Plasma Nucleotide Dynamics during Exercise and Recovery in Highly Trained Athletes and Recreationally Active Individuals

Abstract

Circulating plasma ATP is able to regulate local skeletal muscle blood flow and 02 delivery causing considerable vasodilatation during exercise. We hypothesized that sport specialization and specific long-term training stimuli have an impact on venous plasma [ATP] and other nucleotides concentration. Four athletic groups consisting of sprinters (n=11; age range 21–30 yr), endurance-trained athletes (n=16; age range 18–31 yr), futsal players (n=14; age range 18–30 yr), and recreationally active individuals (n=12; age range 22–33 yr) were studied. Venous blood samples were collected at rest, during an incremental treadmill test, and during recovery. Baseline [ATP] was 759±80 nmol·l−1 in competitive athletes and 680±73 nmol·l−1 in controls and increased during exercise by ~61% in competitive athletes and by ~31% in recreationally active participants. We demonstrated a rapid increase in plasma [ATP] at exercise intensities of 83–87% of VO2max in competitive athletes and 94% in controls. Concentrations reported after 30 minutes of recovery were distinct from those obtained preexercise in competitive athletes (P < 0.001) but not in controls (P = 0.61). We found a correlation between total-body skeletal muscle mass and resting and maximal plasma [ATP] in competitive athletes (r=0.81 and r=0.75, respectively). In conclusion, sport specialization is significantly related to plasma [ATP] at rest, during exercise, and during maximal effort. Intensified exercise-induced plasma [ATP] increases may contribute to more effective vessel dilatation during exercise in highly trained athletes than in recreational runners. The most rapid increase in ATP concentration was associated with the respiratory compensation point. No differences between groups of competitive athletes were observed during the recovery period suggesting a similar pattern of response after exercise. Total-body skeletal muscle mass is indirectly related to plasma [ATP] in highly trained athletes.

Physical and Physiological Match-Play Demands and Player Characteristics in Futsal: A Systematic Review

“…In addition, as reported above, players achieve mean and peak VO 2 values during competition which correspond to their 76 and 99% of VO 2max , respectively. Upon review, 31 studies (Barbero-Alvarez et al, 2009 ; Gorostiaga et al, 2009 ; Baroni and Leal Junior, 2010 ; Castagna and Barbero-Alvarez, 2010 ; Milanez et al, 2011 ; Makaje et al, 2012 ; Boullosa et al, 2013 ; Oliveira et al, 2013 ; Pedro et al, 2013 ; Cuadrado-Peñafiel et al, 2014 ; Miloski et al, 2014 ; Soares-Caldeira et al, 2014 ; De Freitas et al, 2015 , 2019 ; Galy et al, 2015 ; Garcia-Tabar et al, 2015 ; Charlot et al, 2016 ; Floriano et al, 2016 ; Nakamura et al, 2016 , 2018 ; Naser and Ali, 2016 ; Barbieri et al, 2017 ; Barcelos et al, 2017 ; Valladares-Rodriguez et al, 2017 ; Nogueira et al, 2018 ; Zarebska et al, 2018 , 2019 ; Farhani et al, 2019 ; Nikolaidis et al, 2019 ; Teixeira et al, 2019 ; Wlodarczyk et al, 2019 , 2020 ; Bekris et al, 2020 ) have looked at the physiological characteristics of elite futsal players ( Table 2 ).…”

Alterations in Exercise-Induced Plasma Adenosine Triphosphate Concentration in Highly Trained Athletes in a One-Year Training Cycle

“…ADP also activates a negative feedback pathway of ATP release from erythrocytes via P2Y 13 receptors [11]. Therefore, an increased ADP concentration during exercise [12] may be of great physiological importance to diminishing ATP release. Plasma adenosine monophosphate (AMP), alongside from ATP and ADP, has also been shown to increase during submaximal and maximal exercise intensity [13].…”

“…Futsal training requires, in turn, multiple moments of high blood flow delivery during prolonged exercise. A recent study showed the impact of sport specialization on exercise-induced plasma ([ATP]) in highly trained athletes, and indicated that total-body skeletal muscle mass is an important factor [12]. We believe there is a need for further research addressing the effect of long-term whole-body training on vascular function, and in particular, its influence on ATP and its derivatives.…”

Acute moderate‐intensity aerobic exercise promotes purinergic and inflammatory responses in sedentary, overweight and physically active subjects

“…Correlation analyses were performed using the Pearson's correlation coefficient (P < 0.05) similar to physically active individuals. Previously, it was demonstrated that treadmill exercise at intensities from 83% to 94% of maximum oxygen uptake (V O 2 max ) increased the plasma levels of ATP in highly trained and physically active male subjects (Zarębska et al, 2018). The modulatory effects of acute and long-term exercise models on purinergic enzymes have been reported in previous studies.…”