Plasma MicroRNA-126-5p is Associated with the Complexity and Severity of Coronary Artery Disease in Patients with Stable Angina Pectoris

Abstract

Background: Coronary artery disease (CAD) is a major problem worldwide. As an endothelium-enriched microRNA (miRNA), miR-126 has been reported to serve as a potential biomarker of acute myocardial infarction. However, the relationship between miR-126 and the severity of CAD remains unknown. This study was designed to test whether circulating miR-126 levels are associated with the severity of CAD. Methods: The present study enrolled 40 patients who had risk factors for CAD without angiographically significant CAD, and 110 patients presenting with stable angina pectoris, who were validated left main coronary artery disease (LMCA) and/or multi-vessel disease by coronary angiography. The expression levels of plasma miR-126-5p from all enrolled subjects were estimated by quantitative real-time polymerase chain reaction (qRT-PCR). Then, the relationships between plasma miR-126-5p levels, number of diseased vessels and the corresponding Synergy between PCI with Taxus and Cardiac surgery (SYNTAX) score were analyzed. Results: The expression of circulating miR-126-5p was affected by some CAD risk factors including aging, dyslipidemia and DM. Furthermore, plasma miR-126-5p levels were significantly down-regulated in CAD patients with multi-vessel disease, higher SYNTAX score, rather than isolated LMCA and low SYNTAX score. Conclusion: Circulating miR-126-5p has emerged as a potential biomarker for complexity and severity of CAD in patients with stable angina pectoris.

Circulating MicroRNA Profiling in Non-ST Elevated Coronary Artery Syndrome Highlights Genomic Associations with Serial Platelet Reactivity Measurements

“…Importantly, miR-126 has been shown to correlate with PR in patients with ACS and is likely a strong marker of platelet function 15 . MiR-126-5p promotes endothelial proliferation and www.nature.com/scientificreports www.nature.com/scientificreports/ endothelial cell stability and has been implicated in protective properties including limiting atherosclerosis by suppressing Notch1 inhibitor delta-like 1 homolog and reducing thrombogenicity in diabetes mellitus by targeting tissue factor [28][29][30] . It is therefore credible that lower PR (i.e.…”

Protective Effects of MicroRNA-126 on Human Cardiac Microvascular Endothelial Cells Against Hypoxia/Reoxygenation-Induced Injury and Inflammatory Response by Activating PI3K/Akt/eNOS Signaling Pathway

“…MiR-126 has played significant roles in different physiological and pathological processes, including inflammation, blood vessel growth, and cancer through various pathways [24]. Circulating miR-126-5p has been reported as an underlying biomarker for the complexity and severity of Coronary Artery Disease (CAD) in patients with stable angina pectoris [26]. Additionally, the effect of miR-126 on vascular inflammation is regulated by adhesion molecule-1 [27].…”

MicroRNAs as Therapeutic Targets and Clinical Biomarkers in Atherosclerosis

“…Studies have also reported that some miRNAs have differential expression patterns depending on the disease pathology. miR-126 levels are elevated in patients with atrial fibrillation and heart failure [162], while conversely low levels are reported in patients with angina or AMI [151,163,164], highlighting a diverse role for miR-126 in the cardiovascular system. Circulating levels of several 14q32 miRNAs (miR-134, miR-328, miR-370, miR-480 and miR-487a/b) also possess diagnostic value for CAD, AMI and cardiac death, respectively [161,[165][166][167].…”