Plasma iron levels appraised 15 days after spinal cord injury in a limb movement animal model

Reis, Fabiana Medeiros D.; Esteves, A M; Tufik, Sérgio; Mello, Marco Túlio de

doi:10.1038/sc.2010.121

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…The candidate protein selection was based on the spinal cord specificity, antibody availability, and levels of peptide abundance. Five biomarker candidates were selected, blood brain barrier (BBB) located transferrin (49), lysosomal protease cathepsin D (50), triosephosphate isomerase-1 (TIM, TPI-1) (16), astrocytic phosphoprotein of 15 kDa (PEA-15) (51), and potential stroke biomarker nucleoside diphosphate kinase A (52) to confirm their protein alterations associated with SCI. The proteolytic damage markers to the spinal cord structural and cellular components have been validated by quantitative immunoblotting using specific antibodies for each protein.…”

Section: Table II Identification Of Differentially Expressed Proteinsmentioning

confidence: 99%

Differential Neuroproteomic and Systems Biology Analysis of Spinal Cord Injury

Moghieb

Bramlett

Das

et al. 2016

Molecular & Cellular Proteomics

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Acute spinal cord injury (SCI) is a devastating condition with many consequences and no known effective treatment. Although it is quite easy to diagnose traumatic SCI, the assessment of injury severity and projection of disease progression or recovery are often challenging, as no consensus biomarkers have been clearly identified. Here rats were subjected to experimental moderate or severe thoracic SCI. At 24h and 7d postinjury, spinal cord segment caudal to injury center versus sham samples was harvested and subjected to differential proteomic analysis. Cationic/anionic-exchange chromatography, followed by 1D polyacrylamide gel electrophoresis, was used to reduce protein complexity. A reverse phase liquid chromatography-tandem mass spectrometry proteomic platform was then utilized to identify proteome changes associated with SCI. Twenty-two and 22 proteins were up-regulated at 24 h and 7 day after SCI, respectively; whereas 19 and 16 proteins are down-regulated at 24 h and 7 day after SCI, respectively, when compared with sham control. A subset of 12 proteins were identified as candidate SCI biomarkers -TF (

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Section: Table II Identification Of Differentially Expressed Proteinsmentioning

confidence: 99%