Plasma induced reactive oxygen species‐dependent cytotoxicity in glioblastoma 3D tumourspheres

Wanigasekara, Janith; Barcia, Carlos; Cullen, Patrick J.; Tiwari, Brijesh K.; Curtin, James F.

doi:10.1002/ppap.202100157

Cited by 17 publications

(46 citation statements)

References 37 publications

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“…The fourth state of matter, known as plasma, can be seen as an arc or discharge of intense fluorescent light [7]. A mixture of reactive species including electrons, photons, positively or negatively charged ions, free radicals, and atoms in their excited and ground states make up plasma, which is a partially or entirely ionised gas [8]. Plasma has a net neutral charge and is referred to as quasineutral.…”

Section: Cp Technology In the Food Industrymentioning

confidence: 99%

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Cold Plasma Technology in Food Packaging

et al. 2022

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Cold plasma (CP) is an effective strategy to alter the limitations of biopolymer materials for food packaging applications. Biopolymers such as polysaccharides and proteins are known to be sustainable materials with excellent film-forming properties. Bio-based films can be used as an alternative to traditional plastic packaging. There are limitations to biopolymer packaging materials such as hydrophobicity, poor barrier, and thermos-mechanical properties. For this reason, biopolymers must be modified to create a packaging material with the desired applicability. CP is an effective method to enhance the functionality and interfacial features of biopolymers. It etches the film surface allowing for better adhesion between various polymer layers while also improving ink printability. CP facilitates adhesion between two or more hydrophobic materials, resulting in significantly better water vapour permeability (WVP) properties. The sputtering of ionic species by CP results in cross-linkage reactions which improve the mechanical properties of films (tensile strength (TS) and elongation at break (EAB)). Cross-linkage reactions are reported to be responsible for the improved thermal stability of CP-treated biopolymers. CP treatment is known to decrease oxygen permeability (OP) in protein-based biopolymers. CP can also enable the blending of polymers with specific antimicrobial substances to develop active packaging materials. In this review article, we have presented an overview of the recent advancements of CP in the food packaging application. Furthermore, the influence of CP on the properties of packaging materials, and recent advancements in the modification of polymeric food packaging materials have been discussed.

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Section: Cp Technology In the Food Industrymentioning

confidence: 99%

“…The generation of thermal plasma requires a high pressure above 105 Pa and an electrical supply greater than 50 MV. Non-thermal plasma can exist without a localised thermodynamic equilibrium and does not require high pressure or power [8].…”

Section: Cp Technology In the Food Industrymentioning

confidence: 99%

Cold Plasma Technology in Food Packaging

et al. 2022

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“…The main benefits of employing 3D cell culture for drug discovery include greater cell-cell contact, ECM-cell interactions, varied rates of cellular proliferation, oxygen and nutrition availability, physiological gradients for nutrients, waste, signalling factors and drugs, and the additional influence of stroma, all of which assist in replicating natural tissue distribution [ 1 , 5 , 10 ]. It can also simulate drug resistance, cellular microenvironment activity, and the expression of intrinsic and genetic variables [ 7 , 10 ]. The declared goal of the European REACH legislation is “To ensure a high level of protection of human health and the environment from the effects of hazardous chemicals.…”

Section: Introductionmentioning

confidence: 99%

“…It strives for a balance: to increase our understanding of the possible hazards of chemicals, while at the same time avoiding unnecessary testing on animals” (European Chemicals Agency, 2020). 3D cell cultures complement the 3Rs principles of animal research (Replacement, Reduction, and Refinement) and REACH regulations by reducing the number of animals used in testing, as well as saving time, money, and ethical issues [ 1 , 10 ]. Animal testing is both costly and time consuming.…”

Section: Introductionmentioning

confidence: 99%

“…Animal testing is both costly and time consuming. Furthermore, if the animal is in pain or under stress throughout the experiment, it may alter the biochemical, physiological, and metabolic processes, which might misrepresent the efficacy and adverse effects of drugs [ 6 , 9 , 10 ]. 3D spheroid cultures can be readily established in many cell culture facilities using anchorage-independent or anchorage-dependent methods.…”

Section: Introductionmentioning

confidence: 99%

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Three-Dimensional (3D) in vitro cell culture protocols to enhance glioblastoma research

et al. 2023

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Three-dimensional (3D) cell culture models can help bridge the gap between in vitro cell cultures and in vivo responses by more accurately simulating the natural in vivo environment, shape, tissue stiffness, stressors, gradients and cellular response while avoiding the costs and ethical concerns associated with animal models. The inclusion of the third dimension in 3D cell culture influences the spatial organization of cell surface receptors that interact with other cells and imposes physical restrictions on cells in compared to Two-dimensional (2D) cell cultures. Spheroids’ distinctive cyto-architecture mimics in vivo cellular structure, gene expression, metabolism, proliferation, oxygenation, nutrition absorption, waste excretion, and drug uptake while preserving cell–extracellular matrix (ECM) connections and communication, hence influencing molecular processes and cellular phenotypes. This protocol describes the in vitro generation of tumourspheroids using the low attachment plate, hanging drop plate, and cellusponge natural scaffold based methods. The expected results from these protocols confirmed the ability of all these methods to create uniform tumourspheres.

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Synergistic cytotoxicity effect of ultrasound and plasma microbubble in glioblastoma 3D tumor sphere model

Wanigasekara,

Cullen,

Tiwari

et al. 2024

Plasma Processes & Polymers

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The combined application of ultrasound (US) and plasma microbubble (PMB) is a promising technology against glioblastoma (GBM). Here, we determined the effects of US and PMB (alone and combined) on U‐251 MG GBM and A431 epidermoid carcinoma spheroids under different conditions. The synergistic treatment of US‐ and PMB‐induced tumor sphere cell death in a time‐, dose‐, treatment frequency‐, and reactive oxygen species dependent manner, while multiple treatments augmented this effect. Synergistic treatments significantly reduced three‐dimensional GBM spheroid regrowth and cell proliferation while inducing cytotoxicity, spheroid shrinkage, and damage to the tumor microenvironment. PMB‐induced cytotoxicity throughout the tumor sphere, likely via long‐lived reactive oxygen and nitrogen species (H2O2, NO2−, and NO3−) and other reactive species.

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Plasma induced reactive oxygen species‐dependent cytotoxicity in glioblastoma 3D tumourspheres

Cited by 17 publications

References 37 publications

Cold Plasma Technology in Food Packaging

Cold Plasma Technology in Food Packaging

Three-Dimensional (3D) in vitro cell culture protocols to enhance glioblastoma research

Synergistic cytotoxicity effect of ultrasound and plasma microbubble in glioblastoma 3D tumor sphere model

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