Plasma induced patterning of polydimethylsiloxane surfaces

Hsieh, Shou-Shing; Cheng, Yue-an; Hsieh, Chiung-Wen; Liu, YiLen

doi:10.1016/j.mseb.2008.10.036

Cited by 14 publications

(15 citation statements)

References 38 publications

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“…Within the framework of micro/nanotechnologies, polymer-based materials such as polymethylmethacrylate (PMMA) [1,2], poly-vinyl chloride (PVC) [3][4][5], poly-vinyl alcohol (PVA) [6,7], polysiloxane (PSX) [8], SU-8 epoxy resin [9][10][11][12], polydimethylsiloxane (PDMS) [13][14][15][16][17], benzocyclobutene (BCB) [18][19][20][21][22],…, have shown very promising potentialities for a wide range of applications: sacrificial layers techniques, surface micropatterning, micro/nanomaterials integration, dielectric insulation, packaging (from wafer to system level),… Nevertheless, whatever the polymer chemistry, the mass-fabrication requirement has been carried out through the development of specific micro/nanolithography processes based on inkjet printing, spray coating, micro/nano contact printing, micromoulding, serigraphy shadow mask patterning and of course photolithography. This last process is well adapted to wafer level packaging techniques.…”

Section: Introductionmentioning

confidence: 99%

Theoretical studies of the spin coating process for the deposition of polymer-based Maxwellian liquids

Temple‐Boyer

Mazenq

Doucet

et al. 2010

Microelectronic Engineering

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This paper deals with the study of spin coating processes for the deposition of polysiloxane-based thin films. Specific developments are proposed in order to adapt the hydrodynamic laws theory to the spin coating of Maxwellian liquids. Theoretical and experimental results are compared, evidencing a good fit and enabling to define the Maxwellian law for the studied polysiloxane polymer. Finally, the theoretical developments are successfully extended to the BCB 4026 and SU-8 3050 photosensitive resins.

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Section: Introductionmentioning

confidence: 99%

Theoretical studies of the spin coating process for the deposition of polymer-based Maxwellian liquids

Temple‐Boyer

Mazenq

Doucet

et al. 2010

Microelectronic Engineering

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“…However, the introduction of these adhesion promoters may affect cell migration through chemical interactions, thus influencing the migration behavior. As discussed above, plasma treatment is commonly used to oxidize hydrophobic polymer surfaces, transforming the surface free energy to a hydrophilic state [24,26]. Our plasma-based method allows us to further control the chemical pattern (size and shape) through selective oxidation of hydrophobic PDMS to create samples for cell patterning that do not require substrate adhesion molecules.…”

Section: Resultsmentioning

confidence: 99%

“…A copper TEM grid (400 mesh EMITECH -square type -3.2 mm diameter) was placed on the cured PDMS surfaces and then the samples were exposed to a lowpressure "air" plasma (53.3 Pa, 2 min, 10.5 W -Harrick Scientific Products, Inc. Model PDC-001) to selectively generate oxidation patterns on the hydrophobic PDMS surfaces. Details of the substrate preparation and micropatterning method are described elsewhere [26]. supplemented with 10% fetal bovine serum (FBS) (Gibco, Invitrogen, Carlsbad, California) at 37 ºC in a humidified atmosphere of 5% CO 2 .…”

Section: Substrate Preparation and Micropatterningmentioning

confidence: 99%

“…This is closely related to the interaction between cells and the patterned PDMS substrate. Plasma patterning selectively transformed the PDMS surface from a hydrophobic (surface stoichiometry: n-Si-(CH 3 ) 2 -O-n) and cell repellent state, to a hydrophilic (SiO 2 ) and roughened state, which promoted cell adhesion and growth [24,26]. However, molecules presented at the apical membranes of HCC cells may be one of key roles in influencing the strength of cell adhesion.…”

Section: Cell Activity Is Controlled By Chemical and Mechanical Intermentioning

confidence: 99%

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Hepatocarcinoma Single Cell Migration on Micropatterned PDMS Substrates

Zheng¹,

Hsieh

Wu³

et al. 2011

Nano BioMed ENG

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Cell migration influences many normal and pathological processes and is one of key issues addressed in cancer research studies. In this report, a plasma patterned polydimethylsiloxane (PDMS) substrate was used to selectively position hepatocarcinoma cells in order to characterize their migration behavior. We observed that cell mobility was directly related to the differentiation stage of the cells, with poorly-differentiated (SK-Hep-1) cells exhibiting higher mobility that well-differentiated (Hep-G2) cells. We propose that this difference occurs due to a loss of adhesion molecules presented at the apical membranes of the poorly-differentiated SK-Hep-1 cells, thereby reducing their adhesion to the surface. Our results provide new insight into the relationship between carcinoma cell differentiation grade and mobility. Further this experimental process may provide a simple and effective model for universal cell biology studies and applications in microsystems technology.

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“…All cantilever probes were plasma cleaned [14] (Harrick Scientific Products, Inc.) for 2 min using dry air as the reactive gas to increase the OH concentration at the surface [15, 16]. This process creates a uniformly hydroxylated surface.…”

Section: Methodsmentioning

confidence: 99%

Label-Free Glucose Detection Using Cantilever Sensor Technology Based on Gravimetric Detection Principles

Hsieh

Hsieh²,

Lin³

et al. 2013

Journal of Analytical Methods in Chemistry

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Efficient maintenance of glucose homeostasis is a major challenge in diabetes therapy, where accurate and reliable glucose level detection is required. Though several methods are currently used, these suffer from impaired response and often unpredictable drift, making them unsuitable for long-term therapeutic practice. In this study, we demonstrate a method that uses a functionalized atomic force microscope (AFM) cantilever as the sensor for reliable glucose detection with sufficient sensitivity and selectivity for clinical use. We first modified the AFM tip with aminopropylsilatrane (APS) and then adsorbed glucose-specific lectin concanavalin A (Con A) onto the surface. The Con A/APS-modified probes were then used to detect glucose by monitoring shifts in the cantilever resonance frequency. To confirm the molecule-specific interaction, AFM topographical images were acquired of identically treated silicon substrates which indicated a specific attachment for glucose-Con A and not for galactose-Con A. These results demonstrate that by monitoring the frequency shift of the AFM cantilever, this sensing system can detect the interaction between Con A and glucose, one of the biomolecule recognition processes, and may assist in the detection and mass quantification of glucose for clinical applications with very high sensitivity.

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Plasma induced patterning of polydimethylsiloxane surfaces

Cited by 14 publications

References 38 publications

Theoretical studies of the spin coating process for the deposition of polymer-based Maxwellian liquids

Theoretical studies of the spin coating process for the deposition of polymer-based Maxwellian liquids

Hepatocarcinoma Single Cell Migration on Micropatterned PDMS Substrates

Label-Free Glucose Detection Using Cantilever Sensor Technology Based on Gravimetric Detection Principles

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