Plasma heparin: A simple, one-sample determination of both effect and concentration based on the activated partial thromboplastin time

Hoffmann, J. J. M. L.; Meulendijk, P.N.

doi:10.1016/0009-8981(78)90187-0

Cited by 10 publications

(7 citation statements)

References 10 publications

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“…rhe heparin concentrarion by means of the AprrHN at g00 u /r. Hoffmann aná Meulendijk [2] had the same problem as can be deduced from their Fig. 28.…”

Section: Discussionmentioning

confidence: 66%

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Interindividual variation in relationships between plasma heparin concentration and the results of five heparin assays

Putten¹,

Ruit²,

Beunis³

et al. 1982

Clinica Chimica Acta

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In order to determine their value for estimating the heparin concentration in plasma, we established the relationship between test result and heparin concentration in plasma from various individuals, for five assays used with heparin treatment. Only assays which can be carried out routinely in clinical laboratories were considered. The thrombin time and the whole blood recalcification time give pointless and ambiguous information respectively, concerning the heparin level. The activated partial thromboplastin time with and without heparin neutralisation give only a rough estimate. The spectrophotometric method using a chromogenic substrate gives the best information. The latter can be improved by using a non-linear (parabolic) equation for the calculation of the reference curve. Current heparin therapy, controlled with the aid of a clotting assay, may result in plasma heparin concentrations that vary widely from one patient to another.

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“…rhe heparin concentrarion by means of the AprrHN at g00 u /r. Hoffmann aná Meulendijk [2] had the same problem as can be deduced from their Fig. 28.…”

Section: Discussionmentioning

confidence: 66%

“…This was performed as described by Hoffmann and Meulendijk [2]. The APTT was repeated after the heparin level had been increased by a known value.…”

Section: Apttnhmentioning

confidence: 99%

Interindividual variation in relationships between plasma heparin concentration and the results of five heparin assays

Putten¹,

Ruit²,

Beunis³

et al. 1982

Clinica Chimica Acta

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“…Our earlier paper (2) as well as the modification described by Israels (3) present evidence that during heparin therapy a reliable assessment of the status of the intrinsic pathway can easily be obtained with a simple method consisting of a standard APTT plus an APTT with Polybrene addition. The latter part provides information on the intrinsic system without heparin interference and the ratio of both tests is a measure of the anticoagulant effect of heparin (2,3). The neutralization capacity of Polybrene is about 0.9 U of heparin per ml plasma, a value comparable to that of the platelet extract of Griffiths et al (1).…”

mentioning

confidence: 99%

“…In the discussion of this paper it is stated that information on the intrinsic pathway of coagulation is seldom, if ever available during a course of heparin therapy, because of technical difficulties. Evidently, the authors have overlooked at least four studies dealing with this very subject (2)(3)(4)(5). Besides, none of the techniques described in these studies is technically difficult, although some (2,3) are more practical to perform in the routine laboratory than others (4, 5).…”

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confidence: 99%

Monitoring the Intrinsic Pathway of Coagulation During Heparin Therapy

Hoffmann

1985

Thromb Haemost

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“…The activity of heparin can be estimated by co agulation tests [1,2] or by amidolytic chromogenic substrate assays [3], Clotting assays may show a large interindividual difference in heparin response [4,5] and a large depen dency on the reagents used [6,7], The hepa rin concentration accurately measured by a chromogenic substrate assay is assumed to represent the heparin concentration in vivo. It is well known that the results of clotting assays could be influenced by activation of clotting factors and by neutralization of hep arin during blood processing.…”

Section: Introductionmentioning

confidence: 99%

Heparin Neutralization during Collection and Processing of Blood Inhibited by Pyridoxal 5′-Phosphate

Putten¹,

Ruit²,

Beunis³

et al. 1984

Pathophysiol Haemos Thromb

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Spectrophotometric heparin assays are expected to be independent on clotting factors, either activated or nonactivated, but could be sensitive to heparin neutralization during blood collection. Is was shown that more than 200 U of heparin/1 plasma can completely be neutralized during blood processing. Because the heparin neutralization is not constant but dependent on the sample as well as on the type of sample handling, one cannot beforehand compensate and correct the results for possible heparin neutralization. We tested the use of pyridoxal 5’-phosphate (PLP) to prevent heparin neutralization. As the use of PLP in the citrate tube decreased the heparin neutralization to a negligible effect, PLP-citrate tubes are to be preferred for all plasma heparin determinations.

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Plasma heparin: A simple, one-sample determination of both effect and concentration based on the activated partial thromboplastin time

Cited by 10 publications

References 10 publications

Interindividual variation in relationships between plasma heparin concentration and the results of five heparin assays

Interindividual variation in relationships between plasma heparin concentration and the results of five heparin assays

Monitoring the Intrinsic Pathway of Coagulation During Heparin Therapy

Heparin Neutralization during Collection and Processing of Blood Inhibited by Pyridoxal 5′-Phosphate

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