Plasma inactivation of Aspergillus flavus on hazelnut surface in a diffuse barrier discharge using different working gases

Mošovská, Silvia; Medvecká, Veronika; Gregová, Mária; Tomeková, Juliána; Valı́k, Lubomı́r; Mikulajová, Anna; Zahoranová, Anna

doi:10.1016/j.foodcont.2019.05.003

Cited by 34 publications

(15 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The plasma treatment of barley samples was realized by a special type of atmospheric pressure discharge called diffuse coplanar surface barrier discharge (DCSBD) [ 62 ]. Unique design and optimized geometry allow the generation of low-temperature macroscopically uniform diffuse plasma in any working gas, even in electronegative, e.g., oxygen [ 63 ]. DCSBD consists of an alumina ceramic plate with two systems of parallel strip-like electrodes on the bottom side with alternating polarity of adjacent strips.…”

Section: Methodsmentioning

confidence: 99%

The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley

Petkova

Švubová

Kyzek

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Climate change, environmental pollution and pathogen resistance to available chemical agents are part of the problems that the food industry has to face in order to ensure healthy food for people and livestock. One of the promising solutions to these problems is the use of cold atmospheric pressure plasma (CAPP). Plasma is suitable for efficient surface decontamination of seeds and food products, germination enhancement and obtaining higher yields in agricultural production. However, the plasma effects vary due to plasma source, treatment conditions and seed type. In our study, we tried to find the proper conditions for treatment of barley grains by diffuse coplanar surface barrier discharge, in which positive effects of CAPP, such as enhanced germination or decontamination effects, would be maximized and harmful effects, such as oxidation and genotoxic potential, minimized. Besides germination parameters, we evaluated DNA damage and activities of various germination and antioxidant enzymes in barley seedlings. Plasma exposure resulted in changes in germination parameters and enzyme activities. Longer exposures had also genotoxic effects. As such, our findings indicate that appropriate plasma exposure conditions need to be carefully optimized in order to preserve germination, oxidation balance and genome stability, should CAPP be used in agricultural practice.

show abstract

Section: Methodsmentioning

confidence: 99%

The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley

Petkova

Švubová

Kyzek

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Dasan et al [12] also observed the 4 log reduction of A. flavus after 5 min of plasma treatment on hazelnut samples. Mošovská et al [21] studied the cold plasma using different gases (O2, N2, ambient air, synthetic air with or without vapor) on hazelnut samples and observed that the use of synthetic air (with vapor) as plasma working gas reduced the A. flavus spore population below the detection limit (4 log) after 180 s of the treatment. Sen et al [4] also reported that population of A. flavus and A. parasiticus in hazelnut samples decreased by about 4 log after low pressure (LP) plasma treatment (100 W for 30 min).…”

Section: Decontamination Effect Of Plasma Treatmentmentioning

confidence: 99%

Elimination of Aspergillus flavus from Pistachio Nuts with Dielectric Barrier Discharge (DBD) Cold Plasma and Its Impacts on Biochemical Indices

Makari¹,

Hojjati²,

Shahbazi

et al. 2021

Journal of Food Quality

View full text Add to dashboard Cite

In the present research, the effects of different durations (0, 15, 30, 60, 90, 120, 150, and 180 sec) of dielectric barrier discharge (DBD) cold plasma on decontaminating Aspergillus flavus, detoxifying pure aflatoxin B1 (AFB1), and the quality attributes of pistachio nuts (total phenolic content, antioxidant activity, chlorophylls, total carotenoids, instrumental color, total soluble protein, and malondialdehyde determination) were studied. The results showed that the viable spore population reduced with the increase of plasma treatment duration, so that after 180 s of the treatment, a decrease by 4 logs was observed in the spore population. Chlorophyll a and b, as well as total carotenoid levels and color parameters, decreased, which led to darker pistachio samples and intensity reduction in soluble protein content and protein bands. Plasma treatment did not alter the total phenolic content but slightly increased the antioxidant activity of pistachio nuts samples. The malondialdehyde values significantly increased all the plasma treatment durations. The maximum reduction of AFB1 was observed after 180 s of the treatment, which was 64.63% and 52.42% for glass slides and pistachio nut samples, respectively. The present findings demonstrated that cold plasma could be used as an efficient decontamination method of food products without inducing undesirable quality changes in nuts.

show abstract

“…In the oxygen atmosphere, only the presence of ozone was recorded [ 6 ]. Treatment of seed surfaces by non-thermal plasma generated in different gases effectively reduces the amount of pathogenic microorganism, which colonize corn grains [ 7 ], nuts [ 8 , 9 ], barley and wheat grains [ 1 ], and pine seeds [ 10 ]. Non-thermal plasma modifies seed surface, which markedly changes its affinity to water, and this is very important for successful imbibition and starting of germination processes.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Impact of Cold Atmospheric Pressure Plasma on Soybean Seed Germination

Švubová

Slováková

Holubová

et al. 2021

Plants

Self Cite

View full text Add to dashboard Cite

The present study aims to define the effects of Cold Atmospheric Pressure Plasma (CAPP) exposure on seed germination of an agriculturally important crop, soybean. Seed treatment with lower doses of CAPP generated in ambient air and oxygen significantly increased the activity of succinate dehydrogenase (Krebs cycle enzyme), proving the switching of the germinating seed metabolism from anoxygenic to oxygenic. In these treatments, a positive effect on seed germination was documented (the percentage of germination increased by almost 20% compared to the untreated control), while the seed and seedling vigour was also positively affected. On the other hand, higher exposure times of CAPP generated in a nitrogen atmosphere significantly inhibited succinate dehydrogenase activity, but stimulated lactate and alcohol dehydrogenase activities, suggesting anoxygenic metabolism. It was also found that plasma exposure caused a slight increment in the level of primary DNA damage in ambient air- and oxygen-CAPP treatments, and more significant DNA damage was found in nitrogen-CAPP treatments. Although a higher level of DNA damage was also detected in the negative control (untreated seeds), this might be associated with the age of seeds followed by their lower germination capacity (with the germination percentage reaching only about 60%).

show abstract

Plasma inactivation of Aspergillus flavus on hazelnut surface in a diffuse barrier discharge using different working gases

Cited by 34 publications

References 22 publications

The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley

The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley

Elimination of Aspergillus flavus from Pistachio Nuts with Dielectric Barrier Discharge (DBD) Cold Plasma and Its Impacts on Biochemical Indices

Evaluation of the Impact of Cold Atmospheric Pressure Plasma on Soybean Seed Germination

Contact Info

Product

Resources

About