Plasma MicroRNA 499 as a Biomarker of Acute Myocardial Infarction

Adachi, Taichi; Nakanishi, Masayoshi; Otsuka, Yoritaka; Nishimura, Kunihiro; Hirokawa, Gou; Goto, Yoichi; Nonogi, Hiroshi; Iwai, Naoharu

doi:10.1373/clinchem.2010.144121

Cited by 344 publications

(306 citation statements)

References 16 publications

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“…Following blasticidin selection, cultures were maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum for the duration of the cultures. On days 0, 3,7,10,14,20,28,35,45,60,90, and 120, approximately 3 million cells were removed for RNA collection. The differentiation protocol and sample collection were performed in 3 independent replicates as indicated by Run 1, 2, and 3 in Figs.…”

Section: Cardiomyocyte Differentiationmentioning

confidence: 99%

“…S3). Finally, the expression pattern of miRNAs previously shown to be important in heart development and disease was examined: developmentally regulated miR-1, miR-133a, miR-133b, miR-208a, miR-208b, miR-143, and miR-145 [8]; hypertrophy-associated miR-199a and miR-214 [44]; and a circulating serum biomarker of human myocardial infarction, miR-499 [45,46]. These miRNAs all followed the predicted patterns given their roles in cardiomyocyte function, disease, and development (Fig.…”

Section: Mirna Profiling During Hipsc Differentiationmentioning

confidence: 99%

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Determination of the Human Cardiomyocyte mRNA and miRNA Differentiation Network by Fine-Scale Profiling

Babiarz

Ravon

Sridhar

et al. 2012

Stem Cells and Development

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To gain insight into the molecular regulation of human heart development, a detailed comparison of the mRNA and miRNA transcriptomes across differentiating human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and biopsies from fetal, adult, and hypertensive human hearts was performed. Gene ontology analysis of the mRNA expression levels of the hiPSCs differentiating into cardiomyocytes revealed 3 distinct groups of genes: pluripotent specific, transitional cardiac specification, and mature cardiomyocyte specific. Hierarchical clustering of the mRNA data revealed that the transcriptome of hiPSC cardiomyocytes largely stabilizes 20 days after initiation of differentiation. Nevertheless, analysis of cells continuously cultured for 120 days indicated that the cardiomyocytes continued to mature toward a more adult-like gene expression pattern. Analysis of cardiomyocyte-specific miRNAs (miR-1, miR-133a/b, and miR-208a/b) revealed an miRNA pattern indicative of stem cell to cardiomyocyte specification. A biostatistitical approach integrated the miRNA and mRNA expression profiles revealing a cardiomyocyte differentiation miRNA network and identified putative mRNAs targeted by multiple miRNAs. Together, these data reveal the miRNA network in human heart development and support the notion that overlapping miRNA networks re-enforce transcriptional control during developmental specification.

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Section: Cardiomyocyte Differentiationmentioning

confidence: 99%

Section: Mirna Profiling During Hipsc Differentiationmentioning

confidence: 99%

Determination of the Human Cardiomyocyte mRNA and miRNA Differentiation Network by Fine-Scale Profiling

Babiarz

Ravon

Sridhar

et al. 2012

Stem Cells and Development

View full text Add to dashboard Cite

show abstract

“…Further to this, the role of miR-499 in AMI diagnosis has been supported by Adachi et al in which they measured the plasma concentration of miR-499 in 14 patients with acute coronary syndrome, 15 patients with congestive heart failure (CHF) and 10 patients without any cardiovascular disease. They concluded that miR-499 was exclusive to cardiac tissue with significant increase in plasma level following acute myocardial infarction (AMI) than below the limit of detectable range in plasma with other groups mentioned above [61].…”

Section: S Pokhrel Y Guotian World Journal Of Cardiovascular Diseasesmentioning

confidence: 94%

MicroRNA and Its Role in Cardiovascular Disease

Pokhrel¹,

Guotian²

2017

WJCD

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MicroRNAs play a key role in regulation of gene expression during cardiac development and cardiac remodeling. MicroRNAs that present in bodily fluids may be useful for screening, diagnosis or therapeutic implication as a treatment. MicroRNAs are relatively new approach targets for researchers and clinicians in today world. MicroRNAs are small noncoding RNA (ncRNA) having approximately 21 to 25 nucleotides in length, and they mainly act as a transcriptional regulators of gene expression in diverse biological processes such as cellular proliferation, differentiation, tumorigenesis to death and so on. There is no doubt that lethiferous cardiac disease is one of the most common causes of deaths worldwide. MicroRNAs may regulate in several cardiovascular pathologies, not only limited to hypertrophy, heart failure, arrhythmias, hypertension, myocardial infarction, dyslipidemias and congenital heart diseases, but in circulation and bodily fluids are potential novel biomarkers for above mentioned cardiac pathologies. Knowing abnormalities in genetic level, early and accurate detection, effective treatment and prevention is the ideal management of cardiovascular diseases in today's world. However, every detail of an individual microRNA and their system is huge and beyond the scope of this article. Therefore, in this review we try to cover the overall major aspects of the microRNAs and its role in cardiovascular system.

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“…There was an increase of more than 1,000-fold in acute myocardial infarction 6,[37][38][39] of miRs-208b and-499. In this condition, they reflect tissue injury and cell death.…”

Section: Microrna As Biomarker In Heart Failurementioning

confidence: 96%

Exercício físico e microRNAs: novas fronteiras na insuficiência cardíaca

Fernandes-Silva¹,

Carvalho²,

Guimarães³

et al. 2012

Arq. Bras. Cardiol.

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Although the impact of exercise on survival of patients with heart failure has been recently questioned, exercise training improves quality of life, functional capacity, inflammation, endothelial and autonomic function. In recent years, interest has increased regarding a group of small non-protein coding RNAs called microRNAs.Studies have shown that the expression of these molecules changes in several pathological conditions, such as myocardial infarction, myocardial ischemia and heart failure, and when clinical improvement occurs, they seem to normalize. With the potential for practical applicability, markers that may be useful in diagnostic and prognostic assessment of heart failure have been identified, such as miR-423-5p. In addition, results of experimental studies have indicated that there are potential therapeutic effects of microRNAs.MicroRNAs are involved in the regulation of gene expression during fetal development and in adult individuals, increasing or decreasing in the heart in response to physiological stress, injury or hemodynamic overload. Thus, the study of the behavior of these molecules in physical exercise has brought important information about the effects of this therapeutic modality and represents a new era in the understanding of heart failure.This review aims to integrate the evidence on microRNAs in heart failure with greater relevance in the study of physical exercise.

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Plasma MicroRNA 499 as a Biomarker of Acute Myocardial Infarction

Cited by 344 publications

References 16 publications

Determination of the Human Cardiomyocyte mRNA and miRNA Differentiation Network by Fine-Scale Profiling

Determination of the Human Cardiomyocyte mRNA and miRNA Differentiation Network by Fine-Scale Profiling

MicroRNA and Its Role in Cardiovascular Disease

Exercício físico e microRNAs: novas fronteiras na insuficiência cardíaca

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