Plasma methionine depletion and pharmacokinetic properties in mice of methionine γ-lyase from Citrobacter freundii , Clostridium tetani and Clostridium sporogenes

Biochemical, molecular and anti-tumor characterization of L-methionine gamma lyase produced by local Pseudomonas sp. in Egypt

“…The purified MGLs from this bacterial isolate exhibited the same maximum activity at 37 °C, emphasizing the potential activity of this enzyme for in vivo therapeutic applications. Consistently, the highest activities of MGL at 37 °C were reported for the enzyme from A. flavipes and from other various bacterial isolates ( Kudou et al, 2007 , El-Sayed et al, 2016 , Manukhov et al, 2005 , Morozova et al, 2017 , Dias, 1998 ) . Thus, the purified MGL from this bacterial isolate could have the same reactivity and conformational catalytic structure for binding with the substrate and further catalytic process.…”

“…Methionine γ-lyase has been reported to be produced by various fungal and bacterial isolates. The enzyme was purified from a number of bacterial isolates including Aeromonas sp., Pseudomonas putida ; Brevibacterium linens ; Citrobacter freundii ; Lactococcus lactis as well as Clostridium sporogenes ( Tanaka et al, 1985 , Tanaka et al, 1977 , Amarita et al, 2004 , Manukhov et al, 2005 , Martínez-Cuesta et al, 2006 , Morozova et al, 2017 ) . As well as, MGL has been extensively characterized by Aspergillus flavipes ( Khalaf et al, 2009 ).…”

Quorum Sensing Regulates the Production of Methanethiol in Vibrio harveyi

“…To explore the potential enzymes involved in the MeSH and DMS production in V. harveyi BB120, ratified proteins related to L -Met metabolism and the DMSP cycling were used as training protein sequences for BLASTp according to the references [ 21 , 45 , 46 , 47 , 48 , 49 , 50 ]. The genome of V. harveyi BB120 was searched against the above ratified proteins, and the potential functional proteins related to MeSH and DMS metabolism were recognized with specific standards as described previously [ 51 , 52 , 53 ].…”

Targeting Methionine Addiction of Cancer Cells with Methioninase

“…dose of METase decreased L-Met plasma level to less than 8% of the control value [35]. Since then, the METase gene from Pseudomonas putida was cloned and expressed in Escherichia coli cells [13], and the recombinant enzyme (rMETase) has been shown to be effective against all major types of cancer in vitro and in vivo [36][37][38][39][40][41][42][43][44][45][46][47][48]. PEGylation of rMETase allowed an increase in its halflife in the blood circulation and minimized the host immune response in vivo [49].…”