Plasma levels of soluble cellular adhesion molecules in patients with arterial hypertension. Correlations with plasma endothelin-1

Parissis, John; Venetsanou, Koula; Mentzikof, D.; Kalantzi, Maria; Georgopoulou, Maria; Chrisopoulos, Nikolaos; Karas, S.

doi:10.1016/s0953-6205(01)00125-x

Cited by 40 publications

(32 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Andre et al[ 12] found that sP-selectin constitutes an endogenous activator of the coagulation process through the generation of circulating microparticles in plasma. The relationship between sP-selectin and hypercholesterolemia has also been confirmed by other authors [13, 14]. One of them observed a positive correlation between sP-selectin and von Willebrand factor in the hypercholesterolemic group [14].…”

Section: Discussionsupporting

confidence: 70%

The Influence of Hyperlipidemia on Platelet Activity Markers in Patients after Ischemic Stroke

Pawełczyk¹,

Baj²,

Chmielewski³

et al. 2008

Cerebrovasc Dis

View full text Add to dashboard Cite

Background: To investigate the relationship between hyperlipidemia and platelet activation markers – platelet and soluble P-selectin (sP-selectin), and platelet-derived microparticles (PDMPs) – in patients after ischemic stroke. Methods: 41 patients after ischemic stroke (>3 months) confirmed by CT were divided into 2 groups: with hyperlipidemia (HL, n = 21) and normolipidemia (NL, n = 20). Twenty healthy subjects served as controls. CD62P-positive platelets and PDMPs in whole blood were analyzed by the use of a flow cytometer and anti-CD61 and anti-CD62P monoclonal antibodies. Platelets were activated by thrombin (0.08 units). The level of sP-selectin in serum was measured by ELISA. Results: We observed a significantly higher CD62P expression and percentage of CD62P-positive resting and thrombin-activated platelets in the HL as compared to the NL group. The sP-selectin concentration was also significantly higher in HL than NL subjects (p < 0.05). Moreover, we observed a significantly higher percentage of PDMPs in patients after stroke (NL: p < 0.05; HL: p = 0.005) in comparison with the control group. Conclusions: Patients after stroke present symptoms of platelet hyperreactivity. HL in the patients may be a risk factor for vascular events due to the increase in platelet activation.

show abstract

Section: Discussionsupporting

confidence: 70%

The Influence of Hyperlipidemia on Platelet Activity Markers in Patients after Ischemic Stroke

Pawełczyk¹,

Baj²,

Chmielewski³

et al. 2008

Cerebrovasc Dis

View full text Add to dashboard Cite

show abstract

“…Moreover, the sVCAM-1 concentration is positively associated with vascular media thickness [39]. It has been reported that the circulating concentration of sVCAM-1 in hypertension patients (681 ± 42 ng/ml) is higher than that of normotensive subjects (589 ± 23 ng/ml) [14]. In this study, sVCAM-1 concentrations of 0.1 µg/ml or higher increased RASMC migration and proliferation.…”

Section: Discussionsupporting

confidence: 58%

“…sVCAM-1 in the blood of diabetic and cancer patients was found at a higher level than in normal subjects [13]. Moreover, the serum level of sVCAM-1 is increased in atherosclerosis and hypertension [1, 14, 15]. These results imply that sVCAM-1 plays an important role and can be a potent marker of vascular disorders, including atherosclerosis, as well as of angiogenesis [16, 17].…”

Section: Introductionmentioning

confidence: 99%

Soluble Form of Vascular Cell Adhesion Molecule 1 Induces Migration and Proliferation of Vascular Smooth Muscle Cells

Lee¹,

Kim²,

Won³

et al. 2008

J Vasc Res

View full text Add to dashboard Cite

Background: Serum levels of soluble vascular cell adhesion molecule 1 (sVCAM-1) shed from its membrane-bound form are elevated in hypertension. This study clarified the effects of sVCAM-1 on vascular responses in rat aortic smooth muscle cells (RASMCs). Methods: Boyden chamber, 5-bromo-2′-deoxyuridine incorporation and ex vivo aortic ring assays for migration and proliferation, and Western blot for the kinase activity were used. Results: Spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats were compared functionally. sVCAM-1 increased RASMC migration and proliferation, which were greater in SHR compared with WKY rats. RASMCs expressed the very late antigen 4α receptor integrin with no difference between SHR and WKY rats. Inhibitors of phosphoinositide kinase 3 (PI3K) and spleen tyrosine kinase (Syk) and small interference RNA-Syk abolished the sVCAM-1-induced migration, proliferation and phosphorylation of focal adhesion kinase. The phosphorylation of Syk was significantly greater in RASMCs from SHR than from WKY rats. sVCAM-1 increased aortic sprout outgrowth, which was inhibited by inhibitors of PI3K and Syk. Conclusions: This study suggests that sVCAM-1 promotes the RASMC migration and proliferation via the focal adhesion kinase pathway regulated by Syk and PI3K, and the altered sVCAM-1-induced responses during hypertension are closely associated with the increments in intracellular signal transmission.

