Plasma N-acetyl-β-glucosaminidase and β-glucuronidase in health and disease

Woollen, J. W.; Turner, P

doi:10.1016/0009-8981(65)90149-x

Cited by 102 publications

(27 citation statements)

References 27 publications

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“…As stated in the introduction, Woolen and Turner (6) showed that there was a significant correlation between plasma (3-glucuronidase activity and blood sugar level in the diabetic patient. However, other investigators could not demonstrate such a phenomenon and also the significancy shown by them was lower than that obtained by us in alloxan diabetic and normal rabbits.…”

Section: Discussionmentioning

confidence: 90%

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SERUM β-GLUCURONIDASE and β-ACETYLGLUCOSAMINASE ACTIVITIES IN EXPERIMENTAL DIABETIC RABBITS

Shioya

Ohishi

Kakimoto

1968

Japanese Journal of Pharmacology

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In a previous paper (1), the authors demonstrated that changes in serum Q-glucuroni dase activity paralleled blood sugar levels after the administration of glucose, epinephrine and insulin, while an inverse relationship was found with hydrocortisone treatment. Furthermore, we observed that the changes in serum Q-acetylglucosaminase activity in such cases were somewhat different from that of j -glucuronidase.On the other hand, several investigators (2-7) have shown an increase in serum Q glucuronidase activity of diabetic patients. Among them, however, only Woolen and Turner (6) has observed a significant correlation between plasma Q-glucuronidase activity and blood sugar levels.In the present study we have extended our survey of the relationship between serum Q-glucuronidase activity and the blood sugar level in experimental diabetic rabbits, pre pared by a single injection of alloxan or consecutive administrarion of hydrocortisone. MATERIALS AND METHODS Experimental animalsWhite male rabbits bred in our laboratories, weighing from 2.8 to 4.0 kg, were used. The animals were kept in a constant temperature (24:t2°C) and humidity controlled (50 to 70%) room.Alloxan diabetes was induced by a single intravenous injection of 100 mg/kg of re crystallized alloxan monohydrate as a 5 % solution, and cortisone diabetes was produced by the consecutive intramuscular injections of hydrocortisone acetate at a dose of 20 mg/ animal/day. Each animal was allowed to drink water ad libitum and offered 100-150 g of RC-5 (Oriental Yeast Co., Ltd., Tokyo) and 50-100 g of carrots a day, the quantities being determined by what was eaten in a few hours and sufficinet to maintain the diabetic state. The animals were placed in individual, screen-bottomed metabolism cages set over large funnels permitting quantitative collection of urine under toluen.Changes in serum (3-glucuronidase and Q-acetylglucosaminase activities in diabetic rabbits Blood sugar level, serum (3-glucuronidase and (3-acetylglucosaminase activities were determined once a week. Blood samples were drawn from the ear vein of animals in

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Section: Discussionmentioning

confidence: 90%

“…Among them, however, only Woolen and Turner (6) has observed a significant correlation between plasma Q-glucuronidase activity and blood sugar levels.…”

mentioning

confidence: 99%

SERUM β-GLUCURONIDASE and β-ACETYLGLUCOSAMINASE ACTIVITIES IN EXPERIMENTAL DIABETIC RABBITS

Shioya

Ohishi

Kakimoto

1968

Japanese Journal of Pharmacology

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“…Previous work has shown that glycosidase activity is higher in young tissue (22,23), and that in patients with certain tumors (including lung, stomach, prostate, and breast) the serum activity of certain glycosidases is increased (24)(25)(26). The present work is consistent with these earlier reports and extends them to human malignant tissues.…”

Section: Resultsmentioning

confidence: 99%

Enzyme Activity in Invasive Tumors of Human Breast and Colon

Bosmann

Hall

1974

Proc. Natl. Acad. Sci. U.S.A.

173

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“…The distribution of these enzymes has been documented for mammalian liver (Weissmann et al, 1967), cerebellum (Bosmann and Merritt, 1969), and testes (Caygill, Roston and Jevons, 1966). An elevation of certain glycosidases in fibroblasts transformed by oncogenic viruses (Bosmann, , 1972 and in the sera of patients with carcinoma of the lung, stomach, prostate and breast (Goldbarg et al, 1959;Woollen and Turner, 1965;Ayoub, 1967) have been reported. The present work has been carried out to study the normal distribution of glycosidases and fi-D-glucuronidase in various rat and mouse tissues, and to investigate the effect of 1,2-dimethylhydrazine dihydrochloride (DMH) on these enzymes since this carcinogen has been found to produce rat colonic tumours and mouse colonic adenomatous polyps which have a characteristic loss of mucopolysaccharides (Rogers, Herndon and Newberne, 1973;Haase et al, 1973).…”

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confidence: 99%

Glycosidases in Normal and Dimethylhydrazine-Treated Rats and Mice with Special Reference to the Colonic Tumours

Mian¹,

Cowen²

1974

Br J Cancer

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Summary.-Activities of 12 glycosidases and of P-D-glucuronidase were measured in liver, kidney and in the gastrointestinal tract of rats and mice. The activities of different enzymes varied not only within one tissue but also among different tissues. In rats injected with 1,2 -dimethylhydrazine, a many fold increase indase and a-L-fucosidase was fountl in colonic tumours and colonic mucosa. These enzymes were elevated significantly in the kidney of tumour bearing animals as well. Liver and other parts of the gastrointestinal tract showed an increase only in N-acetyl-hexosaminidase with the appearance of colonic tumours. In treated mice, 2 N-acetylhexosaminidases were elevated in colon, duodenum, liver and kidney. However, in liver and kidney, P-D-galactosidase was also significantly increased.

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Plasma N-acetyl-β-glucosaminidase and β-glucuronidase in health and disease

Cited by 102 publications

References 27 publications

SERUM β-GLUCURONIDASE and β-ACETYLGLUCOSAMINASE ACTIVITIES IN EXPERIMENTAL DIABETIC RABBITS

SERUM β-GLUCURONIDASE and β-ACETYLGLUCOSAMINASE ACTIVITIES IN EXPERIMENTAL DIABETIC RABBITS

Enzyme Activity in Invasive Tumors of Human Breast and Colon

Glycosidases in Normal and Dimethylhydrazine-Treated Rats and Mice with Special Reference to the Colonic Tumours

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