Plasma membrane transport systems in higher plants: From black boxes to molecular physiology

Logan, Helen; Basset, Mireille; Véry, Anne‐Aliénor; Sentenac, Hervé

doi:10.1034/j.1399-3054.1997.1000101.x

Cited by 18 publications

(25 citation statements)

References 59 publications

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“…In recent years, simultaneous advances in electrophysiological and molecular approaches have brought about a large body of information on the transport of ions across plant cell membranes (Barkla and Pantoja, 1996;Logan et al, 1997). The mechanisms of non-electrolyte transport have been investigated to a much lesser extent and concern mostly the PM level.…”

Section: Introductionmentioning

confidence: 99%

Aquaporin Nt‐TIPa can account for the high permeability of tobacco cell vacuolar membrane to small neutral solutes

et al. 1999

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SummaryMembers of the major intrinsic protein (MIP) family, described in plants as water-selective channels (aquaporins), can also transport small neutral solutes in other organisms. In the present work, we characterize the permeability of plant vacuolar membrane (tonoplast; TP) and plasma membrane (PM) to non-electrolytes and evaluate the contribution of MIP homologues to such transport. PM and TP vesicles were puri®ed from tobacco suspension cells by free-¯ow electrophoresis, and membrane permeabilities for a wide range of neutral solutes including urea, polyols of different molecular size, and amino acids were investigated by stopped-¯ow spectro¯uorimetry. For all solutes tested, TP vesicles were found to be more permeable than their PM counterparts, with for instance urea permeabilities from in¯ux experiments of 74.9 T 9.6 Q 10 ±6 and 1.0 T 0.3 Q 10 ±6 cm sec ±1 , respectively. Glycerol and urea transport in TP vesicles exhibited features of a facilitated diffusion process. This and the high channel-mediated permeability of the same TP vesicles to water suggested a common role for MIP proteins in water and solute transport. A cDNA encoding a novel tonoplast intrinsic protein (TIP) homologue named Nicotiana tabacum TIPa (Nt-TIPa) was isolated from tobacco cells. Immunodetection of Nt-TIPa in puri®ed membrane fractions con®rmed that the protein is localized in the TP. Functional expression of Nt-TIPa in Xenopus oocytes showed this protein to be permeable to water and solutes such as urea and glycerol. These features could account for the transport selectivity pro®le determined in puri®ed TP vesicles. These results support the idea that plant aquaporins have a dual function in water and solute transport. Because Nt-TIPa diverges in sequence from solute permeable aquaporins characterized in other organisms, its identi®cation also provides a novel tool for investigating the molecular determinants of aquaporin transport selectivity.

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Section: Introductionmentioning

confidence: 99%

Aquaporin Nt‐TIPa can account for the high permeability of tobacco cell vacuolar membrane to small neutral solutes

et al. 1999

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“…The two abundant N-sources (NO 3 − and NH 4 + ) which are frequently used by plants are usually taken up via specialised carriers in the plasma membrane and tonoplast (Raven and Farquhar 1981, Miller and Smith 1996, Logan et al 1997. Inhibitor experiments to investigate the mechanism of NH 3induced [Ca 2 + ] c responses in Arabidopsis roots.…”

Section: Discussionmentioning

confidence: 99%

Ammonium uptake and cellular alkalisation in roots of Arabidopsis thaliana: The involvement of cytoplasmic calcium

Plieth¹,

Sattelmacher²,

Knight³

2000

Physiol Plant

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Using roots from Arabidopsis thaliana expressing the recom-contribute significantly to the NH 3 -triggered [Ca 2 + ] c response. Pre-incubation in L-methionine-DL-sulphoximine -an binant calcium indicator aequorin, we show that NH 3 uptake and alkalisation of plant cells act as a stimulus which induces inhibitor of the glutamine synthetase -did not alter the transient elevations of the cytoplasmic free calcium concentra-NH 3 -induced [Ca 2 + ] c responses at all. These results are consistent with previous studies where NH 3 -induced changes of tion ([Ca 2 + ] c ). The magnitudes of these [Ca 2 + ] c elevations are dependent on the concentration of the membrane perme-cytoplasmic and vacuolar pH were investigated in maize roots. Furthermore, the similarity between the kinetics of NH 3 -able form, NH 3 , and hence, particularly dependent on the pH in the external medium. EGTA and La 3 + are able to signifi-driven cellular pH changes demonstrated in previous studies and the [Ca 2 + ] c transients shown here suggests a direct cantly suppress the [Ca 2 + ] c transients showing that Ca 2 + influx through the plasma membrane is likely to be involved. relationship between [Ca 2 + ] c and cellular alkalisation (cyto-Verapamil and nifedipine had no inhibitory effects, which plasmic pH and/or vacuolar pH). However, the mechanism behind this possible causal relation remains to be elucidated. suggests that Ca 2 + release from internal stores might not treatment at high pH o acts as a stimulus which induces transient elevations of the cytoplasmic free calcium concentration ([Ca 2 + ] c ).[Ca 2 + ] c has been shown to play an important role in signal perception and transduction in plants. Many environmental and endogenous stimuli are linked to changes in

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“…Particular mention should be made here on transport proteins (Logan et al 1997 ;Delrot et al 2001 ). These are of two types: carrier proteins and channel proteins .…”

Section: Plasma Membranementioning

confidence: 99%

Organization at the Cellular Level

Krishnamurthy

Bahadur

2015

Plant Biology and Biotechnology

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This chapter deals with plant organization at the cellular level which is the fundamental level of biological integration also. Since cells are often considered as the basic structural and functional unit of any biological organism, organization at the cellular level forms the basis for understanding organization at the higher levels. This chapter deals with the structural and functional aspects of cytoplasm and its various endomembrane system, organelles, storage and other cellular chemicals, nucleus and cell wall.

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Plasma membrane transport systems in higher plants: From black boxes to molecular physiology

Cited by 18 publications

References 59 publications

Aquaporin Nt‐TIPa can account for the high permeability of tobacco cell vacuolar membrane to small neutral solutes

Aquaporin Nt‐TIPa can account for the high permeability of tobacco cell vacuolar membrane to small neutral solutes

Ammonium uptake and cellular alkalisation in roots of Arabidopsis thaliana: The involvement of cytoplasmic calcium

Organization at the Cellular Level

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