Plasma lncRNA GAS8-AS1 as a Potential Biomarker of Papillary Thyroid Carcinoma in Chinese Patients

Zhang, Dongxue; Liu, Xin; Wei, Bojun; Qiao, Guoliang; Jiang, Tao; Chen, Zhenwen

doi:10.1155/2017/2645904

Cited by 33 publications

(22 citation statements)

References 25 publications

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“…2 Expression of GAS8-AS1 predicted survival. Survival curve analysis showed that downregulated GAS8-AS1 in OS tissues was closely correlated with poor survival Discussion lncRNA GAS8-AS1 has been characterized as a tumor suppressor in papillary thyroid carcinoma [11,12]. The key finding of the present study is that GAS8-AS1 is downregulated in OS and may play a role as tumor suppressor in OS by downregulating lncRNA UCA1, which promotes OS progression.…”

Section: Gas8-as1 Inhibited Os Cell Migration and Invasion Through Uca1supporting

confidence: 52%

“…In recent years, circulating biomarkers, such as blood circulating lncRNAs have been attracting more research attentions due to the non-invasive nature [18]. A recent study reported that plasma GAS8-AS1 was downregulated in Chinese patients with papillary thyroid carcinoma, and downregulated GAS8-AS1 provided novel diagnostic insights [12]. Our study showed that low expression level of GAS8-AS1 in OS tissue is closely correlated with the poor survival of OS patients.…”

Section: Gas8-as1 Inhibited Os Cell Migration and Invasion Through Uca1mentioning

confidence: 52%

“…Therefore, regulation of lncRNAs expression may indirectly contribute to cancer prevention and treatment [10]. lncRNA GAS8-AS1 has been characterized as a tumor suppressor in papillary thyroid carcinoma and colorectal cancer [11][12][13]. Our preliminary deep sequencing data revealed that GAS8-AS1 was downregulated in OS and was inversely correlated with lncRNA UCA1, which has been identified as an oncogenic lncRNA in OS [14].…”

Section: Introductionmentioning

confidence: 82%

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lncRNA GAS8-AS1 downregulates lncRNA UCA1 to inhibit osteosarcoma cell migration and invasion

et al. 2020

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Background: Osteosarcoma (OS) is the most common type of primary bone tumor that mainly affects adolescents and young adults. The present study explored the role of lncRNA GAS8-AS1 in OS. Methods: A total of 48 OS patients were selected from the 82 OS patients admitted by Luoyang Orthopedic Hospital of Henan Province between May 2010 and May 2013. Transient cell transfections, Transwell cell migration and invasion assay, RT-qPCR, and patient follow-up were carried out during the research. Results: The results showed that GAS8-AS1 was downregulated, while UCA1 was upregulate in cancer tissues in comparison to adjacent non-cancer tissues of OS patients. GAS8-AS1 was not affected by clinical stage. Follow-up study showed that downregulated GAS8-AS1 in cancer tissues was closely correlated with poor survival. GAS8-AS1 and UCA1 were inversely correlated in cancer tissues. Overexpression of UCA1 failed to affect the expression of GAS8-AS1, while overexpression of GAS8-AS1 led to downregulated expression of UCA1 in OS cells, while the molecular mediators between these two lncRNAs are unknown. Overexpression of GAS8-AS1 did not affect OS cell proliferation but significantly inhibited cancer cell migration and invasion. Overexpression of UCA1 promoted the migration and invasion of OS cells and attenuated the effects of overexpressing GAS8-AS1. Conclusions: Therefore, GAS8-AS1 may inhibit OS cell migration and invasion by downregulating oncogenic UCA1.

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Section: Gas8-as1 Inhibited Os Cell Migration and Invasion Through Uca1supporting

confidence: 52%

Section: Gas8-as1 Inhibited Os Cell Migration and Invasion Through Uca1mentioning

confidence: 52%

Section: Introductionmentioning

confidence: 82%

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lncRNA GAS8-AS1 downregulates lncRNA UCA1 to inhibit osteosarcoma cell migration and invasion

et al. 2020

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“…The incidence of thyroid cancer has increased dramatically over the last several decades [ 23 ], and thus the risk of ITC is an important component of the decision concerning the optimal operation in NTMNG. Its incidence in NTMNG is estimated at 4–18% [ 22 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Nontoxic Multinodular Goitre and Incidental Thyroid Cancer: What Is the Best Surgical Strategy?—A Retrospective Study of 2032 Patients

