Plasma from the second and third weeks after open colorectal resection for cancer stimulates in vitro endothelial cell growth, migration, and invasion

Kumara, H. M. C. Shantha; Kirchoff, Daniel D.; Naffouje, Samer A.; Grieco, Michael J.; Herath, Sonali A.; Dujovny, Nadav; Kalady, Matthew F.; Hyman, Neil; Njoh, Linda; Whelan, Richard L.

doi:10.1007/s00464-011-1953-1

Cited by 14 publications

(36 citation statements)

References 7 publications

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“…It is also possible that IL-8, via different mechanisms, may encourage tumor angiogenesis (18,19). There is EC in vitro cell culture evidence that plasma from the second and third weeks after MICR or open surgery for CRC stimulates EC's to proliferate, migrate, and invade at a significantly higher rate when compared to results from EC cultures into which the same patients preoperative plasma was added (12,17). Those results suggest that the net effect of the sustained elevations of IL-8 and the other proangiogenic proteins may encourage angiogenesis during the early postoperative period.…”

Section: Discussionmentioning

confidence: 99%

“…It has also been shown via in vitro endothelial cell (EC) culture studies that plasma from weeks 2 and 3 after MICR significantly stimulates endothelial cells to proliferate, migrate, and invade when compared to EC culture results obtained with the same patients preoperative plasma (12,17); these EC activities are critical to angiogenesis. It is possible, then, that the long duration proangiogenic plasma protein elevations may promote angiogenesis in residual tumor deposits left behind after resection of the primary tumor.…”

Section: Introductionmentioning

confidence: 99%

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Plasma interleukin-8 levels are persistently elevated for 1ï¿½month after minimally invasive colorectal resection for colorectal cancer

Kumara¹,

Sutton²,

Bellini³

et al. 2017

mol clin onc

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Abstract. Minimally invasive colorectal resection (MICR) for colorectal cancer (CRC) is associated with elevated levels of seven proangiogenic proteins that persist for 2-4 weeks after surgery. The proangiogenic plasma may promote tumor growth postoperatively in patients with residual cancer. To the best of our knowledge, the impact of surgery on interleukin 8 (IL-8) levels is unknown. The aim of the present study was to evaluate plasma IL-8 levels after MICR for CRC. Patients with CRC enrolled in an institutional review board-approved plasma/data bank who underwent MICR were eligible. Blood samples were taken preoperatively (preop) and at multiple postoperative (postop) time points, and were stored at -80˚C. Only patients for whom preop, postop day (POD) 1, POD 3 and at least 1 late postop plasma samples (POD7-34) available were enrolled. Clinical, demographical and pathological data were collected. IL-8 levels were determined via ELISA and results were reported as the mean and ± standard deviation. The Wilcoxon signed rank test was used for analysis with P<0.05 used as the significance threshold. A total of 73 CRC patients (colon, 62%; rectal, 38%) who underwent MICR (laparoscopic-assisted, 60%; hand-assisted, 40%) were studied. The mean preop IL-8 level was 20.4±10.6 pg/ml. Significant elevations in plasma IL-8 levels were noted compared with preop levels on POD1 (43.1±38.6; n=72; P<0.0001), POD 3 (33.0±30.1; n=71; P<0.0001), POD7-13 (29.9±21.9; n=50; P<0.0001), n=24; P=0.002), and for the POD21-27 time point (24.0±9.2; n=16; P= 0.002). In conclusion, plasma IL-8 levels were significantly elevated from baseline for 4 weeks after MICR for CRC. In conjunction with the other proangiogenic MICR-associated blood compositional changes, increased IL-8 levels may promote tumor angiogenesis and growth postop.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Plasma interleukin-8 levels are persistently elevated for 1ï¿½month after minimally invasive colorectal resection for colorectal cancer

Kumara¹,

Sutton²,

Bellini³

et al. 2017

mol clin onc

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“…Furthermore, human plasma from the second and third weeks after surgery has been shown to promote endothelial cell (EC) proliferation, invasion, and migration, which are key steps in angiogenesis [14,19] . It is possible, therefore, that the postoperative (PostOp) plasma composition may encourage the growth of residual tumor metastases after "curative" colorectal resection of a primary tumor.…”

