Plasma Lipidomics Investigation of Hemodialysis Effects by Using Liquid Chromatography–Mass Spectrometry

Wang, Lichao; Hu, Chunxiu; Liu, Shuxin; Ming, Chang; Gao, Peng; Wang, Lili; Pan, Zaifa; Xu, Guowang

doi:10.1021/acs.jproteome.6b00170

Cited by 34 publications

(29 citation statements)

References 51 publications

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“…Further analysis found that polyunsaturated fatty acids (PUFA) including DHA, DTA and TTA were dramatically decreased and saturated fatty acids (SFA), such as 5,8-TDA and EDA were obviously increased in CKD patients with microalbuminuria and macroalbuminuria. This was consistent with the earlier studies that plasma free fatty acids and SFA were significantly elevated in the pre-hemodialysis samples of ESRD patients compared with healthy controls [51]. We also identified DTA and DHA in the adenine-induced rats with CKD in our previous studies [52,53].…”

Section: Tablesupporting

confidence: 92%

Activated NF-κB/Nrf2 and Wnt/β-catenin pathways are associated with lipid metabolism in CKD patients with microalbuminuria and macroalbuminuria

Feng

Chen

et al. 2019

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Early diagnosis of CKD patients at risk for microalbuminuria or macroalbuminuria could facilitate clinical outcomes and long-term survival. Considering the few and limited efficacy of current biomarkers in early detection, we aim to discover plasma lipids that effectively predict the development of CKD paitents with microalbuminuria or macroalbuminuria. A total of 380 healthy controls and 1156 patients with CKD stages 3 to 5 were stratified by urine albumin-creatinine ratio as microalbuminuria (30-300 mg/g) and macroalbuminuria (> 300 mg/g). Fasting plasma samples were determined by UPLC-HDMS based on lipidomics. Quantitative realtime polymerase chain reaction, Western blot and immunohistochemical analyses were used to validate the lipid metabolism-associated pathways. Pathway analysis demonstrated that these lipids were closely associated with PPARγ, inflammatory mediator regulation of TRP channels and RAS signaling, which were intimately involved in activated NF-κB and Nrf2 pathways. We further carried out pathway validation and demonstrated that NF-κB pathway was activated in patients with macroalbuminuria compared with CKD patients with microalbuminuria, while Nrf2-associated protein expression was downregulated, which was accompanied by the up-regulation of Wnt/β-catenin signaling pathway. Four lipids including DTA, 5,8-TDA, GGD3 and DHA that showed great potential in the discrimination of CKD patients with microalbuminuria and healthy controls were selected by logistic regression analysis. Additionally, six lipid species including CDCA, glucosylceramide, GGD2, TTA, DHA and EDA that contributed to the discrimination of CKD patients with microalbuminuria and macroalbuminuria were selected by logistic LASSO regression Gangliosides were first identified and might be promising therapeutic targets for CKD patients with the different degree of albuminuria. Collectively, this study first demonstrates the

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Section: Tablesupporting

confidence: 92%

Activated NF-κB/Nrf2 and Wnt/β-catenin pathways are associated with lipid metabolism in CKD patients with microalbuminuria and macroalbuminuria

Feng

Chen

et al. 2019

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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“…Lipidome in liver (mice) and plasma (mice and human) were analysed by Liquid Chromatography-Mass Spectrometry (LC-MS) based on previous methods. [14][15][16] More details are described in the supporting information section.…”

Section: Lipid Profilingmentioning

confidence: 99%

Coordinated changes of gut microbiome and lipidome differentiates nonalcoholic steatohepatitis (NASH) from isolated steatosis

Qian

Yao

et al. 2019

Liver International

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Background & Aims Nonalcoholic fatty liver disease encompasses isolated steatosis or nonalcoholic fatty liver and nonalcoholic steatohepatitis (NASH). NASH develops from isolated steatosis with obscure driving forces. We aim to identify key factors promoting this transition. Methods Following 21‐week of high‐fat diet feeding, obese mice were classified into two groups termed as isolated steatosis and NASH based on hematoxylin‐eosin staining of liver histology. The integrated multi‐omics analysis of lipidome, transcriptome and gut microbiome were performed in mice with isolated steatosis and NASH, and confirmed in human samples. Results Livers in mice with NASH lost most lipids, and the transcriptional landscape was also changed dramatically in mice with NASH in relative to mice with isolated steatosis. Plasma lipidome analysis demonstrated a very clear difference between these two groups of mice, which was partially recapitulated in serum of patients with isolated steatosis and NASH. The microbiota composition revealed that Bacteroides genus and Bacteroides uniformis species decreased while Mucispirillum genus and Mucispirillum schaedleri species increased largely in mice with NASH. More importantly, we found that Bacteroides uniformis correlated positively with triglycerides (TGs) and negatively with free fatty acids (FFAs) and PE(18:1/20:4), while Mucispirillum schaedleri correlated positively with FFAs, LysoPC(20:3), LysoPC(20:4) and DG(16:1/18:2). Mechanically, administration of Bacteroides uniformis increased specific TGs, and decreased hepatic injury and inflammation in diet‐induced mice. Conclusions Overall, through multi‐omics integration, we identified a microbiota‐lipid axis promoting the initiation of NASH from isolated steatosis, which might provide a novel perspective on NASH pathogenesis and treatment.

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“…The mobile-phase additives commonly employed for lipidomics analysis in ESI − include 5-10 mM AmAc, 12,14,22,23 5-20 mM AmF, [24][25][26] 0.02-0.1% AA, 27,28 0.2% FA 12,27 and combinations of these salts and acids. 29 The use of 0.05% AmOH was shown to give a higher signal for the anionic phospholipids PG, PA, PS and PI (including their lyso forms) and the zwitterionic PE when compared to 10 mM AmAc.…”

Section: For Lipidomics Studies Such As Those Of Quehnberger Et Al 20mentioning

confidence: 99%

Improving negative liquid chromatography/electrospray ionization mass spectrometry lipidomic analysis of human plasma using acetic acid as a mobile‐phase additive

Monnin

Ramrup

Daigle-Young

et al. 2018

Rapid Comm Mass Spectrometry

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Plasma Lipidomics Investigation of Hemodialysis Effects by Using Liquid Chromatography–Mass Spectrometry

Cited by 34 publications

References 51 publications

Activated NF-κB/Nrf2 and Wnt/β-catenin pathways are associated with lipid metabolism in CKD patients with microalbuminuria and macroalbuminuria

Activated NF-κB/Nrf2 and Wnt/β-catenin pathways are associated with lipid metabolism in CKD patients with microalbuminuria and macroalbuminuria

Coordinated changes of gut microbiome and lipidome differentiates nonalcoholic steatohepatitis (NASH) from isolated steatosis

Improving negative liquid chromatography/electrospray ionization mass spectrometry lipidomic analysis of human plasma using acetic acid as a mobile‐phase additive

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