Plasma lipidomics reveal profound perturbation of glycerophospholipids, fatty acids, and sphingolipids in diet-induced hyperlipidemia

Miao, Hua; Chen, Hua; Pei, Songwen; Xu, Bo; Vaziri, Nosratola D.; Zhao, Ying-Yong

doi:10.1016/j.cbi.2015.01.023

Cited by 82 publications

(70 citation statements)

References 80 publications

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“…In our sample of women, LysoPC C18:2 and other LysoPC species are included in PCA Factor 8, which was exclusively correlated with adiposity and BMI instead of insulin resistance measured with HOMA-IR. Miao et al (30) reported that some LysoPC species were increased, while others were lowered, in hyperlipidemic rats fed a high-fat diet for 6 wk. Another study in mice fed a high-fat diet showed elevation in blood and liver of PC species that were associated with increased fat accumulation whereas most LysoPCs were decreased and some others were increased (17).…”

Section: E743mentioning

confidence: 99%

“…Sun et al (43) observed high blood PC levels as well as elevated cholesterol and triglyceride concentrations in pigs fed a high-fat diet without weight gain compared with animals fed a control diet. Lysophosphatidylcholines (lysoPC) are hydrolyzed derivatives of PC, and studies have demonstrated that these compounds play a role in the development of atherosclerosis and hyperlipidemia (27,30). They are components of oxidized LDL cholesterol particles, which are related to the risk of cardiovascular disease (16,26).…”

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confidence: 99%

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Alterations of plasma metabolite profiles related to adipose tissue distribution and cardiometabolic risk

Boulet

Chevrier

Grenier-Larouche

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

117

106

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profiling of obese individuals revealed altered concentrations of many metabolites, especially branched-chain amino acids (BCAA), possibly linked to altered adipose tissue BCAA catabolism. We tested the hypothesis that some features of this metabolite signature relate closely to visceral obesity and concomitant alterations in cardiometabolic risk factors. We also postulated that alterations in BCAA-catabolizing enzymes are predominant in visceral adipose tissue. Fifty-nine women (BMI 20 -41 kg/m 2 ) undergoing gynecologic surgery were recruited and characterized for overall and regional adiposity, blood metabolite levels using targeted metabolomics, and cardiometabolic risk factors. Adipose samples (visceral and subcutaneous) were obtained and used for gene expression and Western blot analyses. Obese women had significantly higher circulating BCAA and kynurenine/tryptophan (Kyn/Trp) ratio than lean or overweight women (P Ͻ 0.01). Principal component analysis confirmed that factors related to AA and the Kyn/Trp ratio were positively associated with BMI, fat mass, visceral or subcutaneous adipose tissue area, and subcutaneous adipocyte size (P Յ 0.05). AA-related factor was positively associated with HOMA-IR (P Յ 0.01). Factors reflecting glycerophospholipids and sphingolipids levels were mostly associated with altered blood lipid concentrations (P Յ 0.05). Glutamate level was the strongest independent predictor of visceral adipose tissue area (r ϭ 0.46, P Ͻ 0.001). Obese women had lower expression and protein levels of BCAA-catabolizing enzymes in visceral adipose tissue than overweight or lean women (P Յ 0.05). We conclude that among metabolites altered in obesity plasma concentrations of BCAA and the Kyn/Trp ratio are closely related to increased adiposity. Alterations in expression and protein levels of BCAA-catabolizing enzymes are predominant in visceral adipose tissue.

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Section: E743mentioning

confidence: 99%

mentioning

confidence: 99%

Alterations of plasma metabolite profiles related to adipose tissue distribution and cardiometabolic risk

Boulet

Chevrier

Grenier-Larouche

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

117

106

View full text Add to dashboard Cite

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“…Furthermore, as most lipid species are non-volatile and tend to degrade at high temperatures used in GC, liquid chromatography (LC) systems, coupled to MS detection emerged as powerful tools in lipidomics applications [35,36]. Besides commonly used high performance liquid chromatography (HPLC) systems with normal and reverse phase columns, UHPLC (ultra high performance chromatography), operating at pressures up to 15,000 psi now provide better mass resolution, enhanced sensitivity and greater signal-to-noise ratio [37,38,39]. The chromatographic separation of lipid species reduces ion suppression by reducing the number of competing analytes, entering the MS system at the same time.…”

Section: Analytical Approaches To Study Lipidsmentioning

confidence: 99%

Lipidomics—Reshaping the Analysis and Perception of Type 2 Diabetes

Markgraf

Al-Hasani

Lehr

2016

IJMS

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As a consequence of a sedentary lifestyle as well as changed nutritional behavior, today’s societies are challenged by the rapid propagation of metabolic disorders. A common feature of diseases, such as obesity and type 2 diabetes (T2D), is the dysregulation of lipid metabolism. Our understanding of the mechanisms underlying these diseases is hampered by the complexity of lipid metabolic pathways on a cellular level. Furthermore, overall lipid homeostasis in higher eukaryotic organisms needs to be maintained by a highly regulated interplay between tissues, such as adipose tissue, liver and muscle. Unraveling pathological mechanisms underlying metabolic disorders therefore requires a diversified approach, integrating basic cellular research with clinical research, ultimately relying on the analytical power of mass spectrometry-based techniques. Here, we discuss recent progress in the development of lipidomics approaches to resolve the pathological mechanisms of metabolic diseases and to identify suitable biomarkers for clinical application. Due to its growing impact worldwide, we focus on T2D to highlight the key role of lipidomics in our current understanding of this disease, discuss remaining questions and suggest future strategies to address them.

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“…Apart from changes in steroid metabolites profile, increased levels of citric acid cycle intermediates and lipid β-oxidation products were observed in all DCM patients [69]. Taking into consideration multiplicity of lipid fractions and their various physiological functions, current clinical serum lipids profile examination does not provide detailed information on lipids metabolism abnormalities [70]. Therefore, according to enhanced lipolysis and increased free FA plasma level observed in HF patients, it is vital to distinguish the metabolism of which particular lipid fraction is dysregulated.…”

Section: Heart Failure -A Multisystemic Diseasementioning

confidence: 97%

Metabolomics — A wide-open door to personalized treatment in chronic heart failure?

Marcinkiewicz-Siemion

Ciborowski

Krętowski

et al. 2016

International Journal of Cardiology

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Plasma lipidomics reveal profound perturbation of glycerophospholipids, fatty acids, and sphingolipids in diet-induced hyperlipidemia

Cited by 82 publications

References 80 publications

Alterations of plasma metabolite profiles related to adipose tissue distribution and cardiometabolic risk

Alterations of plasma metabolite profiles related to adipose tissue distribution and cardiometabolic risk

Lipidomics—Reshaping the Analysis and Perception of Type 2 Diabetes

Metabolomics — A wide-open door to personalized treatment in chronic heart failure?

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