Plasma levels of chemokine ligand 20 and chemokine receptor 6 in patients with sepsis

Klaus, Daniel A.; Seemann, Rudolf; Roth‐Walter, Franziska; Einwallner, Elisa; Motal, Michael C.; Tudor, Bianca; Lebherz-Eichinger, Diana; Wiegele, Marion; Krenn, Claus G.

doi:10.1097/eja.0000000000000388

Cited by 6 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…24,25 Several animal studies have confirmed that MIP-3 alpha is involved in the progression of severe inflammation, [26][27][28] and a recent study demonstrated that the MIP-3 alpha-driven cascade promoted the development of systemic inflammation in patients with severe sepsis. 29 They demonstrated plasma levels of MIP-3alpha in ICU patients with sepsis were elevated. Consistent with previous research, this study revealed that the concentration of MIP-3alpha in the death group was significantly higher than that in the survival group.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Value of Macrophage Inflammatory Protein-3alpha (MIP3-Alpha) and Severity Scores in Elderly Patients with Sepsis

Liu,

Duan,

Zhang

et al. 2024

JIR

View full text Add to dashboard Cite

Objective This study examines the effectiveness of MIP-3alpha and severity scores in determining the prognosis of elderly sepsis patients. Methods From October 2020 to April 2021, a total of 171 elderly sepsis patients were admitted to the Emergency Department of the Shijingshan Branch of Beijing Chaoyang Hospital, Capital Medical University. According to the 28-day mortality rate, they were divided into two groups: survivors (48 cases) and deaths (123 cases). At admission, severity scores which are the Sequential Organ Failure Assessment (SOFA) and the Acute Physiology and Chronic Health Evaluation II (APACHE II) were calculated. The logistic regression was used to analyze the independent risk factors associated with 28-day mortality in elderly sepsis patients. The receiver operating characteristic (ROC) curve and area under the curve (AUC) were used to evaluate the value of MIP-3alpha, SOFA, and APACHE II in the evaluation of 28-day mortality in elderly sepsis patients. Results MIP-3alpha, SOFA and APACHE II of the death group were significantly higher than those of the survival group (P < 0.05). Multivariate logistic regression analysis showed that MIP-3alpha, SOFA, APACHE II, and systolic blood pressure (SBP) were independent risk factors for 28-day mortality of senile sepsis (P < 0.05). Analysis of the ROC curve revealed that MIP-3alpha, SOFA, APACHE II had predictive value for the 28-day prognosis of senile sepsis (all P < 0.01). Combing with MIP-3alpha and SOFA showed better predictive ability (Z1 = 3.733, Z2 = 2.996, both P < 0.01), compared to detecting MIP-3alpha and SOFA alone. Conclusion In senile sepsis, MIP-3alpha, SOFA, APACHE II and SBP are independent risk factors for 28-day mortality. The combination of MIP-3alpha and SOFA can further enhance the predictive value of 28-day mortality in patients with senile sepsis and provide some reference value for the evaluation and treatment of senile sepsis.

show abstract

Section: Discussionmentioning

confidence: 99%

Prognostic Value of Macrophage Inflammatory Protein-3alpha (MIP3-Alpha) and Severity Scores in Elderly Patients with Sepsis

Liu,

Duan,

Zhang

et al. 2024

JIR

View full text Add to dashboard Cite

show abstract

“…In sepsis patients, the population of B-PG#1 (CCR6 high CCR7 low HLA-DR low subset) was increased, while that of B-PG#2 (CCR6 low CCR7 mid HLA-DR high subset) was decreased. Since CCR6 is a crucial component in B cell immune responses and is associated with serious organ failure, 32 the elevated CCR6 in the B-PG#1 subset implies its role in sepsis-driven B cell activation. Furthermore, as the elevation of CCR7 expression is closely related to the differentiation of pre-B cells to the immature and mature stages, 33 the low CCR7 expression in B-PG#1 indicated a malfunction in B cell differentiation.…”

Section: ■ Discussionmentioning

confidence: 99%

Phenotypic Landscape of Immune Cells in Sepsis: Insights from High-Dimensional Mass Cytometry

Park,

Perumalsamy,

Gerelkhuu

et al. 2024

ACS Infect. Dis.

