Plasma low-molecular-weight thiol/disulphide homeostasis as an early indicator of global and focal cerebral ischaemia

Ivanov, Alexander; Александрин, В. В.; Pal'tsyn, A. A.; Nikiforova, Ksenia; Virus, Edward Danielevich; Luzyanin, B. P.; Maksimova, M. Yu.; Piradov, М. А.; Кубатиев, Аслан Амирханович

doi:10.1080/13510002.2017.1311464

Cited by 22 publications

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“…The resulting disulfide bond structures are again reduced to thiol groups so that the thiol/disulfide balance is maintained [ 7 ]. Diseases including diabetes mellitus [ 8 ], obesity [ 9 ], cardiovascular disease [ 10 ], hypertension [ 11 ], malignancy [ 12 ], rheumatic disease, chronic renal failure [ 13 ], Alzheimer's disease [ 14 ], chronic liver disease [ 15 ], cerebral ischemia [ 16 ], nasal polyposis [ 17 ], preeclampsia [ 18 ], uterine myoma [ 19 ], cataracts [ 20 ], chronic radiation exposure [ 21 ], and acute tonsillopharyngitis [ 22 ] have been assessed by the same method used in our study, and a close relationship with oxidative stress was detected. A strong association between familial hypercholesterolemia and premature atherosclerotic cardiovascular disease was established in a study conducted with 1,690 patients [ 1 ], and, in our study, we found that disulfide formation was elevated in patients with familial hypercholesterolemia.…”

Section: Discussionmentioning

confidence: 99%

Thiol/Disulfide Balance in Patients with Familial Hypercholesterolemia

Özdemir-Şimşek

Çarlıoğlu

Alışık

et al. 2018

Cardiology Research and Practice

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Objective Herein, we investigated the balance of thiol/disulfide, with the hypothesis that the balance between disulfides and thiols, which are natural antioxidants, might be disrupted in patients with familial hypercholesterolemia, which eventually leads to endothelial damage. Methods In this study, we evaluated 51 patients diagnosed with familial hypercholesterolemia and 81 healthy subjects. Blood samples were taken from the patients after a minimum of 12 hours of fasting; samples were immediately centrifuged, stored in Eppendorf tubes, and preserved at −80°C. Results This study found that thiol levels are significantly lower in patients with familial hypercholesterolemia, whereas disulfide levels are higher (independent of age, gender, and body mass index). This means that in such patients, the thiol/disulfide balance changes in favour of disulfide. Conclusions In this study, we found that the thiol/disulfide balance in patients with familial hypercholesterolemia is disrupted in favour of disulfide.

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Section: Discussionmentioning

confidence: 99%

Thiol/Disulfide Balance in Patients with Familial Hypercholesterolemia

Özdemir-Şimşek

Çarlıoğlu

Alışık

et al. 2018

Cardiology Research and Practice

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“…The close relationship between the plasma and cerebral LMWTs pools has been shown in experimental models of cerebral ischemia. In first, a decrease in RS of LMWTs in plasma was detected in a model of acute focal and global brain ischemia, that was accompanied by a decrease in rGSH content or an increase in oxidized GSH in brain tissue [ 17 , 18 ]. Secondly, the ability of GSH to alleviate of brain injury when it is injected into the blood was shown in the model of transient focal cerebral ischemia [ 25 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…The samples were mixed thoroughly, frozen, and stored at –80°C. The t-, r-forms, and the RS of Cys, Hcy, and GSH were determined by liquid chromatography as previously described [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

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Impact of glutathione on acute ischemic stroke severity and outcome: possible role of aminothiols redox status

et al. 2021

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Objective: Acute brain ischemia is accompanied by a disruption of low-molecular-weight aminothiols (LMWTs) homeostasis, such as homocysteine (Hcy), cysteine (Cys), and glutathione (GSH). We investigated the redox balance of LMWTs in blood plasma and its influence on ischemic stroke severity and the functional outcome in patients with an acute period. Patients and methods: A total of 177 patients were examined. Total and reduced forms of LMWTs were determined in the first 10-24 h. Stroke severity and functional state were estimated using the National Institutes of Health Stroke Scale (NIHSS) and the modified Rankin Scale (mRs) at admission and after 21 days. Results: Patients with high levels of total Hcy (> 19 μM) showed significantly reduced redox statuses of all LMWTs. Patients with low total GSH levels (≤ 1.07 μM) were at an increased risk of higher stroke severity (NIHSS > 10) compared to patients with a total GSH level > 2.64 μM (age/gender-adjusted odds ratio: 4.69, 95% CI: 1.43-15.4). Discussion: (1) low total GSH level can be considered as a novel risk marker for the severity of acute stroke in conditions of low redox status of LMWTs and (2) high Hcy levels associated with low redox status of LMWTs.

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“…Эксперименты проводили на наркотизированных хлоралгидратом (300 мг/кг, в/б) беспородных крысах-самцах (n = 20) массой 260-300 г. Мозговой кровоток регистрировали в теменном неокортексе лазерным допплеровским флоуметром ЛАКК-02 (Лазма, Россия) по описанной ранее методике [3]. Регистрацию системного АД в бедренной артерии осуществляли монитором MEC8 (Mindray, Chine).…”

Section: материал и методы исследованияunclassified

The scale of cerebral ischemia thresholds in rats

Александрин¹

2018

Nauchno-Prakticheskii Zhurnal «Patogenez»

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Прогноз и тактика лечения инсульта зависят от его тяжести, которая задается достигнутым ишемическом порогом. Исследование механизмов формирования ишемических порогов является актуальной патофизиологической задачей. Целью настоящего исследования явилось получение данных для построения шкалы порогов ишемии мозга у крыс. Эксперименты проводили на наркотизированных беспородных крысах-самцах. Мозговой кровоток регистрировали лазерным допплеровским флоуметром ЛАКК-02 (Лазма, Россия). Контролируемую кровопотерю осуществляли через бедренную артерию. Фиксировали системное артериальное давление (АД), при котором в условиях медленной кровопотери регистрировалось пороговое значение мозгового кровотока. На основании полученных данных строилась шкала: системное АД - кровоток - пороги ишемии мозга. Prognosis and treatment of stroke depend on its severity, which is defined by the attained ischemic threshold. Studying the mechanisms underlying the formation of ischemic thresholds is an urgent pathophysiological task. The aim of this study was to obtain data for constructing a scale of cerebral ischemia thresholds in rats. Experiments were carried out on anesthetized mongrel male rats. Cerebral blood flow was recorded with a laser Doppler flowmeter, LAKK-02 (Lazma, Russia). Controlled blood loss was performed through the femoral artery. Systemic blood pressure, at which the threshold value of cerebral blood flow was attained, was recorded under the conditions of slow blood loss. Based on the obtained data the systemic blood pressure - blood flow - thresholds of cerebral ischemia scale was constructed.

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Plasma low-molecular-weight thiol/disulphide homeostasis as an early indicator of global and focal cerebral ischaemia

Abstract: These results suggest that plasma low-molecular-weight thiols are actively involved in oxidation reactions at early stages of cerebral ischaemia; therefore, their reduced forms or redox state may serve as a sensitive indicator of acute cerebrovascular insufficiency.

Cited by 22 publications

References 38 publications

Thiol/Disulfide Balance in Patients with Familial Hypercholesterolemia

Thiol/Disulfide Balance in Patients with Familial Hypercholesterolemia

Impact of glutathione on acute ischemic stroke severity and outcome: possible role of aminothiols redox status

The scale of cerebral ischemia thresholds in rats

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