Kaliszewski

Wojtczak

Grzegrzółka³

et al. 2018

International Journal of Endocrinology

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Objectives A discussion with regard to the most optimal surgical procedure in nontoxic multinodular goitre (NTMNG). We assessed and compared three main types of operations in 2032 patients with NTMNG. Methods This is a retrospective study of 2032 patients operated on in one center due to NTMNG. The observation period was 48 to 120 months (mean and SD: 87 ± 20). Results The early complications included uni- and bilateral recurrent laryngeal nerve paralysis (URLNP, BRLNP), overt tetany (OT), and postoperative bleeding (POB). We observed after TT, STT, and DO URLNP: 15 (1.73%), 3 (0.64%), and 2 (0.28%), respectively (p < 0.05); BRLNP: 3 (0.34%), 2 (0.43%), and 0 (0.0%), respectively (p = 0.25); OT: 42 (4.84%), 6 (1.29%), and 9 (1.28%), respectively (p < 0.0001); and POB: 11 (1.26%), 4 (0.86%), and 3 (0.42%), respectively (p = 0.209). Persistent complications we observed after TT, STT, and DO are the following: URLNP: 9 (1.03%), 3 (0.64%), and 1 (0.14%), respectively (p = 0.086); BRLNP: 1 (0.11%), 1 (0.21%), and 0 (0.0%), respectively (p = 0.51); and OT: 11 (1.26%), 2 (0.43%), and 2 (0.28%), respectively (p = 0.052). Incidental thyroid cancer (ITC) was recognized after TT, STT, and DO in 18 (2.07%), 21 (4.52%), and 11 (1.56%), respectively (p = 0.039). Recurrent goitre (RG) was observed in 1 (0.11%) after TT, 3 (0.64%) after STT, and 2 (0.28%) after DO (p = 0.234). Conclusions Performing less radical surgery in NTMNG is associated with a significantly lower risk of early and late URLNP and OT. In the case of BRLNP (early and persistent) and POB, no statistically significant differences are seen. The occurrence of ITC is higher following more radical surgeries. Less radical surgery is connected with a higher risk of RG.

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“…LncRNAs play a crucial part in transcriptional regulation, epigenetic gene regulation and disease [8]. And many lncRNAs were convinced of having connected with thyroid carcinoma [29][30][31]. The study shows that LncRNA LINC00511 promotes osteosarcoma through miR-765 [32].…”

Section: Discussionmentioning

confidence: 97%

LINC00511 influences cellular proliferation through cyclin-dependent kinases in papillary thyroid carcinoma

Xiang¹,

Guan²,

Bhandari³

et al. 2020

J. Cancer

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Background: Proverbially, the incidence rate of papillary thyroid carcinoma (PTC) has increased year by year. Many long noncoding RNAs (lncRNAs) have been discovered having a relationship with tumor genesis tightly recently. Thanks to the previous researches, we found long intergenic noncoding RNA 00511 (LINC00511) is overexpressed and acts as an oncogene in non-small-cell lung cancer and breast cancer. However, the biological role and function of LINC00511 are still unclear in PTC. Methods: We got the expression of LINC00511 in PTC tissues and matched adjacent tissues, as well the cell lines (B-CPAP, KTC-1, and KTC-1) by way of quantitative real-time polymerase chain reaction (qRT-PCR). In vitro, we knocked down the LINC00511 with small interfering RNA in PTC cell lines and demonstrated the function of LINC00511 by Cell Counting Kit-8, cell colony formation, Transwell migration, Transwell invasion, apoptosis assays, and cell cycle assays. Then, we discovered several downstream proteins of LINC00511 using Western blotting. Results: We proved that LINC00511's expression in PTC tissues and cell lines is higher than the control. LINC00511 promoted cellular proliferation, migration, invasion, G1/S transition and reduced apoptosis in vitro experiment. Knocked-down of LINC00511 resulted in the reduction of histone methyltransferase enhancer of zeste homolog 2 (EZH2), cyclin-dependent kinase 2 (CDK2) and cyclin-dependent kinase 4 (CDK4). Conclusions: Our results certified the role and function of LINC00511 in PTC, and it could become a novel tumor therapeutic target.

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Plasma lncRNA GAS8-AS1 as a Potential Biomarker of Papillary Thyroid Carcinoma in Chinese Patients

Cited by 33 publications

References 25 publications

lncRNA GAS8-AS1 downregulates lncRNA UCA1 to inhibit osteosarcoma cell migration and invasion

lncRNA GAS8-AS1 downregulates lncRNA UCA1 to inhibit osteosarcoma cell migration and invasion

Nontoxic Multinodular Goitre and Incidental Thyroid Cancer: What Is the Best Surgical Strategy?—A Retrospective Study of 2032 Patients

LINC00511 influences cellular proliferation through cyclin-dependent kinases in papillary thyroid carcinoma

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