View full text Add to dashboard Cite

Understanding the sepsis-induced immunological response can be facilitated by identifying phenotypic changes in immune cells at the single-cell level. Mass cytometry, a novel multiparametric single-cell analysis technique, offers considerable benefits in characterizing sepsis-induced phenotypic changes in peripheral blood mononuclear cells. Here, we analyzed peripheral blood mononuclear cells from 20 sepsis patients and 10 healthy donors using mass cytometry and employing 23 markers. Both manual gating and automated clustering approaches (PhenoGraph) were used for cell identification, complemented by uniform manifold approximation and projection (UMAP) for dimensionality reduction and visualization. Our study revealed that patients with sepsis exhibited a unique immune cell profile, marked by an increased presence of monocytes, B cells, and dendritic cells, alongside a reduction in natural killer (NK) cells and CD4/CD8 T cells. Notably, significant changes in the distributions of monocytes and B and CD4 T cells were observed. Clustering with PhenoGraph unveiled the subsets of each cell type and identified elevated CCR6 expression in sepsis patients' monocyte subset (PG#5), while further PhenoGraph clustering on manually gated T and B cells discovered sepsis-specific CD4 T cell subsets (CCR4 low CD20 low CD38 low ) and B cell subsets (HLA-DR low CCR7 low CCR6 high ), which could potentially serve as novel diagnostic markers for sepsis.

show abstract

Outlining key inflammation‐associated parameters during early phase of an experimental gram‐negative sepsis model in rhesus macaques (Macaca mulatta)

Rosado‐Franco

Ramos‐Benitez

Parodi

et al. 2019

Anim Models and Exp Med

View full text Add to dashboard Cite

The aim of this study was to identify inflammation‐associated markers during the early phase of sepsis in rhesus macaque. Four rhesus macaques were given an intravenous dose of 1010 CFU/kg of E. coli. Blood samples were collected before, or 30 minutes, 2, 4, 6 and 8 hours after E. coli infusion. Physiological parameters, bacteremia, endotoxemia, C‐reactive protein (CRP), procalcitonin (PCT), and plasma cytokines/chemokines were determined for each animal. Bacteremia was present in all animals from 30 minutes to 3 hours after E. coli infusion whereas endotoxin was detected during the full‐time course. CRP and PCT levels remained at detectable levels during the whole experimental window suggesting an ongoing inflammatory process. Signature cytokines and chemokines such as TNF‐α, MIP‐1α, and MIP‐1β peaked about 2 hours after E. coli infusion and decreased thereafter. Plasma IL‐6, IL‐12p40, IFN‐γ, and IL‐1Ra, as well as I‐TAC, MIG, IP‐10 and MCP‐1, remained at detectable levels after 4 hours of E. coli infusion. This nonhuman primate model could be useful for the assessment of new therapeutics aiming to suppress key inflammatory markers throughout sepsis early phases.

show abstract

Plasma levels of chemokine ligand 20 and chemokine receptor 6 in patients with sepsis

Abstract: There were early increased plasma concentrations of CCL20 and CCR6 in patients with sepsis. CCL20 and CCR6 correlate with severity of illness in ICU patients. Levels of CCR6 predicted the length of patients' admission.

Cited by 6 publications

References 28 publications

Prognostic Value of Macrophage Inflammatory Protein-3alpha (MIP3-Alpha) and Severity Scores in Elderly Patients with Sepsis

Prognostic Value of Macrophage Inflammatory Protein-3alpha (MIP3-Alpha) and Severity Scores in Elderly Patients with Sepsis

Phenotypic Landscape of Immune Cells in Sepsis: Insights from High-Dimensional Mass Cytometry

Outlining key inflammation‐associated parameters during early phase of an experimental gram‐negative sepsis model in rhesus macaques (Macaca mulatta)

Contact Info

Product

Resources

About