show abstract

“…On the other hand, an association between the serum levels of sVCAM-1 and arterial blood pressure has been reported (24), and is corroborated by our study. This association could trigger the development of certain systemic failures, which could increase cardiovascular risk (7,25).…”

Section: Discussionsupporting

confidence: 92%

Increased concentrations of soluble vascular cell adhesion molecule-1 and soluble CD40L in subjects with metabolic syndrome

Palomo

Jaramillo²,

Alarcón³

et al. 2009

Mol Med Rep

View full text Add to dashboard Cite

Abstract. Metabolic syndrome (MS) is associated with a high incidence rate of cardiovascular disease. It is characterized by abdominal obesity, elevated blood pressure, atherogenic dyslipidemia [high LDL-c (low density lipoprotein cholesterol) and low HDL-c (high density lipoprotein cholesterol)] and insulin resistance or glucose intolerance. In the context of MS, alterations in the plasmatic levels of some soluble forms of cell adhesion molecules can appear, e.g., soluble vascular cell adhesion molecule-1 (sVCAM-1), soluble E-selectin (sE-selectin) and soluble CD40L (sCD40L). The objective of this study was to compare the serum levels of sVCAM-1, sE-selectin and sCD40L in MS and non-MS groups and to associate these molecules with the diagnostic criteria of MS. A total of 185 non-smokers between 45 and 64 years of age were included. Of these, 93 corresponded to the MS group and the remaining 92 to a non-MS group (according to modified ATP III criteria). The serum concentration of sVCAM-1, sE-selectin and sCD40L was determined by commercial solid phase ELISA. The results were expressed as a median and interquartile range. The MS group showed high levels of sVCAM-1 (558.9 ng/ml; 481.3-667.6 ng/ml) compared with the non-MS group (405.2 ng/ml; 361.0-470.5 ng/ml) (p<0.0001). As well, the median level of sCD40L (3.0 ng/ml; 2.1l-11.7 ng/ml) was significantly higher in the MS group than that in the non-MS group (2.6 ng/ml; 2.3-3.4 ng/ml) (p=0.0061). sE-selectin levels did not differ significantly between the groups: 73.9 ng/ml (58.3-87.0 ng/ml) and 68.5 ng/ml (51.6-97.5 ng/ml) in the MS and non-MS group, respectively. In conclusion, the serum levels of sVCAM-1 and sCD40L, but not sE-selectin, were significantly higher in patients with MS than in subjects that did not present MS. MS may therefore increase the expression of cell adhesion molecules, probably through endothelial activation. IntroductionMetabolic syndrome (MS) is a cluster of cardiovascular risk factors including central obesity, hypertension, dyslipidemias and glucose intolerance (1). Clinically, it has reached epidemic proportions, and with an increase in the elderly population its incidence and prevalence will further multiply (2).Visceral adipose tissue, an important feature in individuals with MS, produces a range of circulating molecules with proinflammatory and pro-atherosclerotic actions. Several of these adipokines, including tumour necrosis factor · (TNF-·) and interleukin 6 (IL-6), have been linked to alterations in endothelial functions (3).An alteration that characterizes endothelial dysfunction involves the secretion of cellular adhesion molecules (CAMs) on the surface of endothelial cells (ECs), thus enabling the CAMs to bind leukocytes (4,5). VCAM-1 (vascular cell adhesion molecule-1) is not expressed at high levels on the endothelium, but can be regulated in vitro in response to TNF-·, IL-4 and interferon-Á (IFN-Á) cytokines that are synthesized by adipose as well as other tissues. Soluble VCAM-1 (sVCAM-1) is found in the serum of healthy pe...

show abstract

Plasma levels of soluble cellular adhesion molecules in patients with arterial hypertension. Correlations with plasma endothelin-1

Cited by 40 publications

References 22 publications

The Influence of Hyperlipidemia on Platelet Activity Markers in Patients after Ischemic Stroke

The Influence of Hyperlipidemia on Platelet Activity Markers in Patients after Ischemic Stroke

Soluble Form of Vascular Cell Adhesion Molecule 1 Induces Migration and Proliferation of Vascular Smooth Muscle Cells

Increased concentrations of soluble vascular cell adhesion molecule-1 and soluble CD40L in subjects with metabolic syndrome

Contact Info

Product

Resources

About