Plasma Levels of Gonadal Steroids during Final Oocyte Maturation of Striped Bass, Morone saxatilis L.

King, William; Thomas, Peter; Harrell, Reginal M.; Hodson, Ronald G.; Sullivan, Craig V.

doi:10.1006/gcen.1994.1115

Cited by 91 publications

(42 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding corroborates a previous report on elevated levels of these two hormones in striped bass during natural and pharmacologicallyinduced FOM (King et al 1994a). In that study, approximately half of the 203-S immunoreactivity coeluted with a 5B1,3a-hydroxylated form of 201-S identified by reverse-phase HPLC of extracted plasma.…”

Section: Effects Of Steroids and Inhibitors On Fom In Vitrosupporting

confidence: 81%

“…Previous studies of striped bass (Morone saxatilis), a species that recruits and spawns a single batch of eggs annually, showed that plasma DHP and 2013-S levels increased as levels of E 2 and T decreased in fish undergoing FOM (Berlinsky and Specker 1991; King et al 1994a). Similarly, both DHP and 2013-S were produced by striped bass ovarian fragments undergoing hCG-induced GVBD in vitro, while production of E 2 and T declined (King et al 1994b).…”

Section: Introductionmentioning

confidence: 91%

“…Biopsy samples were chemically-cleared in fixative (ethanol:formalin: acetic acid; 6:3:1, v/v) to determine the position of the oocyte germinal vesicle (GV) at the beginning of the experiment (King et al 1994a). Animals were identified with colored streamer tags (Floy Manufacturing; Seattle, WA) and injected with hCG (330 IU kg-l body weight i.m.).…”

Section: Induction Of Final Oocyte Maturation In Vivomentioning

confidence: 99%

“…Residues were resuspended in 200 tl of RIA buffer and measured by RIA as previously described for E 2 and T (Woods and Sullivan 1993) or for DHP and 201-S (King et al 1994a). The steroid RIA's were validated for use with both white bass and white perch plasma and culture media (King et al 1994b).…”

Section: Radioimmunoassaymentioning

confidence: 99%

See 3 more Smart Citations

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

King

Berlinsky

Sullivan

1995

Fish Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Plasma estradiol-17β (E2), testosterone (T), 17α,20β-dihydroxy-4-pregnen-3-one (DHP) and 17α,20β,21-tri-hydroxy-4-pregnen-3-one (20β-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20β-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E2 and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20β-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E2 and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20β-S and oocyte GVBD by white perch follices. DHP and 20β-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20β-S in the control of FOM in white perch and white bass.

show abstract

Section: Effects Of Steroids and Inhibitors On Fom In Vitrosupporting

confidence: 81%

Section: Introductionmentioning

confidence: 91%

Section: Induction Of Final Oocyte Maturation In Vivomentioning

confidence: 99%

Section: Radioimmunoassaymentioning

confidence: 99%

See 2 more Smart Citations

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

King

Berlinsky

Sullivan

1995

Fish Physiol Biochem

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, cAMP levels in both receptortransfected cell lines were significantly decreased in a concentration-and time-dependent manner in response to physiologically relevant concentrations of 17,20b-DHP. Although circulating levels of 17,20b-DHP have not yet been measured in zebrafish due to their small size, MIS concentrations (1-10 nM) that were effective in significantly decreasing cAMP levels and activating MAPK in this experiment are within the range found in the plasma of a variety of fish species during oocyte maturation and ovulation (Kobayashi et al 1987, Truscott et al 1992, King et al 1994. The finding that the MIS, 17,20b-DHP, is the preferred ligand for the zebrafish mPRs is important for establishing their ovarian functions in the presence of a variety of similar progestin derivatives in the ovary.…”

Section: Discussionmentioning

confidence: 69%

Cell-surface expression, progestin binding, and rapid nongenomic signaling of zebrafish membrane progestin receptors α and β in transfected cells

