non-Athlete Young Individuals

“…Moreover, a session of aerobic exercise significantly reduces blood sugar levels (glucose) compared with resistance exercise in women with type 2 diabetes [ 17 ] and provides a stimulus for the release of growth hormone (GH) [ 18 ]. Physical stressors, such as sport-related activities, stimulate the release of normetanephrine at all workloads potentially indicating a method of determining exercise intensity and exercise performance [ 19 ]. In contrast, the release of epinephrine increases during workloads greater than 61% of VO 2max , which, in part, may be responsible for the increased HR and blood pressure (BP) responses [ 20 ].…”

Selective Separation and Analysis of Catecholamines in Urine Based on Magnetic Solid Phase Extraction by Mercaptophenylboronic Acid Functionalized Fe3O4-NH2@Au Magnetic Nanoparticles Coupled with HPLC

“…In spite of the great diversity of analytical approaches that have been developed in recent years, the detection of catecholamines in biological samples remains a hot spot in analytical fields [7,8]. High-performance liquid chromatography (HPLC) is an analytical method routinely used for the separation and quantification of catecholamines in clinical laboratories [9,10], usually coupled with electrochemical [11], fluorescence [12] or mass spectrometry detection [13]. Among the detectors, electrochemical detectors [14,15] attract more preference due to good selectivity, highly sensitive characteristics, a lack of need for derivatization and low detection cost.…”