Section: Introductionmentioning

confidence: 99%

Plasma chitinase 3-like 1 is persistently elevated during first month after minimally invasive colorectal cancer resection

Kumara

Gaita

Miyagaki

et al. 2016

WJGO

Self Cite

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AIM:To assess blood chitinase 3-like 1 (CHi3L1) levels for 2 mo after minimally invasive colorectal resection (MICR) for colorectal cancer (CRC). METHODS: CRC patients in an Institutional ReviewBoard approved data/plasma bank who underwent elective MICR for whom preoperative (PreOp), early postoperative (PostOp), and 1 or more late PostOp samples [postoperative day (POD) 7-27] available were included. Plasma CHi3L1 levels (ng/mL) were determined in RESULTS:PreOp and PostOp plasma sample were available for 80 MICR cancer patients for the study. The median PreOp CHi3L1 level was 56.8 CI: 41.9-78.6 ng/ mL (n = 80). Significantly elevated (P < 0.001) median plasma levels (ng/mL) over PreOp levels were detected on POD1 (667.7 CI: 495.7, 771.7; n = 79), POD 3 (132.6 CI: 95.5, 173.7; n = 76), 136.9; n = 62), 287.4; n = 22), 137.4; n = 20, P = 0.001).No significant difference in plasma levels were noted on POD27-41.CONCLUSION: Plasma CHi3L1 levels were significantly elevated for one month after MICR. Persistently elevated plasma CHi3L1 may support the growth of residual tumor and metastasis.

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“…Of these, five papers were included in the study ( Fig. 2 ) ( Kumara et al, 2009 ; Shantha Kumara et al, 2012 ; Salvans et al, 2014 ; Xu et al, 2016 ; Shantha Kumara et al, 2009 ). The last study was excluded as the patient group consisted of patients undergoing surgery for either colorectal cancer or gastric bypass and was therefore not a homogeneous group of patients undergoing surgery for colorectal cancer ( Kirman et al, 2002 ).…”

Section: Resultsmentioning

confidence: 99%

Assessment of perioperative stress in colorectal cancer by use of in vitro cell models: a systematic review

Kirkegaard

Gögenur

Gögenür

2017

PeerJ

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BackgroundThe perioperative period is important for patient outcome. Colorectal cancer surgery can lead to metastatic disease due to release of disseminated tumor cells and the induction of surgical stress response. To explore the overall effects on surgically-induced changes in serum composition, in vitro model systems are useful.MethodsA systematic search in PubMed and EMBASE was performed to identify studies describing in vitro models used to investigate cancer cell growth/proliferation, cell migration, cell invasion and cell death of serum taken pre- and postoperatively from patients undergoing colorectal tumor resection.ResultsTwo authors (MG and TK) independently reviewed 984 studies and identified five studies, which fulfilled the inclusion criteria. Disagreements were solved by discussion. All studies investigated cell proliferation and cell invasion, whereas three studies investigated cell migration, and only one study investigated cell death/apoptosis. One study investigated postoperative peritoneal infection due to anastomotic leak, one study investigated mode of anesthesia (general anesthesia with volatile or intravenous anesthetics), and one study investigated preoperative intervention with granulocyte macrophage colony stimulating factor (GMCSF). In all studies an increased proliferation, cell migration and invasion was demonstrated after surgery. Anesthetics with propofol and intervention with GMCSF significantly reduced postoperative cell proliferation, whereas peritoneal infection enhanced the invasive capability of tumor cells.ConclusionThis study suggests that in vitro cell models are useful and reliable tools to explore the effect of surgery on colorectal cancer cell proliferation and metastatic ability. The models should therefore be considered as additional tests to investigate the effects of perioperative interventions.

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Plasma from the second and third weeks after open colorectal resection for cancer stimulates in vitro endothelial cell growth, migration, and invasion

Cited by 14 publications

References 7 publications

Plasma interleukin-8 levels are persistently elevated for 1ï¿½month after minimally invasive colorectal resection for colorectal cancer

Plasma interleukin-8 levels are persistently elevated for 1ï¿½month after minimally invasive colorectal resection for colorectal cancer

Plasma chitinase 3-like 1 is persistently elevated during first month after minimally invasive colorectal cancer resection

Assessment of perioperative stress in colorectal cancer by use of in vitro cell models: a systematic review

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