Hanna¹,

Pang²,

Thomas³

et al. 2006

Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

Recently, a unique family of membrane progestin receptors (mPRa, mPRb, and mPRg) was identified, which may be responsible for mediating rapid, nongenomic actions of progestins in a variety of target tissues. In this study, the mPRa and mPRb isoforms from zebrafish were shown to be rapidly and specifically activated by the maturation-inducing steroid (MIS) of this species, . The zebrafish mPRa and a previously uncharacterized mPRb isoform were stably expressed in nuclear progesterone receptor-deficient mammalian breast cancer cells, MDA-MB-231. Expression and surface localization of the receptors were verified by flow cytometry, biotin surface labeling, and Western blotting. Plasma membrane proteins from mPRa-or mPRb-transfected cells showed high affinity (mPRa, K d 7 nM; mPRb, K d 12 nM), saturable, displaceable, single-binding sites specific for 17,20b-DHP, whereas negligible specific 17,20b-DHP binding was observed in nontransfected cells. Progestin treatment caused significant activation of mitogen-activated protein kinase (MAPK) within 5 min in cells transfected with either of the receptors as measured by western blotting and flow cytometry. The rank order of the potencies of several progestins in activating MAPK via mPRa and mPRb was the same (17,20b,. Interestingly, the MIS in zebrafish, 17,20b-DHP, was also the most potent inhibitor, among the progestins tested, of adenylyl cyclase activity in cells transfected with either of the receptors. This progestin significantly decreased cAMP levels in both mPRa-and mPRb-transfected cells in a dose-responsive and timedependent manner. In addition, signaling of the zebrafish mPRa was blocked by pertussis toxin, implying activation of a G i protein, while sensitivity to pertussis or cholera toxin was not shown with mPRb-mediated signaling, possibly indicating that this receptor activates a different pertussis toxin-insensitive G protein. The results of this study suggest that zebrafish mPRa and mPRb signal similarly upon progestin binding resulting in rapid activation of MAPK and downregulation of adenylyl cyclase activity.

show abstract

Characterization of the mRNA expression of StAR and steroidogenic enzymes in zebrafish ovarian follicles

Ings

Kraak

2006

Molecular Reproduction Devel

110

View full text Add to dashboard Cite

The objective of this study was to investigate the levels of expression of steroid biosynthetic enzymes and steroidogenic acute regulatory protein (StAR) at different stages of ovarian follicular development in zebrafish (Danio rerio), and to investigate the sites within the steroid biosynthetic pathway that may be regulated by gonadotropins. Ovarian follicles of sexually mature fish were separated into primary, previtellogenic, vitellogenic, and mature stages and the expression of StAR, P450 side chain cleavage (P450scc), 3beta-hydroxysteroid dehydrogenase (3beta-HSD), P450 hydroxylase/lyase (P450c17), 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1), 17beta-hydroxysteroid dehydrogenase type 3 (17beta-HSD3), and P450 aromatase (P450aromA) was determined by Real time RT-PCR. The expression of all genes changed significantly as follicles grew, with a decrease in the expression of StAR, P450scc, 3beta-HSD and P450c17 with maturation, and an increase in the expression of 17beta-HSD3 during vitellogenesis and 17beta-HSD1 and P450aromA during previtellogenesis. In vitro incubation of vitellogenic follicles demonstrated that the expression of StAR, 17beta-HSD3, and P450aromA increased in response to hCG, and decreased in the absence of hCG. In contrast, the expression of P450scc, 3beta-HSD, P450c17, and 17beta-HSD1 remained constant between treatments and over time. Testosterone and estradiol production in the culture medium was stimulated by human chorionic gonadotropin (hCG). These experiments aid in the characterization of the roles and regulation of steroids throughout ovarian development, and suggest that gonadotropins play a key role in the regulation of StAR, 17beta-HSD3, and P450aromA in zebrafish.

show abstract

Plasma Levels of Gonadal Steroids during Final Oocyte Maturation of Striped Bass, Morone saxatilis L.

Cited by 91 publications

References 0 publications

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

Cell-surface expression, progestin binding, and rapid nongenomic signaling of zebrafish membrane progestin receptors α and β in transfected cells

Characterization of the mRNA expression of StAR and steroidogenic enzymes in zebrafish ovarian follicles

Contact Info

Product

Resources

About